Electrophysiological analysis of exogenous and endogenous adenosine actions in the rat and human hippocampus in vitro

1993 ◽  
Vol 28 (3) ◽  
pp. 386-389 ◽  
Author(s):  
Helmut L. Haas ◽  
Urs Gerber ◽  
Robert W. Greene ◽  
David R. Stevens
Keyword(s):  
Endogenous Adenosine ◽  
Human Hippocampus ◽  
Electrophysiological Analysis

Formation of Glutamate and GABA in Epileptogenic Tissue from Human Hippocampus in Vitro

1976 ◽  
pp. 111-118 ◽  
Author(s):  
T. Turský ◽  
M. Laššánová ◽  
M. Šramka ◽  
P. Nádvorník
Keyword(s):  
Human Hippocampus

IN VITRO WHOLE BRAIN PREPARATION OF FISH FOR THE ELECTROPHYSIOLOGICAL ANALYSIS OF SENSORY PATHWAYS

Bioacoustics ◽  
2002 ◽  
Vol 12 (2-3) ◽  
pp. 328-330 ◽  
Author(s):  
MICHAELA MEYER ◽  
DENNIS T.T. PLACHTA ◽  
ARTHUR N. POPPER ◽  
HORST BLECKMANN
Keyword(s):  
Whole Brain ◽  
Sensory Pathways ◽  
Electrophysiological Analysis

Inkjet-printed PEDOT:PSS multi-electrode arrays for low-cost in vitro electrophysiology

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3776-3786 ◽  
Author(s):  
Leonardo D. Garma ◽  
Laura M. Ferrari ◽  
Paola Scognamiglio ◽  
Francesco Greco ◽  
Francesca Santoro
Keyword(s):  
Cell Cultures ◽  
Low Cost ◽  
Fabrication Process ◽  
Cardiac Cell ◽  
Proof Of Concept ◽  
Electrode Arrays ◽  
Electrophysiological Analysis ◽  
Valid Proof ◽  
Cardiac Cell Cultures

We present an innovative fabrication process for the production of low cost fully-plastic flexible MEAs and prove that they are a valid proof-of-concept for a platform for the electrophysiological analysis of cardiac cell cultures.

Activity-dependent feedback modulation of spike patterning of supraoptic nucleus neurons by endogenous adenosine

2006 ◽  
Vol 291 (1) ◽  
pp. R83-R90 ◽  
Author(s):  
P. M. Bull ◽  
C. H. Brown ◽  
J. A. Russell ◽  
M. Ludwig
Keyword(s):  
Electrical Activity ◽  
Hazard Function ◽  
Supraoptic Nucleus ◽  
Neuronal Excitability ◽  
Cell Activity ◽  
Male Rats ◽  
Firing Rates ◽  
Endogenous Adenosine

Neuropeptide secretion from the dendrites of hypothalamic magnocellular supraoptic nucleus (SON) neurons contributes to the regulation of neuronal activity patterning, which ultimately determines their peptide output from axon terminals in the posterior pituitary gland. SON dendrites also secrete a number of other neuromodulators, including ATP. ATP degrades to adenosine in the extracellular space to complement transported adenosine acting on pre- and postsynaptic SON A1 receptors to reduce neuronal excitability, measured in vitro. To assess adenosine control of electrical activity in vivo, we made extracellular single-unit recordings of the electrical activity of SON neurons in anesthetized male rats. Microdialysis application (retrodialysis) of the A1 receptor antagonist, 8-cyclopentyl-1,3-dimethylxanthine (CPT) increased phasic vasopressin cell intraburst firing rates progressively over the first 5 s by 4.5 ± 1.6 Hz ( P < 0.05), and increased burst duration by 293 ± 64% ( P < 0.05). Hazard function plots were generated from interval interspike histograms and revealed that these effects were associated with increased postspike excitability. In contrast, CPT had no effect on the firing rates and hazard function plot profiles of continuously active vasopressin and oxytocin cells. However, CPT significantly increased clustering of spikes, as quantified by the index of dispersion, in oxytocin cells and continuously active vasopressin cells (by 267 ± 113% and 462 ± 67%, respectively, P < 0.05). Indeed, in 4 of 5 continuously active vasopressin cells, CPT induced a pseudophasic activity pattern. Together, these results indicate that endogenous adenosine is involved in the local control of SON cell activity in vivo.

scholarly journals Electrophysiological analysis of healthy and dystrophic 3D bioengineered skeletal muscle tissues

2021 ◽  
Author(s):  
Christine T. Nguyen ◽  
Majid Ebrahimi ◽  
Penney M. Gilbert ◽  
Bryan Andrew Stewart
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cell ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Dystrophin Gene ◽  
Muscle System ◽  
Electrophysiological Analysis ◽  
Cytoskeletal Network ◽  
Muscle Tissues

Recently, methods for creating three-dimensional (3D) human skeletal muscle tissues from myogenic cell lines have been reported. Bioengineered muscle tissues are contractile and respond to electrical and chemical stimulation. In this study we provide an electrophysiological analysis of healthy and dystrophic 3D bioengineered skeletal muscle tissues. We focus on Duchenne muscular dystrophy (DMD), a fatal muscle disorder involving the skeletal muscle system. The dystrophin gene, which when mutated causes DMD, encodes for the Dystrophin protein, which anchors the cytoskeletal network inside of a muscle cell to the extracellular matrix outside the cell. Here, we enlist a 3D in vitro model of DMD muscle tissue, to evaluate an understudied aspect of DMD, muscle cell electrical properties uncoupled from presynaptic neural inputs. Our data shows that electrophysiological aspects of DMD are replicated in the 3D bioengineered skeletal muscle tissue model. Furthermore, we test a block co-polymer, poloxamer 188, and demonstrate capacity for improving the membrane potential in DMD muscle. Therefore, this study serves as the baseline for a new in vitro method to examine potential therapies directed at muscular disorders.

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