scholarly journals Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on “click chemistry”

2011 ◽  
Vol 79A (5) ◽  
pp. 328-337 ◽  
Author(s):  
Zbigniew Darzynkiewicz ◽  
Frank Traganos ◽  
Hong Zhao ◽  
H. Dorota Halicka ◽  
Jiangwei Li
Keyword(s):  
Dna Replication ◽  
Click Chemistry ◽  
Historical Perspective ◽  
Rna Synthesis ◽  
Recent Advances

Nuclear Distribution of Prothymosin α and Parathymosin: Evidence That Prothymosin α Is Associated with RNA Synthesis Processing and Parathymosin with Early DNA Replication

2000 ◽  
Vol 257 (1) ◽  
pp. 152-161 ◽  
Author(s):  
Katerina Vareli ◽  
Maria Frangou-Lazaridis ◽  
Ineke van der Kraan ◽  
Orestes Tsolas ◽  
Roel van Driel
Keyword(s):  
Dna Replication ◽  
Rna Synthesis ◽  
Prothymosin Α ◽  
Nuclear Distribution

scholarly journals Molecular biology of potexviruses: recent advances

2007 ◽  
Vol 88 (6) ◽  
pp. 1643-1655 ◽  
Author(s):  
Jeanmarie Verchot-Lubicz ◽  
Chang-Ming Ye ◽  
Devinka Bamunusinghe
Keyword(s):  
Gene Silencing ◽  
Rna Synthesis ◽  
Triple Gene Block ◽  
Potato Virus X ◽  
Future Research ◽  
Virus Movement ◽  
Suppressor Activity ◽  
Gene Block ◽  
Recent Advances

Recent advances in potexvirus research have produced new models describing virus replication, cell-to-cell movement, encapsidation, R gene-mediated resistance and gene silencing. Interactions between distant RNA elements are a central theme in potexvirus replication. The 5′ non-translated region (NTR) regulates genomic and subgenomic RNA synthesis and encapsidation, as well as virus plasmodesmal transport. The 3′ NTR regulates both plus- and minus-strand RNA synthesis. How the triple gene-block proteins interact for virus movement is still elusive. As the potato virus X (PVX) TGBp1 protein gates plasmodesmata, regulates virus translation and is a suppressor of RNA silencing, further research is needed to determine how these properties contribute to propelling virus through the plasmodesmata. Specifically, TGBp1 suppressor activity is required for virus movement, but how the silencing machinery relates to plasmodesmata is not known. The TGBp2 and TGBp3 proteins are endoplasmic reticulum (ER)-associated proteins required for virus movement. TGBp2 associates with ER-derived vesicles that traffic along the actin network. Future research will determine whether the virus-induced vesicles are cytopathic structures regulating events along the ER or are vehicles carrying virus to the plasmodesmata for transfer into neighbouring cells. Efforts to assemble virions in vitro identified a single-tailed particle (STP) comprising RNA, coat protein (CP) and TGBp1. It has been proposed that TGBp1 aids in transport of virions or STP between cells and ensures translation of RNA in the receiving cells. PVX is also a tool for studying Avr–R gene interactions and gene silencing in plants. The PVX CP is the elicitor for the Rx gene. Recent reports of the PVX CP reveal how CP interacts with the Rx gene product.

Wachstumsparameter in normalen und durch Actinomycin verlängerten Zellzyklen von Tetrahymena / Growth Parameters in Normal and Actinomycin-induced prolonged Cell Cycles of Tetrahymena

1969 ◽  
Vol 24 (12) ◽  
pp. 1624-1629 ◽  
Author(s):  
Günter Cleffmann
Keyword(s):  
Cell Cycle ◽  
Protein Synthesis ◽  
Cell Division ◽  
Dna Replication ◽  
Cell Growth ◽  
Rna Synthesis ◽  
Growth Parameters ◽  
Average Duration ◽  
Cell Cycles ◽  
Growing Cell

Actinomycin in low concentration (0,2 μg/ml — 0,5 μg/ml) prolongs the average duration of the cell cycle of Tetrahymena considerably, but does not inhibit cell division completely. Some parameters of the growing cell have been tested in cell cycles extended in this way and compared to those of normally growing cells. The RNA synthesis of treated cells is reduced to such an extent that the RNA content per cell decreases during the prolonged cell cycle. Nevertheless cell growth, protein synthesis and DNA replication proceed at almost the same rate as in untreated cells. These findings indicate that the presence of actinomycin does not interfere with RNA fractions necessary for growth but reduce the synthesis of RNA fractions which are essential for cell division. Therefore a longer period is needed for their accumulation.

scholarly journals Boronic acid based dynamic click chemistry: recent advances and emergent applications

2021 ◽  
Author(s):  
Saurav Chatterjee ◽  
Eric V. Anslyn ◽  
Anupam Bandyopadhyay
Keyword(s):  
Click Chemistry ◽  
Boronic Acid ◽  
Recent Advances

Fundamental progress, current developments, and rapidly growing applications of iminoboronate and salicylhydroxamic–boronate conjugate esters are deliberated.

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