scholarly journals Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron

2007 ◽  
Vol 236 (11) ◽  
pp. 3071-3076 ◽  
Author(s):  
Melissa E. Hardy ◽  
Louis V. Ross ◽  
Chi-Bin Chien
Keyword(s):  
Heat Shock ◽  
Local Heat ◽  
Zebrafish Embryos ◽  
Soldering Iron

Nonlinear deformation of cylindrical shell with local heat shock

1990 ◽  
Vol 26 (11) ◽  
pp. 1068-1072
Author(s):  
N. I. Obodan ◽  
N. B. Makarenko
Keyword(s):  
Cylindrical Shell ◽  
Heat Shock ◽  
Nonlinear Deformation ◽  
Local Heat

DNA transformation via local heat shock

2007 ◽  
Vol 91 (1) ◽  
pp. 013902 ◽  
Author(s):  
Sha Li ◽  
L. Meadow Anderson ◽  
Jui-Ming Yang ◽  
Liwei Lin ◽  
Haw Yang
Keyword(s):  
Heat Shock ◽  
Local Heat ◽  
Dna Transformation

DNA transformation by local heat shock

2007 ◽  
Author(s):  
Sha Li ◽  
L. Meadow Anderson ◽  
Liwei Lin ◽  
Haw Yang
Keyword(s):  
Heat Shock ◽  
Local Heat ◽  
Dna Transformation

Improvement of nutritive perfusion after free tissue transfer by local heat shock-priming-induced preservation of capillary flowmotion

2005 ◽  
Vol 123 (1) ◽  
pp. 102-108 ◽  
Author(s):  
Martin Rücker ◽  
Bülent Kadirogullari ◽  
Brigitte Vollmar ◽  
Wolfgang J. Spitzer ◽  
Michael D. Menger
Keyword(s):  
Heat Shock ◽  
Free Tissue Transfer ◽  
Local Heat ◽  
Tissue Transfer ◽  
Nutritive Perfusion

scholarly journals Effects of repeated local heat therapy on skeletal muscle structure and function in humans

2020 ◽  
Vol 128 (3) ◽  
pp. 483-492 ◽  
Author(s):  
Kyoungrae Kim ◽  
Blake A. Reid ◽  
Caitlin A. Casey ◽  
Brooke E. Bender ◽  
Bohyun Ro ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Heat Shock ◽  
Endothelial Nitric Oxide Synthase ◽  
Local Heat ◽  
Mitochondrial Content ◽  
Heat Therapy ◽  
Shock Proteins ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

The purpose of the present study was to examine the effects of repeated exposure to local heat therapy (HT) on skeletal muscle function, myofiber morphology, capillarization, and mitochondrial content in humans. Twelve young adults (23.6 ± 4.8 yr, body mass index 24.9 ± 3.0 kg/m2) had one randomly selected thigh treated with HT (garment perfused with water at ~52°C) for 8 consecutive weeks (90 min, 5 days/wk) while the opposite thigh served as a control. Biopsies were obtained from the vastus lateralis muscle before and after 4 and 8 wk of treatment. Knee extensor strength and fatigue resistance were also assessed using isokinetic dynamometry. The changes in peak isokinetic torque were higher ( P = 0.007) in the thigh exposed to HT than in the control thigh at weeks 4 (control 4.2 ± 13.1 Nm vs. HT 9.1 ± 16.1 Nm) and 8 (control 1.8 ± 9.7 Nm vs. HT 7.8 ± 10.2 Nm). Exposure to HT averted a temporal decline in capillarization around type II fibers ( P < 0.05), but had no effect on capillarization indexes in type I fibers. The content of endothelial nitric oxide synthase was ~18% and 35% higher in the thigh exposed to HT at 4 and 8 wk, respectively ( P = 0.003). Similarly, HT increased the content of small heat shock proteins HSPB5 ( P = 0.007) and HSPB1 ( P = 0.009). There were no differences between thighs for the changes in fiber cross-sectional area and mitochondrial content. These results indicate that exposure to local HT for 8 wk promotes a proangiogenic environment and enhances muscle strength but does not affect mitochondrial content in humans. NEW & NOTEWORTHY We demonstrate that repeated application of heat therapy to the thigh with a garment perfused with warm water enhances the strength of knee extensors and influences muscle capillarization in parallel with increases in the content of endothelial nitric oxide synthase and small heat shock proteins. This practical method of passive heat stress may be a feasible tool to treat conditions associated with capillary rarefaction and muscle weakness.

scholarly journals Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element

2001 ◽  
Vol 356 (2) ◽  
pp. 353-359 ◽  
Author(s):  
Alfredo MOLINA ◽  
Emmanuel Di MARTINO ◽  
Joseph A. MARTIAL ◽  
Marc MULLER
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Transient Expression ◽  
Heat Shock Response ◽  
Heat Shock Protein 70 ◽  
Zebrafish Embryos ◽  
Epithelioma Papulosum Cyprini ◽  
Hsp70 Promoter ◽  
Shock Response ◽  
Constitutive Factor

We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. −800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.

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