Migration and ramification of microglia in quail embryo retina organotypic cultures

2011 ◽  
Vol 71 (4) ◽  
pp. 296-315 ◽  
Author(s):  
Maria-Carmen Carrasco ◽  
Julio Navascués ◽  
Miguel A. Cuadros ◽  
Ruth Calvente ◽  
David Martín-Oliva ◽  
...  
Keyword(s):  
Organotypic Cultures ◽  
Quail Embryo

Epithelial Organotypic Cultures: A Viable Model to Address Mechanisms of Carcinogenesis by Epitheliotropic Viruses

2018 ◽  
Vol 18 (4) ◽  
pp. 246-255 ◽  
Author(s):  
Lara Termini ◽  
Enrique Boccardo
Keyword(s):  
Three Dimensional ◽  
Cell Types ◽  
Experimental Models ◽  
Biological Research ◽  
Specific Gene ◽  
Specific Cell ◽  
Organotypic Cultures ◽  
Skin Physiology

In vitro culture of primary or established cell lines is one of the leading techniques in many areas of basic biological research. The use of pure or highly enriched cultures of specific cell types obtained from different tissues and genetics backgrounds has greatly contributed to our current understanding of normal and pathological cellular processes. Cells in culture are easily propagated generating an almost endless source of material for experimentation. Besides, they can be manipulated to achieve gene silencing, gene overexpression and genome editing turning possible the dissection of specific gene functions and signaling pathways. However, monolayer and suspension cultures of cells do not reproduce the cell type diversity, cell-cell contacts, cell-matrix interactions and differentiation pathways typical of the three-dimensional environment of tissues and organs from where they were originated. Therefore, different experimental animal models have been developed and applied to address these and other complex issues in vivo. However, these systems are costly and time consuming. Most importantly the use of animals in scientific research poses moral and ethical concerns facing a steadily increasing opposition from different sectors of the society. Therefore, there is an urgent need for the development of alternative in vitro experimental models that accurately reproduce the events observed in vivo to reduce the use of animals. Organotypic cultures combine the flexibility of traditional culture systems with the possibility of culturing different cell types in a 3D environment that reproduces both the structure and the physiology of the parental organ. Here we present a summarized description of the use of epithelial organotypic for the study of skin physiology, human papillomavirus biology and associated tumorigenesis.

scholarly journals Cytokine inflammatory threat, but not LPS one, shortens GABAergic synaptic currents in the mouse spinal cord organotypic cultures

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Vincenzo Giacco ◽  
Giulia Panattoni ◽  
Manuela Medelin ◽  
Elena Bonechi ◽  
Alessandra Aldinucci ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Organotypic Cultures ◽  
Synaptic Currents ◽  
Mouse Spinal Cord

Neurotoxicity of Seven MEIC Chemicals Evaluated in Organotypic Cultures of Chick Embryonic Dorsal Root Ganglia

1997 ◽  
Vol 25 (3) ◽  
pp. 303-309
Author(s):  
Václav Mandys ◽  
Katerina Jirsová ◽  
Jirí Vrana
Keyword(s):  
Toxic Effect ◽  
Neurite Outgrowth ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Growth Parameters ◽  
In Vitro Cytotoxicity ◽  
Organotypic Cultures ◽  
Response Curves ◽  
Agar Culture

The neurotoxic effects of seven selected Multicenter Evaluation of In Vitro Cytotoxicity programme chemicals (methanol, ethanol, isopropanol, sodium chloride, potassium chloride, iron [II] sulphate and chloroform) were evaluated in organotypic cultures of chick embryonic dorsal root ganglia (DRG), maintained in a soft agar culture medium. Two growth parameters of neurite outgrowth from the ganglia — the mean radial length of neurites and the area of neurite outgrowth — were used to evaluate the toxicities of the chemicals. Dose-dependent decreases of both parameters were observed in all experiments. IC50 values (the concentration causing 50% inhibition of growth) were calculated from the dose-response curves established at three time-points during culture, i.e. 24, 48 and 72 hours. The lowest toxic effect was observed in cultures exposed to methanol (the IC50 ranging from 580mM to 1020mM). The highest toxic effect was observed in cultures exposed to iron (II) sulphate (the IC50 ranging from 1.2mM to 1.7mM). The results of other recent experiments suggest that organotypic cultures of DRG can be used during in vitro studies on target organ toxicity within the peripheral nervous system. Moreover, these cultures preserve the internal organisation of the tissue, maintain intercellular contacts, and thus reflect the in vitro situation, more precisely than other cell cultures.

scholarly journals Epigenetic effects of chromatin remodeling agents on organotypic cultures

Epigenomics ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 341-358 ◽  
Author(s):  
Silvia M Sirchia ◽  
Alice Faversani ◽  
Davide Rovina ◽  
Maria V Russo ◽  
Leda Paganini ◽  
...  
Keyword(s):  
Chromatin Remodeling ◽  
Organotypic Cultures ◽  
Epigenetic Effects

Chemical neurotoxicity: Detection and analysis in organotypic cultures of sensory and motor systems

1986 ◽  
Vol 24 (6-7) ◽  
pp. 539-544 ◽  
Author(s):  
P.S. Spencer ◽  
S.M. Crain ◽  
M.B. Bornstein ◽  
E.R. Peterson ◽  
T. Van De Water
Keyword(s):  
Organotypic Cultures ◽  
Motor Systems

Effects of retinoid deficiency on the development of the heart and vascular system of the quail embryo

1986 ◽  
Vol 50 (1) ◽  
pp. 135-152 ◽  
Author(s):  
U. I. Heine ◽  
A. B. Roberts ◽  
E. F. Munoz ◽  
N. S. Roche ◽  
M. B. Sporn
Keyword(s):  
Vascular System ◽  
Quail Embryo

Expression of the proenkephalin A gene in organotypic cultures of neocortex from newborn rats

1996 ◽  
Vol 35 (1-2) ◽  
pp. 157-163 ◽  
Author(s):  
Lothar Just ◽  
Claudia Olenik ◽  
Bernd Heimrich ◽  
Dieter K. Meyer
Keyword(s):  
Newborn Rats ◽  
Organotypic Cultures ◽  
Proenkephalin A
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