scholarly journals Alpha‐ and beta‐tubulin isotypes are differentially expressed during brain development

2020 ◽  
Author(s):  
Torben J. Hausrat ◽  
Jennifer Radwitz ◽  
Franco L. Lombino ◽  
Petra Breiden ◽  
Matthias Kneussel
Keyword(s):  
Brain Development ◽  
Differentially Expressed ◽  
Beta Tubulin ◽  
Tubulin Isotypes

scholarly journals Immunological discrimination of β-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III β-tubulins

1992 ◽  
Vol 288 (3) ◽  
pp. 919-924 ◽  
Author(s):  
I Linhartová ◽  
P Dráber ◽  
E Dráberová ◽  
V Viklický
Keyword(s):  
Mouse Brain ◽  
Specific Class ◽  
Class Iii ◽  
Post Translational Modification ◽  
Beta Tubulin ◽  
Developmentally Regulated ◽  
Post Translational Modifications ◽  
Tubulin Isotypes ◽  
Tubulin Isoforms ◽  
Developing Mouse Brain

Individual beta-tubulin isoforms in developing mouse brain were characterized using immunoblotting, after preceding high-resolution isoelectric focusing, with monoclonal antibodies against different structural regions of beta-tubulin. Some of the antibodies reacted with a limited number of tubulin isoforms in all stages of brain development and in HeLa cells. The epitope for the TU-14 antibody was located in the isotype-defining domain and was present on the beta-tubulin isotypes of classes I, II and IV, but absent on the neuron-specific class-III isotype. The data suggest that non-class-III beta-tubulins in mouse brain are substrates for developmentally regulated post-translational modifications and that beta-tubulins of non-neuronal cells are also post-translationally modified.

Identification of two human beta-tubulin isotypes

1983 ◽  
Vol 3 (5) ◽  
pp. 854-862
Author(s):  
J L Hall ◽  
L Dudley ◽  
P R Dobner ◽  
S A Lewis ◽  
N J Cowan
Keyword(s):  
Cdna Clone ◽  
Hela Cells ◽  
Untranslated Region ◽  
Genomic Sequence ◽  
Beta Tubulin ◽  
Tubulin Isotypes ◽  
Tubulin Genes ◽  
Processed Pseudogene ◽  
Carboxy Terminal ◽  
High Degree

The sequence of a human beta-tubulin cDNA clone (D beta-1) is described; our data revealed 95.6% homology compared with the sequence of a human beta-tubulin processed pseudogene derived by reverse transcription of a processed mRNA (Wilde et al., Nature [London] 297:83-84, 1982). However, the amino acid sequence encoded by this cDNA showed less homology with pig and chicken beta-tubulin sequences than the latter did to each other, with major divergence within the 15 carboxy-terminal amino acids. On the other hand, an independently isolated, functionally expressed genomic human beta-tubulin sequence (5 beta) possessed a very high degree of homology with chicken and pig beta-tubulins in this region. Thus, human cells appear to contain two distinct beta-tubulin isotypes. Both the intact beta-tubulin cDNA clone and a subclone containing only the 3' untranslated region detected two mRNA species in HeLa cells; these mRNAs were 1.8 and 2.6 kilobases long and were present in about equal amounts. Two independently subcloned probes constructed from the 3' untranslated region of the 5 beta genomic sequence also detected a 2.6-kilobase beta-tubulin mRNA. However, the 3'-untranslated-region probes from the cDNA clone and the genomic sequence did not cross-hybridize. Thus, at least two human beta-tubulin genes, each specifying a distinct isotype, are expressed in HeLa cells, and the 2.6-kilobase mRNA band is a composite of at least two comigrating beta-tubulin mRNAs.

scholarly journals Regulation of three beta-tubulin mRNAs during rat brain development.

1985 ◽  
Vol 4 (13B) ◽  
pp. 3667-3673 ◽  
Author(s):  
I. Ginzburg ◽  
A. Teichman ◽  
H.J. Dodemont ◽  
L. Behar ◽  
U.Z. Littauer
Keyword(s):  
Brain Development ◽  
Rat Brain ◽  
Beta Tubulin

Smad2 isoforms are differentially expressed during mouse brain development and aging

2009 ◽  
Vol 27 (5) ◽  
pp. 501-510 ◽  
Author(s):  
Uwe Ueberham ◽  
Peggy Lange ◽  
Elke Ueberham ◽  
Martina K. Brückner ◽  
Maike Hartlage‐Rübsamen ◽  
...  
Keyword(s):  
Brain Development ◽  
Mouse Brain ◽  
Differentially Expressed ◽  
Development And Aging ◽  
Mouse Brain Development

Genetic variants in miRNAs differentially expressed during brain development and their relevance to psychiatric disorders susceptibility

2020 ◽  
pp. 1-12
Author(s):  
Clarice Brinck Brum ◽  
Vanessa Rodrigues Paixão-Côrtes ◽  
Andressa Marques Carvalho ◽  
Thais Martins-Silva ◽  
Marina Xavier Carpena ◽  
...  
Keyword(s):  
Brain Development ◽  
Psychiatric Disorders ◽  
Genetic Variants ◽  
Differentially Expressed

Expression of beta-tubulin isotypes in human primary ovarian carcinoma

2007 ◽  
Vol 105 (3) ◽  
pp. 586-592 ◽  
Author(s):  
Yoshihiro Ohishi ◽  
Yoshinao Oda ◽  
Yuji Basaki ◽  
Hiroaki Kobayashi ◽  
Norio Wake ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Beta Tubulin ◽  
Tubulin Isotypes

scholarly journals Complex regulation and functional versatility of mammalian alpha- and beta-tubulin isotypes during the differentiation of testis and muscle cells.

1988 ◽  
Vol 106 (6) ◽  
pp. 2023-2033 ◽  
Author(s):  
S A Lewis ◽  
N J Cowan
Keyword(s):  
Cell Types ◽  
Beta Tubulin ◽  
Specific Expression ◽  
Alpha Tubulin ◽  
Tubulin Isotypes ◽  
Tubulin Isotype ◽  
A Cell ◽  
Complex Regulation ◽  
Carboxy Terminal ◽  
Different Cell Types

In the accompanying paper (Gu, W., S. A. Lewis, and N. J. Cowan. 1988. J. Cell Biol. 106: 2011-2022), we report the generation of three antisera, each of which uniquely recognizes a different mammalian alpha-tubulin isotype, plus a fourth antibody that distinguishes between microtubules containing the tyrosinated and nontyrosinated form of the only known mammalian alpha-tubulin gene product that lacks an encoded carboxy-terminal tyrosine residue. These sera, together with five sera we raised that distinguish among the known mammalian beta-tubulin isotypes, have been used to study patterns of tubulin isotype-specific expression in muscle and testis, two tissues in which characteristic developmental changes are accompanied by dramatic rearrangements in microtubule structures. As in the case of cells in culture, there is no evidence to suggest that there is subcellular sorting of different tubulin isotypes among different kinds of microtubule, even in a cell type (the developing spermatid) that simultaneously contains such functionally distinct structures as the manchette and the flagellum. On the other hand, the patterns of expression of the various tubulin isotypes show marked and distinctive differences in different cell types and, in at least one case, evidence is presented for regulation at the translational or posttranslational level. The significance of these observations is discussed in terms of the existence of the mammalian alpha- and beta-tubulin multigene families.

scholarly journals Three Rice cDNA Clones Encoding Different [beta]-Tubulin Isotypes

1995 ◽  
Vol 108 (2) ◽  
pp. 823-824 ◽  
Author(s):  
D. Breviario ◽  
S. Giani ◽  
C. Meoni
Keyword(s):  
Cdna Clones ◽  
Beta Tubulin ◽  
Tubulin Isotypes ◽  
Rice Cdna

scholarly journals Cloning of cDNAs for two .BETA.-tubulin isotypes expressed in murine T cell lymphoma, L5178Y and analysis of their translation products.

1987 ◽  
Vol 12 (4) ◽  
pp. 317-325 ◽  
Author(s):  
Fumio Matsuzaki ◽  
Fumiko Harada ◽  
Yo-ichi Nabeshima ◽  
Yoshiaki Fujii-Kuriyama ◽  
Ichiro Yahara
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Beta Tubulin ◽  
Tubulin Isotypes
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