Examination of Age-dependent effects of fetal ethanol exposure on behavior, hippocampal cell counts, and doublecortin immunoreactivity in rats

2013 ◽  
Vol 74 (5) ◽  
pp. 498-513 ◽  
Author(s):  
Birsen Elibol-Can ◽  
Ilknur Dursun ◽  
Ilknur Telkes ◽  
Ertugrul Kilic ◽  
Sinan Canan ◽  
...  
Keyword(s):  
Ethanol Exposure ◽  
Cell Counts ◽  
Hippocampal Cell ◽  
Age Dependent ◽  
Fetal Ethanol

scholarly journals Role of zinc insufficiency in fetal alveolar macrophage dysfunction and RSV exacerbation associated with fetal ethanol exposure

Alcohol ◽  
2019 ◽  
Vol 80 ◽  
pp. 5-16 ◽  
Author(s):  
Juna Konomi Johnson ◽  
Frank L. Harris ◽  
Xiao-Du Ping ◽  
Theresa W. Gauthier ◽  
Lou Ann S. Brown
Keyword(s):  
Alveolar Macrophage ◽  
Ethanol Exposure ◽  
Macrophage Dysfunction ◽  
Fetal Ethanol

Effects of In Vitro Ethanol and Fetal Ethanol Exposure on Glutathione Stimulation of N-Methyl-D-Aspartate Receptor Function

1993 ◽  
Vol 17 (3) ◽  
pp. 643-650 ◽  
Author(s):  
Melanie S. Weaver ◽  
Young-Hee Lee ◽  
Jimmy L. Morris ◽  
Patrick K. Randall ◽  
Timothy Schallert ◽  
...  
Keyword(s):  
Ethanol Exposure ◽  
Receptor Function ◽  
Aspartate Receptor ◽  
Fetal Ethanol ◽  
Stimulation Of

Transgene Dose- and Age-Dependent Development of Myeloproliferative Neoplasm Phenotypes In JAK2V617F Transgene Mice

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 1980-1980
Author(s):  
Wanting Ho ◽  
Wanming Zhao ◽  
Zhizhuang Joe Zhao
Keyword(s):  
Transgenic Mice ◽  
Copy Number ◽  
Conflicts Of Interest ◽  
Myeloproliferative Neoplasms ◽  
Gene Copy ◽  
Cell Transplant ◽  
Dependent Manner ◽  
Hematopoietic Stem ◽  
Cell Counts ◽  
Age Dependent

Abstract Abstract 1980 Myeloproliferative neoplasms (MPNs) are heterogeneous hematologic disorders represented by three main phenotypes: polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). The major molecular lesion in these diseases is JAK2V617F, which occurs in over 95% patients with PV and in over 50% of patients with ET or PMF. The pathogenic effects of JAK2V617F have been demonstrated by retrovirus-mediated gene transfer, transgenic, and knock-in mouse models, but the precise mode of JAK2V617F action is not clear. Interestingly, in the knock-in model, expression of JAK2V617F causes severe PV-like disease but not ET-like phenotype as seen in patients. To verify the pathogenic role of JAK2V617F, we further characterized the phenotypes of three lines of JAK2V617F transgenic mice generated by using the vav gene promoter which drives expression of transgenes in the hematopoietic system. These mice developed MPN-like phenotypes in a transgene dose- and age-dependent manner. Line A mice have a JAK2V617F gene copy number of 13; they develop MPN phenotype with marked increases in blood counts and enlarged spleens as early as 4–6 weeks after birth. In contrast, lines B and D mice have a transgene copy number of 2 and 1, respectively, and it takes nearly 70 weeks for these mice to show MPN-like phenotypes. The phenotype of line A mice is particularly noteworthy. Essentially all the hemizygous line A mice displayed an ET-like phenotype with marked elevations in platelet counts (usually over 4000×109/L by the age of 15 weeks), but only a slight increase in red cell and white cell counts. In contrast, all the homozygous mice exhibited a clear PV-like phenotype with elevations in all three types of blood cells, although their platelets hardly ever went over 4000×109/L. The hemizygous mice developed myelofibrosis after 30 weeks while the homozygous mice showed the symptom within only 10 weeks. As expected, the increased blood cell counts and formation of myelofibrosis are associated with mobilization of hematopoietic stem/progenitor cells to peripheral hematopoietic tissues (blood, spleen, and liver). By conducting stem cell transplant experiments, we further proved that JAK2V617F-induced ET and PV-like phenotypes are transplantable. Our study demonstrates that transgenic expression of JAK2V617F is capable of producing all three phenotypes of MPNs in a transgense dose- and age-dependent manner. Our transgenic mice thus represent an excellent model system to study MPNs. Disclosures: No relevant conflicts of interest to declare.

Age-dependent effect of prenatal stress on hippocampal cell proliferation in female rats

2009 ◽  
Vol 29 (3) ◽  
pp. 635-640 ◽  
Author(s):  
Muriel Koehl ◽  
Valerie Lemaire ◽  
Michel Le Moal ◽  
Djoher N. Abrous
Keyword(s):  
Cell Proliferation ◽  
Prenatal Stress ◽  
Female Rats ◽  
Dependent Effect ◽  
Hippocampal Cell ◽  
Age Dependent
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