Magnetic beads as an extraction medium for simultaneous quantification of acetaminophen and structurally related compounds in human serum

2014 ◽  
Vol 7 (6) ◽  
pp. 457-466 ◽  
Author(s):  
Caroline Bylda ◽  
Vanya Velichkova ◽  
Jens Bolle ◽  
Roland Thiele ◽  
Uwe Kobold ◽  
...  
Keyword(s):  
Human Serum ◽  
Magnetic Beads ◽  
Simultaneous Quantification ◽  
Related Compounds

Simultaneous quantification of acetaminophen and structurally related compounds in human serum and plasma

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Caroline Bylda ◽  
Roland Thiele ◽  
Uwe Kobold ◽  
Dietrich A. Volmer
Keyword(s):  
Human Serum ◽  
Simultaneous Quantification ◽  
Related Compounds

A Diagnostic Platform for Rapid, Simultaneous Quantification of Procalcitonin and C-Reactive Protein in Human Serum

2021 ◽  
Author(s):  
Xiangkun Cao ◽  
Ongagna Y. Serge Yvon ◽  
Ruisheng Wang ◽  
Yue Ren ◽  
Balaji Srinivasan ◽  
...  
Keyword(s):  
Human Serum ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Simultaneous Quantification

scholarly journals Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Xiang Chen ◽  
Qiyang Zhou ◽  
Ting Zhang ◽  
ChunXin Wang ◽  
Zheng Yu ◽  
...  
Keyword(s):  
Folic Acid ◽  
Horseradish Peroxidase ◽  
Human Serum ◽  
Magnetic Beads ◽  
Megaloblastic Anemia ◽  
Human Growth ◽  
Cross Reactivity ◽  
Alkaline Condition ◽  
Folate Binding Protein ◽  
Chemiluminescence Immunoassay

Folic acid (FA) is an important vitamin for human growth, especially for pregnant women. FA deficiency is associated with megaloblastic anemia, neural tube defects, cardiovascular diseases, irritability, diarrhea, and psychiatric disorders. Normally, FA molecules bind to folate-binding protein (FBP) in the serum as complex. Before quantify the FA concentration, a releasing procedure should be conducted. Alkaline condition and tris(2-carboxyethyl)phosphine (TCEP) are used to release binding FA to freeing state. In this work, a chemiluminescence immunoassay (CLIA) for human serum FA was established by competition model. Streptavidin (SA) was labeled to magnetic beads by an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDAC/NHS) method. Activated biotin molecules were labeled to FBP molecules purified from milk. FA was labeled to horseradish peroxidase (HRP) by EDAC to activate the FA molecules. The pretreated samples or standards were added into the reaction tube with biotin-FBP and FA-horseradish peroxidase (HRP), FA in the sample compete with FA-HRP for binding to biotin-FBP, the signal is inversely proportional to the FA concentration. The method established shows good thermostability and performance. The limitation of detection (LOD) is 0.44 ng/mL. The intra-assay coefficient of variation (CV) is 3.6%–7.1%, the interassay CV is 4.2%–7.5%, and the recovery rate is 92.1%–103.5%. Cross reactivity (CR) was remarkably low with aminopterin, folinic acid, and methotrexate. The method shows good correlation with the FA CLIA product from Beckman Coulter; the equation is y = 0.9618x−0.1434 while the R2 value is 0.9224. The established method is sensitive, rapid, and accurate which can fully satisfy for the clinical requirement.

scholarly journals Simultaneous quantification of cholesterol sulfate, androgen sulfates, and progestagen sulfates in human serum by LC-MS/MS

2015 ◽  
Vol 56 (9) ◽  
pp. 1843-1851 ◽  
Author(s):  
Alberto Sánchez-Guijo ◽  
Vinzenz Oji ◽  
Michaela F. Hartmann ◽  
Heiko Traupe ◽  
Stefan A. Wudy
Keyword(s):  
Human Serum ◽  
Cholesterol Sulfate ◽  
Simultaneous Quantification

Highly Efficient Analysis of Glycoprotein Sialylation in Human Serum by Simultaneous Quantification of Glycosites and Site-Specific Glycoforms

2019 ◽  
Vol 18 (9) ◽  
pp. 3439-3446 ◽  
Author(s):  
Hongqiang Qin ◽  
Xuefang Dong ◽  
Jiawei Mao ◽  
Yao Chen ◽  
Mingming Dong ◽  
...  
Keyword(s):  
Human Serum ◽  
Site Specific ◽  
Simultaneous Quantification ◽  
Highly Efficient
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