scholarly journals Proteome heterogeneity and malignancy detection in pancreatic cyst fluids

2021 ◽  
Vol 11 (8) ◽  
Author(s):  
Sheng Pan ◽  
Randall E. Brand ◽  
Lisa A. Lai ◽  
David W. Dawson ◽  
Timothy R. Donahue ◽  
...  
Keyword(s):  
Pancreatic Cyst ◽  
Cyst Fluids

scholarly journals Mass Spectrometry-Based Comprehensive Analysis of Pancreatic Cyst Fluids

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Agnieszka Paziewska ◽  
Marcin Polkowski ◽  
Tymon Rubel ◽  
Jakub Karczmarski ◽  
Anna Wiechowska-Kozlowska ◽  
...  
Keyword(s):  
Pancreatic Cyst ◽  
Proteolytic Enzymes ◽  
Needle Aspiration ◽  
Protein Profiling ◽  
Single Type ◽  
Blood Proteins ◽  
Cystic Neoplasms ◽  
Practical Applications ◽  
New Biomarkers ◽  
Cyst Fluids

Pancreatic cyst fluids (PCFs) enriched in tumour-derived proteins are considered a potential source of new biomarkers. This study aimed to determine compositional and quantitative differences between the degradome and proteome of PCFs aspirated from different types of pancreatic cyst lesions (PCLs). 91 patients who underwent endoscopic ultrasound-fine needle aspiration under routine clinical diagnosis of PCLs were enrolled. Four cysts were malignant (CAs), and 87 were nonmalignant and consisted of 18 intraductal papillary mucinous neoplasms (IPMNs), 14 mucinous cystic neoplasms (MCNs), nine serous cystic neoplasms (SCNs), 29 pseudocysts (PCs), and 17 unclassified. Profiles of the <5 kDa fraction, the degradome, and the trypsin-digested proteome were analysed using an LTQ-Orbitrap Elite mass spectrometer coupled with a nanoACQUITY LC system. Qualitative analyses identified 796 and 366 proteins in degradome and proteome, respectively, and 689 (77%) and 285 (78%) of them were present in the Plasma Proteome Database. Gene Ontology analysis showed a significant overrepresentation of peptidases and peptidases inhibitors in both datasets. In the degradome fraction, quantitative values were obtained for 6996 peptides originating from 657 proteins. Of these, 2287 peptides were unique to a single type, and 515 peptides, derived from 126 proteins, were shared across cyst types. 32 peptides originating from 12 proteins had differential (adjustedp-value ≤0.05, FC ≥1.5) abundance in at least one of the five cysts types. In proteome, relative expression was measured for 330 proteins. Of them, 33 proteins had significantly (adjustedp-value ≤0.05, FC ≥1.5) altered abundance in at least one of the studied groups and 19 proteins appeared to be unique to a given cyst type. PCFs are dominated by blood proteins and proteolytic enzymes. Although differences in PCF peptide composition and abundance could aid classification of PCLs, the unpredictable inherent PCF proteolytic activity may limit the practical applications of PCF protein profiling.

scholarly journals Somatic molecular analysis augments cytologic evaluation of pancreatic cyst fluids as a diagnostic tool

Oncotarget ◽  
2019 ◽  
Vol 10 (40) ◽  
pp. 4026-4037
Author(s):  
Ali Sakhdari ◽  
Parnian Ahmadi Moghaddam ◽  
Chi Young Ok ◽  
Otto Walter ◽  
Keith Tomaszewicz ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Diagnostic Tool ◽  
Pancreatic Cyst ◽  
Cyst Fluids

Mutational Mosaics of Cell-Free DNA from Pancreatic Cyst Fluids

2020 ◽  
Vol 65 (8) ◽  
pp. 2294-2301 ◽  
Author(s):  
Agnieszka Paziewska ◽  
Marcin Polkowski ◽  
Krzysztof Goryca ◽  
Jakub Karczmarski ◽  
Anna Wiechowska-Kozlowska ◽  
...  
Keyword(s):  
Pancreatic Cyst ◽  
Cell Free Dna ◽  
Free Dna ◽  
Cyst Fluids

Proteases Present in Some Pancreatic Cyst Fluids May Affect Mucin Immunoassay by Degrading Antibodies and Antigens

Pancreas ◽  
2010 ◽  
Vol 39 (7) ◽  
pp. 1070-1076 ◽  
Author(s):  
Marie-Elisabeth Forgue-Lafitte ◽  
Ritu Arambam ◽  
Jacques Bara
Keyword(s):  
Pancreatic Cyst ◽  
Cyst Fluids

scholarly journals Glycomic and Proteomic Profiling of Pancreatic Cyst Fluids Identifies Hyperfucosylated Lactosamines on theN-linked Glycans of Overexpressed Glycoproteins

2012 ◽  
Vol 11 (7) ◽  
pp. M111.015792 ◽  
Author(s):  
Benjamin F. Mann ◽  
John A. Goetz ◽  
Michael G. House ◽  
C. Max Schmidt ◽  
Milos V. Novotny
Keyword(s):  
Pancreatic Cyst ◽  
Proteomic Profiling ◽  
Cyst Fluids

scholarly journals Proteomic Analyses of Pancreatic Cyst Fluids

Pancreas ◽  
2009 ◽  
Vol 38 (2) ◽  
pp. e33-e42 ◽  
Author(s):  
Eileen Ke ◽  
Bhavinkumar B. Patel ◽  
Tiffany Liu ◽  
Xin-Ming Li ◽  
Oleh Haluszka ◽  
...  
Keyword(s):  
Pancreatic Cyst ◽  
Proteomic Analyses ◽  
Cyst Fluids

Measurement of pS2 protein in pancreatic cyst fluids

1998 ◽  
Vol 24 (3) ◽  
pp. 181-186
Author(s):  
Jane M. Yang ◽  
Jeannie Lee ◽  
James F. Southern ◽  
Andrew L. Warshaw ◽  
Ela Dhanak ◽  
...  
Keyword(s):  
Pancreatic Cyst ◽  
Cyst Fluids ◽  
Ps2 Protein

Mo1212 Correlation of GNAS Mutation With Diagnostic Information in Pancreatic Cyst Fluids

2012 ◽  
Vol 142 (5) ◽  
pp. S-623
Author(s):  
Eric Ellsworth ◽  
Jamie Bleicher ◽  
Brittany M. Bullion ◽  
Nicole L. Klein ◽  
W. Christine Spence ◽  
...  
Keyword(s):  
Pancreatic Cyst ◽  
Diagnostic Information ◽  
Gnas Mutation ◽  
Cyst Fluids

Outcomes after Mutational Profiling of Pancreatic Cyst Fluids

2012 ◽  
Vol 107 ◽  
pp. S597
Author(s):  
Damien Mallat ◽  
Eric Ellsworth ◽  
Brendan Corcoran ◽  
Ann-Marie Terry ◽  
Syd Finkelstein
Keyword(s):  
Pancreatic Cyst ◽  
Mutational Profiling ◽  
Cyst Fluids
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