scholarly journals Transcriptional and spatiotemporal regulation of prolactin receptor mRNA and cooperativity with progesterone receptor function during ductal branch growth in the mammary gland

2001 ◽  
Vol 222 (2) ◽  
pp. 192-205 ◽  
Author(s):  
Russell C. Hovey ◽  
Josephine F. Trott ◽  
Erika Ginsburg ◽  
Anita Goldhar ◽  
Mark M. Sasaki ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Progesterone Receptor ◽  
Prolactin Receptor ◽  
Receptor Function ◽  
Receptor Mrna ◽  
Branch Growth ◽  
Spatiotemporal Regulation

Changes in the gene expression of progesterone receptor and prolactin receptor in sow mammary gland between late gestation and lactation

1998 ◽  
Vol 1998 ◽  
pp. 179-179
Author(s):  
H. M. Miller ◽  
W. T. Dixon ◽  
G. R. Foxcroft ◽  
F. X. Aherne
Keyword(s):  
Progesterone Receptor ◽  
Prolactin Receptor ◽  
Plasma Concentrations ◽  
Progesterone Receptors ◽  
Late Gestation ◽  
Rapid Decline ◽  
Plasma Prolactin ◽  
Early Lactation ◽  
Plasma Progesterone ◽  
Receptor Mrna

Lactogenesis is triggered by a rapid decline in plasma progesterone concentration combined with a peak in plasma prolactin concentration; in mice, there is a concurrent loss of mammary progesterone receptors (Haslam and Shyamala, 1980). The aims of this experiment were to determine the pattern of change of progesterone receptor and prolactin receptor mRNA during late gestation and early lactation and to determine whether abundance of mRNA for the two receptors are related to each other, to plasma concentrations of progesterone and prolactin or to piglet performance.

Gene expression of leptin, leptin receptor, prolactin receptor and whey acidic protein in mammary glands of late-pregnant gilts from two breeds

2004 ◽  
Vol 84 (4) ◽  
pp. 621-629 ◽  
Author(s):  
M. F. Palin ◽  
D. Beaudry ◽  
C. Farmer
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Leptin Receptor ◽  
Prolactin Receptor ◽  
Mammary Glands ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Mrna Levels ◽  
Parenchymal Tissue ◽  
Receptor Mrna

In order to identify genes which are essential for pig mammary gland development, mRNA levels of prolactin receptor (PRL-R), leptin, leptin receptor and whey acidic protein (WAP) were measured in parenchymal tissue of 110-d-pregnant gilts. Thirteen Upton-Meishan (UM) and 14 Large White (LW) pregnant gilts and 5 non-pregnant control gilts (2 LW and 3UM) were used. PRL-R and WAP mRNA levels were higher in pregnant than in non-pregnant gilts (P < 0.05). Leptin mRNA levels were higher in UM than in LW gilts (P < 0.05), but this breed effect was not seen when leptin mRNA levels were corrected for percent fat in parenchyma. Correlations were found between concentrations of IGF-I in plasma and PRL-R (P < 0.01) and WAP (P < 0.05) mRNA levels in UM gilts. Serum prolactin (PRL) was correlated with leptin mRNA levels in the overall (P < 0.05) and LW (P < 0.05) populations of gilts, while estradiol was associated with leptin receptor mRNA in UM gilts (P < 0.05). The mRNA levels of all studied genes were positively correlated with mammary parenchymal and extra parenchymal weights in UM gilts, whereas these variables were only correlated with PRL-R and WAP gene expression in LW gilts. The presence of leptin and leptin receptor mRNA in parenchymal tissue suggests a paracrine role for leptin in mammary tissue of late-pregnant gilts. These results also suggest that the PRL signalling pathway is fully active at the transcriptional level in the mammary gland of gilts at 110 d of pregnancy. Key words: Genetics, pig, mammary glands, Meishan, mRNA

scholarly journals Effects of Weaning and Suckling on γ-Casein and Prolactin Receptor mRNA Levels in the Mouse Mammary Gland during Lactation

1998 ◽  
Vol 69 (8) ◽  
pp. 728-733
Author(s):  
Jae-Young KIM ◽  
Yasushi MIZOGUCHI ◽  
Takeshi KURAISHI ◽  
Hirohito YAMAGUCHI ◽  
Jumpei ENAMI ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Prolactin Receptor ◽  
Mouse Mammary Gland ◽  
Mrna Levels ◽  
Receptor Mrna

Changes in the gene expression of progesterone receptor and prolactin receptor in sow mammary gland between late gestation and lactation

1998 ◽  
Vol 1998 ◽  
pp. 179-179
Author(s):  
H. M. Miller ◽  
W. T. Dixon ◽  
G. R. Foxcroft ◽  
F. X. Aherne
Keyword(s):  
Progesterone Receptor ◽  
Prolactin Receptor ◽  
Plasma Concentrations ◽  
Progesterone Receptors ◽  
Late Gestation ◽  
Rapid Decline ◽  
Plasma Prolactin ◽  
Early Lactation ◽  
Plasma Progesterone ◽  
Receptor Mrna

Lactogenesis is triggered by a rapid decline in plasma progesterone concentration combined with a peak in plasma prolactin concentration; in mice, there is a concurrent loss of mammary progesterone receptors (Haslam and Shyamala, 1980). The aims of this experiment were to determine the pattern of change of progesterone receptor and prolactin receptor mRNA during late gestation and early lactation and to determine whether abundance of mRNA for the two receptors are related to each other, to plasma concentrations of progesterone and prolactin or to piglet performance.

scholarly journals Progesterone Receptor Isoforms A and B: Temporal and Spatial Differences in Expression during Murine Mammary Gland Development

Endocrinology ◽  
2005 ◽  
Vol 146 (8) ◽  
pp. 3577-3588 ◽  
Author(s):  
Mark D. Aupperlee ◽  
Kyle T. Smith ◽  
Anastasia Kariagina ◽  
Sandra Z. Haslam
Keyword(s):  
Mammary Gland ◽  
Progesterone Receptor ◽  
Cyclin D1 ◽  
Mammary Gland Development ◽  
Minor Role ◽  
Normal Mammary Gland ◽  
Temporal Separation ◽  
Stage Of Development ◽  
Gland Development

Abstract Progesterone is a potent mitogen in the mammary gland. Based on studies using cells and animals engineered to express progesterone receptor (PR) isoforms A or B, PRA and PRB are believed to have different functions. Using an immunohistochemical approach with antibodies specific for PRA only or PRB only, we show that PRA and PRB expression in mammary epithelial cells is temporally and spatially separated during normal mammary gland development in the BALB/c mouse. In the virgin mammary gland when ductal development is active, the only PR protein isoform expressed was PRA. PRA levels were significantly lower during pregnancy, suggesting a minor role at this stage of development. PRB was abundantly expressed only during pregnancy, during alveologenesis. PRA and PRB colocalization occurred in only a small percentage of cells. During pregnancy there was extensive colocalization of PRB with 5-bromo-2′-deoxyuridine (BrdU) and cyclin D1; 95% of BrdU-positive cells and 83% of cyclin D1-positive cells expressed PRB. No colocalization of PRA with either BrdU or cyclin D1 was observed at pregnancy. In the virgin gland, PRA colocalization with BrdU or cyclin D1 was low; only 27% of BrdU-positive cells and 4% of cyclin D1-positive cells expressed PRA. The implication of these findings is that different actions of progesterone are mediated in PRB positive vs. PRA-positive cells in vivo. The spatial and temporal separation of PR isoform expression in mouse mammary gland provides a unique opportunity to determine the specific functions of PRA vs. PRB in vivo.

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