DNA transformation via local heat shock

2007 ◽  
Vol 91 (1) ◽  
pp. 013902 ◽  
Author(s):  
Sha Li ◽  
L. Meadow Anderson ◽  
Jui-Ming Yang ◽  
Liwei Lin ◽  
Haw Yang
2007 ◽  
Author(s):  
Sha Li ◽  
L. Meadow Anderson ◽  
Liwei Lin ◽  
Haw Yang

1990 ◽  
Vol 26 (11) ◽  
pp. 1068-1072
Author(s):  
N. I. Obodan ◽  
N. B. Makarenko

2005 ◽  
Vol 123 (1) ◽  
pp. 102-108 ◽  
Author(s):  
Martin Rücker ◽  
Bülent Kadirogullari ◽  
Brigitte Vollmar ◽  
Wolfgang J. Spitzer ◽  
Michael D. Menger

2020 ◽  
Vol 128 (3) ◽  
pp. 483-492 ◽  
Author(s):  
Kyoungrae Kim ◽  
Blake A. Reid ◽  
Caitlin A. Casey ◽  
Brooke E. Bender ◽  
Bohyun Ro ◽  
...  

The purpose of the present study was to examine the effects of repeated exposure to local heat therapy (HT) on skeletal muscle function, myofiber morphology, capillarization, and mitochondrial content in humans. Twelve young adults (23.6 ± 4.8 yr, body mass index 24.9 ± 3.0 kg/m2) had one randomly selected thigh treated with HT (garment perfused with water at ~52°C) for 8 consecutive weeks (90 min, 5 days/wk) while the opposite thigh served as a control. Biopsies were obtained from the vastus lateralis muscle before and after 4 and 8 wk of treatment. Knee extensor strength and fatigue resistance were also assessed using isokinetic dynamometry. The changes in peak isokinetic torque were higher ( P = 0.007) in the thigh exposed to HT than in the control thigh at weeks 4 (control 4.2 ± 13.1 Nm vs. HT 9.1 ± 16.1 Nm) and 8 (control 1.8 ± 9.7 Nm vs. HT 7.8 ± 10.2 Nm). Exposure to HT averted a temporal decline in capillarization around type II fibers ( P < 0.05), but had no effect on capillarization indexes in type I fibers. The content of endothelial nitric oxide synthase was ~18% and 35% higher in the thigh exposed to HT at 4 and 8 wk, respectively ( P = 0.003). Similarly, HT increased the content of small heat shock proteins HSPB5 ( P = 0.007) and HSPB1 ( P = 0.009). There were no differences between thighs for the changes in fiber cross-sectional area and mitochondrial content. These results indicate that exposure to local HT for 8 wk promotes a proangiogenic environment and enhances muscle strength but does not affect mitochondrial content in humans. NEW & NOTEWORTHY We demonstrate that repeated application of heat therapy to the thigh with a garment perfused with warm water enhances the strength of knee extensors and influences muscle capillarization in parallel with increases in the content of endothelial nitric oxide synthase and small heat shock proteins. This practical method of passive heat stress may be a feasible tool to treat conditions associated with capillary rarefaction and muscle weakness.


2001 ◽  
Vol 88 (3) ◽  
pp. 450-457 ◽  
Author(s):  
M. Rücker ◽  
T. Schäfer ◽  
F. Roesken ◽  
W. J. Spitzer ◽  
M. Bauer ◽  
...  

2006 ◽  
Vol 26 (7) ◽  
pp. 1632-1639 ◽  
Author(s):  
Martin Rücker ◽  
Thilo Schäfer ◽  
Claudia Scheuer ◽  
Yves Harder ◽  
Brigitte Vollmar ◽  
...  

2018 ◽  
Vol 771 ◽  
pp. 124-129 ◽  
Author(s):  
Arkady A. Skvortsov ◽  
Danila E. Pshonkin ◽  
Mikhail N. Luk'yanov

The work is devoted to the study of defect formation processes in the near-surface layers of silicon under thermal shock conditions and to the effect of preliminary exposure in a constant magnetic field on this process. As a result of investigations it was established that dislocation half-loops near the local heat source are formed in the near-surface layers of Si (with a depth of up to 30 μm) after a current pulse of density j> 5.1010 A / m2 passing through the metallized film on the silicon surface. In addition, it was found that preliminary exposure of samples in a constant magnetic field leads to an increase in the dislocation density compared to samples not exposed in a magnetic field.


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