Flow cytometric analysis of fine needle aspirates is affected by tumor subtype, but not by anatomic location nor technique

2020 ◽  
Vol 48 (6) ◽  
pp. 538-546
Author(s):  
Laura M. Wake ◽  
Christopher J. VandenBussche ◽  
Syed Z. Ali ◽  
Nina D. Wagner‐Johnston ◽  
Kathleen H. Burns ◽  
...  
Keyword(s):  
Flow Cytometric Analysis ◽  
Tumor Subtype ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Flow Cytometric ◽  
Anatomic Location

scholarly journals The Accuracy of Combined Cytopathologic and Flow Cytometric Analysis of Fine-Needle Aspirates of Lymph Nodes

2000 ◽  
Vol 114 (1) ◽  
pp. 18-28 ◽  
Author(s):  
Theresa L. Nicol ◽  
Mark Silberman ◽  
Dorothy L. Rosenthal ◽  
Michael J. Borowitz
Keyword(s):  
Lymph Nodes ◽  
Flow Cytometric Analysis ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Flow Cytometric

Comparative study of interphase cytogenetics, flow cytometric analysis, and nuclear grade of fine-needle aspirates of breast carcinoma

1994 ◽  
Vol 11 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Ricardo S. Cajulis ◽  
Sophia Kotliar ◽  
George K. Haines ◽  
Denise Frias-Hidvegi ◽  
Maurice O'Gorman
Keyword(s):  
Breast Carcinoma ◽  
Comparative Study ◽  
Flow Cytometric Analysis ◽  
Nuclear Grade ◽  
Interphase Cytogenetics ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Flow Cytometric

FLOW CYTOMETRIC DNA CONTENT IN FINE NEEDLE ASPIRATES OF HEPATOCELLULAR CARCINOMA TO ASSESS PROGNOSIS

1991 ◽  
Vol 73 (2-3) ◽  
pp. 42a-42a
Author(s):  
Michèle Perrin-Cottier ◽  
Claude Jouffre ◽  
Odile Sabido ◽  
Ivan Maubon ◽  
Olivier Dumas ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Content ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Flow Cytometric

Clinical Impact of Endoscopic Ultrasound-Guided Fine Needle Aspiration Biopsy In the Diagnosis of Patients with Lymphoma Who Have No Superficial Lymphadenopathies

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 5083-5083
Author(s):  
Naoe Goto ◽  
Ichiro Yasuda ◽  
Hisashi Tsurumi ◽  
Shinpei Doi ◽  
Takeshi Hara ◽  
...  
Keyword(s):  
Endoscopic Ultrasound ◽  
Fine Needle Aspiration ◽  
Conflicts Of Interest ◽  
Flow Cytometric Analysis ◽  
Needle Aspiration ◽  
Chromosomal Analysis ◽  
Poor Performance Status ◽  
Ultrasound Guided ◽  
Fine Needle ◽  
Flow Cytometric

Abstract Abstract 5083 Background: In patients with mediastinal and intra-abdominal lymphoma who do not have superficial lymphadenopathies, the diagnosis is usually made by surgical sampling with open thoracic surgery, laparotomy, mediastinoscopy, thoracoscopy, or laparoscopy. However, these surgical procedures are cumbersome and invasive; therefore, the treatment is often delayed, and doctors often hesitate to attempt the procedures for patients in poor performance status (PS). Recently, endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) has been used for the diagnosis of unknown mediastinal and abdominal lymphadenopathies or masses. With this procedure, pathological materials can be obtained through the gastrointestinal tract under real-time ultrasound imaging guidance. It is regarded as a minimally invasive, safe, and relatively accurate procedure. However, only a small amount of material can be sampled with this technique. Immunohistochemical, flow cytometric, and chromosomal analyses are essential for the diagnosis of lymphoma, but it is not clear whether these analyses can be assessed on samples obtained by EUS-FNA. Aim: To evaluate the yield of EUS-FNA for the diagnosis of lymphoma. Patients and Methods: Between June 2005 and December 2009, a total of 224 consecutive patients who were suspected to have lymphoma underwent EUS-FNA with a 19-gauge needle at our hospital. We reviewed the baseline characteristics of the patients; the histological diagnosis including immunohistochemical stainings, flow cytometric analysis (FCM), and chromosomal analysis (by the G-banding method) of the FNA samples; and the final diagnosis. EUS-FNA was performed in 241 lesions in the 224 patients. One hundred and forty-three patients (63.8%) underwent the procedure as outpatients. Ten in 224 patients had a poor PS (grade 4). Results: The locations of the lesions were mediastinal in 99 and intra-abdominal in 142 (inclusive of 9 splenic, 5 left adrenal, 2 pancreatic, and 1 right renal lesions). After all, 142 in 224 patients were finally diagnosed as having lymphoma. Among them, 138 in 142 patients (97.2%) were diagnosed by EUS-FNA, and detailed classification in accordance with the WHO system was also possible in 125 in 142 patients (88.0%) on the basis of comprehensive findings including immunohistochemical staining, FCM, FISH method, and chromosomal analysis. While, remaining 82 in 224 patients were diagnosed as having inflammatory diseases such as sarcoidosis, necrotizing lymphadenitis, tuberculosis, or other cancers. No serious complications occurred with the procedure. Conclusions: EUS-FNA is a safe and convenient procedure with high diagnostic value for the diagnosis of lymphoma. Therefore, this procedure should be initially considered to attempt for patients with mediastinal and intra-abdomnal lymphadenopathy or mass who do have no superficial lesions. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Flow Cytometric Immunophenotypic Assessment of T-Cell Clonality by VβRepertoire Analysis in Fine-Needle Aspirates and Cerebrospinal Fluid

2012 ◽  
Vol 137 (2) ◽  
pp. 220-226 ◽  
Author(s):  
Prashant Tembhare ◽  
Constance M. Yuan ◽  
John C. Morris ◽  
John E. Janik ◽  
Armando C. Filie ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
T Cell ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Flow Cytometric ◽  
T Cell Clonality ◽  
Cell Clonality

scholarly journals Endoscopic ultrasound and endobronchial ultrasound-guided fine-needle aspiration of deep-seated lymphadenopathy: Analysis of 1338 cases

CytoJournal ◽  
2012 ◽  
Vol 9 ◽  
pp. 14 ◽  
Author(s):  
Amberly L Nunez ◽  
Nirag C Jhala ◽  
Andrew J Carroll ◽  
Fady M Mikhail ◽  
Vishnu V.B. Reddy ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Fine Needle Aspiration ◽  
Flow Cytometric Analysis ◽  
Needle Aspiration ◽  
Endobronchial Ultrasound ◽  
Ultrasound Guided ◽  
Fine Needle ◽  
Flow Cytometric

Background: We retrospectively studied 1338 samples of lymph nodes obtained by endoscopic and endobronchial ultrasound-guided fine needle aspiration biopsy (EUS and EBUS-FNAB) with an objective of characterizing the utility of this diagnostic modality in the assessment of deep-seated lymphadenopathy. The secondary aims were to establish the utility in the diagnosis of lymphoma and to determine the number of passes required to obtain adequate cellularity for flow cytometric analysis. Materials and Methods: On-site assessment was performed by a cytopathologist using Diff-Quik (American Scientific Products, McGraw Park, IL) stain. In addition, Papanicolaou and immunohistochemical stains were performed and additional samples were sent for flow cytometric analyses (n = 145). The final cytologic diagnosis was correlated with surgical pathology diagnosis and/or clinical follow-up. In select cases, fluorescence in situ hybridization analysis with specific probes was performed on Diff-Quik smears. Results: Both morphology as well as ancillary studies (flow cytometry or immunohistochemical stain and/or fluorescence in situ hybridization) show that EUS and EBUS-FNA are effective techniques to detect and stage intrathoracic and intra-abdominal tumors. Operating characteristics show that these are highly sensitive (89%) and specific (100%) techniques for the diagnosis of lymphoma. At least two passes provided an average of 5.66 million cells (range, 0.12-62.32 million) for lymphoma cases. Conclusions: EUS and EBUS-FNA are powerful modalities to stage malignancies and at least two passes can provide adequate cells for flow cytometric analysis. We also demonstrate that fluorescence in situ hybridization analysis can be performed on Diff-Quik-stained and mounted smears.

scholarly journals How we assess adequacy of fine-needle aspiration materials intended for flow cytometric analysis

2011 ◽  
Vol 4 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Mohamed Brahimi ◽  
Abdessamad Arabi ◽  
Badra Enta Soltan ◽  
Soufi Osmani ◽  
Hanane Benradouane ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Flow Cytometric Analysis ◽  
Needle Aspiration ◽  
Fine Needle ◽  
Flow Cytometric
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