scholarly journals Image‐Based Cell Profiling Enables Quantitative Tissue Microscopy in Gastroenterology

2020 ◽  
Vol 97 (12) ◽  
pp. 1222-1237 ◽  
Author(s):  
John W. Wills ◽  
Jack Robertson ◽  
Huw D. Summers ◽  
Michelle Miniter ◽  
Claire Barnes ◽  
...  
Keyword(s):  
Tissue Microscopy

Bilateral periorbital necrotising fasciitis associated with invasive group: a Streptococcus infection

2020 ◽  
Vol 13 (12) ◽  
pp. e236800
Author(s):  
Grace Anne McCabe ◽  
Thomas Hardy ◽  
Thomas Gordon Campbell
Keyword(s):  
Group A Streptococcus ◽  
Early Recognition ◽  
Skin Grafting ◽  
Final Diagnosis ◽  
Necrotising Fasciitis ◽  
Invasive Group ◽  
Group A ◽  
Tissue Microscopy ◽  
Periorbital Swelling ◽  
Multidisciplinary Teamwork

A previously independent 56-year-old immunocompetent woman presented with septic shock in the setting of periorbital swelling and diffuse infiltrates on chest imaging. Blood cultures were positive for growth of group A Streptococcus (GAS). Broad spectrum antimicrobials were initiated with the inclusion of the antitoxin agent clindamycin. Necrosis of periorbital tissue was noted and surgical consultation was obtained. Débridement of both eyelids with skin grafting was performed. GAS was isolated from wound cultures and also observed on periorbital tissue microscopy. The final diagnosis was bilateral periorbital necrotising fasciitis (PONF) associated with invasive GAS infection. The patient had a prolonged intensive care unit course with input from multiple specialist teams. This case demonstrates the importance of early recognition and treatment of PONF, the profound systemic morbidity caused by these infections, and illustrates successful multidisciplinary teamwork.

Circlet transform in cell and tissue microscopy

2020 ◽  
Vol 124 ◽  
pp. 106000 ◽  
Author(s):  
O. Sarrafzadeh ◽  
H. Rabbani ◽  
A. Mehri Dehnavi ◽  
A. Talebi
Keyword(s):  
Tissue Microscopy

scholarly journals Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy

2020 ◽  
Vol 6 (22) ◽  
pp. eaba4542 ◽  
Author(s):  
Chenyi Mao ◽  
Min Yen Lee ◽  
Jing-Ru Jhan ◽  
Aaron R. Halpern ◽  
Marcus A. Woodworth ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescent Dyes ◽  
Super Resolution ◽  
Fluorescent Labeling ◽  
Processing Conditions ◽  
Sample Processing ◽  
Tissue Fluorescence ◽  
Uniform Labeling ◽  
Tissue Microscopy

Fluorescence microscopy is a workhorse tool in biomedical imaging but often poses substantial challenges to practitioners in achieving bright or uniform labeling. In addition, while antibodies are effective specific labels, their reproducibility is often inconsistent, and they are difficult to use when staining thick specimens. We report the use of conventional, commercially available fluorescent dyes for rapid and intense covalent labeling of proteins and carbohydrates in super-resolution (expansion) microscopy and cleared tissue microscopy. This approach, which we refer to as Fluorescent Labeling of Abundant Reactive Entities (FLARE), produces simple and robust stains that are modern equivalents of classic small-molecule histology stains. It efficiently reveals a wealth of key landmarks in cells and tissues under different fixation or sample processing conditions and is compatible with immunolabeling of proteins and in situ hybridization labeling of nucleic acids.

scholarly journals Simple Chemical Stains for Feature-Rich Super-Resolution and Cleared-Tissue Microscopy

2019 ◽  
Vol 25 (S2) ◽  
pp. 1202-1203
Author(s):  
Chenyi Mao ◽  
Min Yen Lee ◽  
Jing-Ru Jhan ◽  
Joshua C. Vaughan
Keyword(s):  
Super Resolution ◽  
Simple Chemical ◽  
Tissue Microscopy

Autofocusing for tissue microscopy

1993 ◽  
Vol 11 (10) ◽  
pp. 629-639 ◽  
Author(s):  
TTE Yeo ◽  
SH Ong ◽  
Jayasooriah ◽  
R Sinniah
Keyword(s):  
Tissue Microscopy

scholarly journals Multiscale 3-dimensional pathology findings of COVID-19 diseased lung using high-resolution cleared tissue microscopy

2020 ◽  
Author(s):  
Guang Li ◽  
Sharon E. Fox ◽  
Brian Summa ◽  
Bihe Hu ◽  
Carola Wenk ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Labeling ◽  
Direct Result ◽  
Specimen Preparation ◽  
Tissue Microstructure ◽  
3 Dimensional ◽  
Frontline Treatment ◽  
Autopsy Tissues ◽  
Tissue Microscopy ◽  
Diseased Lung

AbstractThe study of pulmonary samples from individuals who have died as a direct result of COVID-19 infection is vital to our understanding of the pathogenesis of this disease. Histopathologic studies of lung tissue from autopsy of patients with COVID-19 specific mortality are only just emerging. All existing reports have relied on traditional 2-dimensional slide-based histological methods for specimen preparation. However, emerging methods for high-resolution, massively multiscale imaging of tissue microstructure using fluorescence labeling and tissue clearing methods enable the acquisition of tissue histology in 3-dimensions, that could open new insights into the nature of SARS-Cov-2 infection and COVID-19 disease processes. In this article, we present the first 3-dimensional images of lung autopsy tissues taken from a COVID-19 patient, including 3D “virtual histology” of cubic-millimeter volumes of the diseased lung, providing unique insights into disease processes contributing to mortality that could inform frontline treatment decisions.

Case 47

2020 ◽  
pp. 319-324
Author(s):  
Hilary Humphreys
Keyword(s):  
Solid Organ Transplantation ◽  
High Risk Patient ◽  
Risk Patient ◽  
Solid Organ ◽  
Persistent Symptoms ◽  
Patient Groups ◽  
Pcr Assays ◽  
Aggressive Debridement ◽  
Tissue Microscopy ◽  
Acute Myeloid

Mucormycosis can mimic aspergillosis, both clinically and radiologically. High-risk patient groups include those with acute myeloid leukaemia, following stem cell transplantation, solid organ transplantation, and poorly controlled diabetes mellitus, such as diabetic ketoacidosis against a background of poor social conditions. Persistent symptoms of sinusitis in an at-risk patient, should suggest rhinocerebral mucormycosis. Direct tissue microscopy with specialized stains (e.g. Grocot) is the mainstay of diagnosis but pan-fungal PCR assays are being evaluated. Aggressive debridement, which sometimes needs to be repeated, together with antifungal agents such as amphotericin B and/or, increasingly, posaconazole often for months, are required in the management of this challenging infection. However, the outcome is often dependant on the underlying condition, degree of immunosuppression, and whether it can be reduced.

Innovations in two-photon deep tissue microscopy

1999 ◽  
Vol 18 (5) ◽  
pp. 23-30 ◽  
Author(s):  
C. Buehler ◽  
Ki Hean Kim ◽  
C.Y. Dong ◽  
B.R. Masters ◽  
P.T.C. So
Keyword(s):  
Deep Tissue ◽  
Two Photon ◽  
Tissue Microscopy
Sign in / Sign up

Export Citation Format

Share Document