Inkjet-printed PEDOT:PSS multi-electrode arrays for low-cost in vitro electrophysiology

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3776-3786 ◽  
Author(s):  
Leonardo D. Garma ◽  
Laura M. Ferrari ◽  
Paola Scognamiglio ◽  
Francesco Greco ◽  
Francesca Santoro
Keyword(s):  
Cell Cultures ◽  
Low Cost ◽  
Fabrication Process ◽  
Cardiac Cell ◽  
Proof Of Concept ◽  
Electrode Arrays ◽  
Electrophysiological Analysis ◽  
Valid Proof ◽  
Cardiac Cell Cultures

We present an innovative fabrication process for the production of low cost fully-plastic flexible MEAs and prove that they are a valid proof-of-concept for a platform for the electrophysiological analysis of cardiac cell cultures.

Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures

2020 ◽  
Vol 25 (3) ◽  
pp. 234-246
Author(s):  
Charles McRae White ◽  
Mark A. Haidekker ◽  
William S. Kisaalita
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Cell Biology ◽  
Low Cost ◽  
3D Cell Culture ◽  
Biomechanical Properties ◽  
Practical Applications ◽  
3D Cell Cultures

New insights into the biomechanical properties of cells are revealing the importance of these properties and how they relate to underlying molecular, architectural, and behavioral changes associated with cell state and disease processes. However, the current understanding of how these in vitro biomechanical properties are associated with in vivo processes has been developed based on the traditional monolayer (two-dimensional [2D]) cell culture, which traditionally has not translated well to the three-dimensional (3D) cell culture and in vivo function. Many gold standard methods and tools used to observe the biomechanical properties of 2D cell cultures cannot be used with 3D cell cultures. Fluorescent molecules can respond to external factors almost instantaneously and require relatively low-cost instrumentation. In this review, we provide the background on fluorescent molecular rotors, which are attractive tools due to the relationship of their emission quantum yield with environmental microviscosity. We make the case for their use in both 2D and 3D cell cultures and speculate on their fundamental and practical applications in cell biology.

scholarly journals Mechanisms of Intrinsic Beating Variability in Cardiac Cell Cultures and Model Pacemaker Networks

2007 ◽  
Vol 92 (10) ◽  
pp. 3734-3752 ◽  
Author(s):  
Julien G.C. Ponard ◽  
Aleksandar A. Kondratyev ◽  
Jan P. Kucera
Keyword(s):  
Cell Cultures ◽  
Cardiac Cell ◽  
Cardiac Cell Cultures

Myosin heavy chain expression in embryonic cardiac cell cultures

1986 ◽  
Vol 115 (1) ◽  
pp. 204-214 ◽  
Author(s):  
B.J. Zadeh ◽  
A. González-Sánchez ◽  
D.A. Fischman ◽  
D.M. Bader
Keyword(s):  
Myosin Heavy Chain ◽  
Cell Cultures ◽  
Heavy Chain ◽  
Cardiac Cell ◽  
Cardiac Cell Cultures

scholarly journals A low-cost method to test cytotoxic effects of Crotalus vegrandis (Serpentes: Viperidae) venom on kidney cell cultures

2005 ◽  
Vol 47 (3) ◽  
pp. 147-152 ◽  
Author(s):  
María E. Girón ◽  
Irma Aguilar ◽  
Lisandro Romero ◽  
Elda E. Sánchez ◽  
John c. Pérez ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Renal Cortex ◽  
Smooth Muscle Actin ◽  
Low Cost ◽  
Colorimetric Method ◽  
Vero Cells ◽  
Renal Lesion ◽  
Crude Venom

The pathogenesis of the renal lesion upon envenomation by snakebite has been related to myolysis, hemolysis, hypotension and/or direct venom nephrotoxicity caused by the venom. Both primary and continuous cell culture systems provide an in vitro alternative for quantitative evaluation of the toxicity of snake venoms. Crude Crotalus vegrandis venom was fractionated by molecular exclusion chromatography. The toxicity of C. vegrandis crude venom, hemorrhagic, and neurotoxic fractions were evaluated on mouse primary renal cells and a continuous cell line of Vero cells maintained in vitro. Cells were isolated from murine renal cortex and were grown in 96 well plates with Dulbecco's Modified Essential Medium (DMEM) and challenged with crude and venom fractions. The murine renal cortex cells exhibited epithelial morphology and the majority showed smooth muscle actin determined by immune-staining. The cytotoxicity was evaluated by the tetrazolium colorimetric method. Cell viability was less for crude venom, followed by the hemorrhagic and neurotoxic fractions with a CT50 of 4.93, 18.41 and 50.22 µg/mL, respectively. The Vero cell cultures seemed to be more sensitive with a CT50 of 2.9 and 1.4 µg/mL for crude venom and the hemorrhagic peak, respectively. The results of this study show the potential of using cell culture system to evaluate venom toxicity.

Energy metabolism of cardiac cell cultures during oxygen deprivation: effects of creatine and arachidonic acid

1985 ◽  
Vol 34 (1) ◽  
pp. 145-147 ◽  
Author(s):  
Elisabeth Millanvoye-Van Brussel ◽  
Marianne Freyss-Beguin ◽  
Geneviéve Griffaton ◽  
Paul Lechat
Keyword(s):  
Arachidonic Acid ◽  
Energy Metabolism ◽  
Cell Cultures ◽  
Oxygen Deprivation ◽  
Cardiac Cell ◽  
Cardiac Cell Cultures
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