Chicken dapper genes are versatile markers for mesodermal tissues, embryonic muscle stem cells, neural crest cells, and neurogenic placodes

2009 ◽  
Vol 238 (5) ◽  
pp. 1166-1178 ◽  
Author(s):  
Lúcia Elvira Alvares ◽  
Farrah Leigh Winterbottom ◽  
Débora Rodrigues Sobreira ◽  
José Xavier-Neto ◽  
Frank Richard Schubert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Muscle Stem Cells ◽  
Embryonic Muscle

scholarly journals Use of adenovirus for ectopic gene expression inXenopus: Chicken dapper genes are versatile markers for mesodermal tissues, embryonic muscle stem cells, neural crest cells, and neurogenic placodes

2009 ◽  
Vol 238 (7) ◽  
pp. spcone-spcone
Author(s):  
Lúcia Elvira Alvares ◽  
Farrah Leigh Winterbottom ◽  
Débora Rodrigues Sobreira ◽  
José Xavier-Neto ◽  
Frank Richard Schubert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Muscle Stem Cells ◽  
Ectopic Gene Expression ◽  
Embryonic Muscle

Chicken dapper genes are versatile markers for mesodermal tissues, embryonic muscle stem cells, neural crest cells, and neurogenic placodes

2009 ◽  
Vol 238 (11) ◽  
pp. 2948-2948
Author(s):  
Lúcia Elvira Alvares ◽  
Farrah Leigh Winterbottom ◽  
Erika Cristina Jorge ◽  
Débora Rodrigues Sobreira ◽  
José Xavier-Neto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Muscle Stem Cells ◽  
Embryonic Muscle

scholarly journals SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells

2021 ◽  
Author(s):  
Rui-fang Li ◽  
Guo-xin Nan ◽  
Dan Wang ◽  
Chang Gao ◽  
Juan Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Stem Cells ◽  
Neural Crest ◽  
Schwann Cells ◽  
Early Development ◽  
Glial Cells ◽  
Embryonic Stem ◽  
Specific Effect ◽  
Neural Crest Cells

Background: The specific effect of SV40T on neurocytes has been rarely investigated by the researchers. We transfected Schwann cells (SCs) that did not have differentiation ability with MPH 86 plasmid containing SV40T in order to explore the effects of SV40T on Schwann cells.Methods: SCs were transfected with MPH 86 plasmid carrying the SV40T gene and cultured in different media, as well as co-cultured with neural stem cells (NSCs). In our study, SCs overexpressing SV40T were defined as SV40T-SCs. The proliferation of these cells was detected by WST-1, and the expression of different biomarkers was analyzed by qPCR and immunohistochemistry. Results: SV40T induced the characteristics of NSCs, such as the ability to grow in suspension, form spheroid colonies and proliferate rapidly, in the SCs, which were reversed by knocking out SV40T by the Flip-adenovirus. In addition, SV40T upregulated the expressions of neural crest-associated markers Nestin, Pax3 and Slug, and down-regulated S100b as well as the markers of mature SCs MBP, GFAP and Olig1/2. These cells also expressed NSC markers like Nestin, Sox2, CD133 and SSEA-1, as well as early development markers of embryonic stem cells (ESCs) like BMP4, c-Myc, OCT4 and Gbx2. Co-culturing with NSCs induced differentiation of the SV40T-SCs into neuronal and glial cells. Conclusions: SV40T reprograms Schwann cells to stem-like cells at the stage of neural crest cells (NCCs) that can differentiate to neurocytes.

scholarly journals Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells

2019 ◽  
Vol 28 (1) ◽  
pp. 28-43 ◽  
Author(s):  
Shota Fujii ◽  
Satoru Yoshida ◽  
Emi Inagaki ◽  
Shin Hatou ◽  
Kazuo Tsubota ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Crest Cells ◽  
Immunological Properties ◽  
Induced Pluripotent

scholarly journals Applications of Mesenchymal Stem Cells and Neural Crest Cells in Craniofacial Skeletal Research

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Satoru Morikawa ◽  
Takehito Ouchi ◽  
Shinsuke Shibata ◽  
Takumi Fujimura ◽  
Hiromasa Kawana ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Tube Formation ◽  
Culture Conditions ◽  
Adherent Culture ◽  
Fundamental Biology ◽  
Craniofacial Tissue ◽  
Human Specific

Craniofacial skeletal tissues are composed of tooth and bone, together with nerves and blood vessels. This composite material is mainly derived from neural crest cells (NCCs). The neural crest is transient embryonic tissue present during neural tube formation whose cells have high potential for migration and differentiation. Thus, NCCs are promising candidates for craniofacial tissue regeneration; however, the clinical application of NCCs is hindered by their limited accessibility. In contrast, mesenchymal stem cells (MSCs) are easily accessible in adults, have similar potential for self-renewal, and can differentiate into skeletal tissues, including bones and cartilage. Therefore, MSCs may represent good sources of stem cells for clinical use. MSCs are classically identified under adherent culture conditions, leading to contamination with other cell lineages. Previous studies have identified mouse- and human-specific MSC subsets using cell surface markers. Additionally, some studies have shown that a subset of MSCs is closely related to neural crest derivatives and endothelial cells. These MSCs may be promising candidates for regeneration of craniofacial tissues from the perspective of developmental fate. Here, we review the fundamental biology of MSCs in craniofacial research.

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