scholarly journals Flow Cytometric Immunophenotypic Assessment of T-Cell Clonality by VβRepertoire Analysis in Fine-Needle Aspirates and Cerebrospinal Fluid

2012 ◽  
Vol 137 (2) ◽  
pp. 220-226 ◽  
Author(s):  
Prashant Tembhare ◽  
Constance M. Yuan ◽  
John C. Morris ◽  
John E. Janik ◽  
Armando C. Filie ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
T Cell ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Flow Cytometric ◽  
T Cell Clonality ◽  
Cell Clonality

scholarly journals Utility of a Simple and Robust Flow Cytometry Assay for Rapid Clonality Testing in Mature Peripheral T-Cell Lymphomas

2019 ◽  
Vol 151 (5) ◽  
pp. 494-503 ◽  
Author(s):  
Natasha D Novikov ◽  
Gabriel K Griffin ◽  
Graham Dudley ◽  
Mai Drew ◽  
Vanesa Rojas-Rudilla ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Peripheral Blood ◽  
T Cell Clones ◽  
Flow Cytometric ◽  
T Cell Lymphomas ◽  
Cell Clones ◽  
T Cell Clonality ◽  
Cell Clonality ◽  
Peripheral T Cell Lymphomas

AbstractObjectivesFlow cytometry immunophenotyping is limited by poor resolution of T-cell clones. A newly described antibody was recently used to distinguish normal peripheral blood T cells from malignant T-cell clones. Here, we evaluate this antibody as a new diagnostic tool for detecting T-cell clonality in mature peripheral T-cell lymphomas.MethodsImmunostaining for the T-cell receptor β chain constant region 1 (TRBC1) along with routine T-cell markers was performed on 51 peripheral blood and two bone marrow samples submitted to the flow cytometry laboratory for suspected T-cell malignancy.ResultsTRBC immunophenotyping identified malignant T-cell clones with 97% sensitivity and 91% specificity. Findings correlated with molecular T-cell clonality testing. In cases with equivocal molecular results, TRBC1 immunophenotyping provided additional diagnostic information.ConclusionsTRBC1 flow cytometric immunophenotyping is a robust and inexpensive method for identifying T-cell clonality that could easily be incorporated into routine flow cytometric practice.

scholarly journals Flow-Cytometric Assessment of T-Cell Clonality in Clinical Specimens

2007 ◽  
Vol 38 (8) ◽  
pp. 477-482 ◽  
Author(s):  
Ying Li ◽  
Raul C. Braylan ◽  
Samer Z. Al-Quran
Keyword(s):  
T Cell ◽  
Clinical Specimens ◽  
Flow Cytometric ◽  
T Cell Clonality ◽  
Cell Clonality

scholarly journals Flow Cytometric Immunophenotypic Assessment of T-Cell Clonality by VβRepertoire Analysis

2011 ◽  
Vol 135 (6) ◽  
pp. 890-900 ◽  
Author(s):  
Prashant Tembhare ◽  
Constance M. Yuan ◽  
Liqiang Xi ◽  
John C. Morris ◽  
David Liewehr ◽  
...  
Keyword(s):  
T Cell ◽  
Flow Cytometric ◽  
T Cell Clonality ◽  
Cell Clonality

scholarly journals High-Sensitive TRBC1-Based Flow Cytometric Assessment of T-Cell Clonality in Tαβ-Large Granular Lymphocytic Leukemia

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 408
Author(s):  
Noemí Muñoz-García ◽  
F. Morán-Plata ◽  
Neus Villamor ◽  
Margarida Lima ◽  
Susana Barrena ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Receptor ◽  
Lymphocytic Leukemia ◽  
Effector Memory ◽  
Large Granular Lymphocytic Leukemia ◽  
Cell Counts ◽  
Flow Cytometric ◽  
Fcm Analysis ◽  
T Cell Clonality ◽  
Cell Clonality

Flow cytometric (FCM) analysis of the constant region 1 of the T-cell receptor β chain (TRBC1) expression for assessing Tαβ-cell clonality has been recently validated. However, its utility for the diagnosis of clonality of T-large granular lymphocytic leukemia (T-LGLL) needs to be confirmed, since more mature Tαβ cells (i.e., T-LGL normal-counterpart) show broader TRBC1+/TRBC1− ratios vs. total Tαβ cells. We compared the distribution and absolute counts of TRBC1+ and TRBC1− Tαβ-LGL in blood containing polyclonal (n = 25) vs. clonal (n = 29) LGL. Overall, polyclonal TRBC1+ or TRBC1− Tαβ-LGL ranged between 0.36 and 571 cells/μL (3.2–91% TRBC1+ cells), whereas the clonal LGL cases showed between 51 and 11,678 cells/μL (<0.9% or >96% TRBC1+ cells). Among the distinct TCRVβ families, the CD28− effector-memory and terminal-effector polyclonal Tαβ cells ranged between 0 and 25 TRBC1+ or TRBC1− cells/μL and between 0 and 100% TRBC1+ cells, while clonal LGL ranged between 32 and 5515 TRBC1+ or TRBC1− cells/μL, representing <1.6% or >98% TRBC1+ cells. Our data support the utility of the TRBC1-FCM assay for detecting T-cell clonality in expansions of Tαβ-LGL suspected of T-LGLL based on altered percentages of TRBC1+ Tαβ cells. However, in the absence of lymphocytosis or in the case of TαβCD4-LGL expansion, the detection of increased absolute cell counts by the TRBC1-FCM assay for more accurately defined subpopulations of Tαβ-LGL-expressing individual TCRVβ families, allows the detection of T-cell clonality, even in the absence of phenotypic aberrations.

scholarly journals Anti-TRBC1 Antibody-Based Flow Cytometric Detection of T-Cell Clonality: Standardization of Sample Preparation and Diagnostic Implementation

Cancers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 4379
Author(s):  
Noemí Muñoz-García ◽  
Margarida Lima ◽  
Neus Villamor ◽  
F. Javier Morán-Plata ◽  
Susana Barrena ◽  
...  
Keyword(s):  
T Cell ◽  
Residual Disease ◽  
High Specificity ◽  
Accurate Method ◽  
Cell Receptor ◽  
Flow Cytometric ◽  
Useful Flow ◽  
Constant Domains ◽  
T Cell Clonality ◽  
Cell Clonality

A single antibody (anti-TRBC1; JOVI-1 antibody clone) against one of the two mutually exclusive T-cell receptor β-chain constant domains was identified as a potentially useful flow-cytometry (FCM) marker to assess Tαβ-cell clonality. We optimized the TRBC1-FCM approach for detecting clonal Tαβ-cells and validated the method in 211 normal, reactive and pathological samples. TRBC1 labeling significantly improved in the presence of CD3. Purified TRBC1+ and TRBC1− monoclonal and polyclonal Tαβ-cells rearranged TRBJ1 in 44/47 (94%) and TRBJ1+TRBJ2 in 48 of 48 (100%) populations, respectively, which confirmed the high specificity of this assay. Additionally, TRBC1+/TRBC1− ratios within different Tαβ-cell subsets are provided as reference for polyclonal cells, among which a bimodal pattern of TRBC1-expression profile was found for all TCRVβ families, whereas highly-variable TRBC1+/TRBC1− ratios were observed in more mature vs. naïve Tαβ-cell subsets (vs. total T-cells). In 112/117 (96%) samples containing clonal Tαβ-cells in which the approach was validated, monotypic expression of TRBC1 was confirmed. Dilutional experiments showed a level of detection for detecting clonal Tαβ-cells of ≤10−4 in seven out of eight pathological samples. These results support implementation of the optimized TRBC1-FCM approach as a fast, specific and accurate method for assessing T-cell clonality in diagnostic-FCM panels, and for minimal (residual) disease detection in mature Tαβ+ leukemia/lymphoma patients.

Advances in the assessment of T-cell clonality

2020 ◽  
Vol 26 (9) ◽  
pp. 388-397
Author(s):  
Kate Davies ◽  
Joy Staniforth ◽  
William Haowei Xie ◽  
Hongxiang Liu ◽  
Maryam Salimi ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Clonality ◽  
Cell Clonality
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