scholarly journals Somatic molecular analysis augments cytologic evaluation of pancreatic cyst fluids as a diagnostic tool

Oncotarget ◽  
2019 ◽  
Vol 10 (40) ◽  
pp. 4026-4037
Author(s):  
Ali Sakhdari ◽  
Parnian Ahmadi Moghaddam ◽  
Chi Young Ok ◽  
Otto Walter ◽  
Keith Tomaszewicz ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Diagnostic Tool ◽  
Pancreatic Cyst ◽  
Cyst Fluids

scholarly journals Mass Spectrometry-Based Comprehensive Analysis of Pancreatic Cyst Fluids

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Agnieszka Paziewska ◽  
Marcin Polkowski ◽  
Tymon Rubel ◽  
Jakub Karczmarski ◽  
Anna Wiechowska-Kozlowska ◽  
...  
Keyword(s):  
Pancreatic Cyst ◽  
Proteolytic Enzymes ◽  
Needle Aspiration ◽  
Protein Profiling ◽  
Single Type ◽  
Blood Proteins ◽  
Cystic Neoplasms ◽  
Practical Applications ◽  
New Biomarkers ◽  
Cyst Fluids

Pancreatic cyst fluids (PCFs) enriched in tumour-derived proteins are considered a potential source of new biomarkers. This study aimed to determine compositional and quantitative differences between the degradome and proteome of PCFs aspirated from different types of pancreatic cyst lesions (PCLs). 91 patients who underwent endoscopic ultrasound-fine needle aspiration under routine clinical diagnosis of PCLs were enrolled. Four cysts were malignant (CAs), and 87 were nonmalignant and consisted of 18 intraductal papillary mucinous neoplasms (IPMNs), 14 mucinous cystic neoplasms (MCNs), nine serous cystic neoplasms (SCNs), 29 pseudocysts (PCs), and 17 unclassified. Profiles of the <5 kDa fraction, the degradome, and the trypsin-digested proteome were analysed using an LTQ-Orbitrap Elite mass spectrometer coupled with a nanoACQUITY LC system. Qualitative analyses identified 796 and 366 proteins in degradome and proteome, respectively, and 689 (77%) and 285 (78%) of them were present in the Plasma Proteome Database. Gene Ontology analysis showed a significant overrepresentation of peptidases and peptidases inhibitors in both datasets. In the degradome fraction, quantitative values were obtained for 6996 peptides originating from 657 proteins. Of these, 2287 peptides were unique to a single type, and 515 peptides, derived from 126 proteins, were shared across cyst types. 32 peptides originating from 12 proteins had differential (adjustedp-value ≤0.05, FC ≥1.5) abundance in at least one of the five cysts types. In proteome, relative expression was measured for 330 proteins. Of them, 33 proteins had significantly (adjustedp-value ≤0.05, FC ≥1.5) altered abundance in at least one of the studied groups and 19 proteins appeared to be unique to a given cyst type. PCFs are dominated by blood proteins and proteolytic enzymes. Although differences in PCF peptide composition and abundance could aid classification of PCLs, the unpredictable inherent PCF proteolytic activity may limit the practical applications of PCF protein profiling.

Mo1516 Does Molecular Analysis Alter Pancreatic Cyst Management?

2013 ◽  
Vol 77 (5) ◽  
pp. AB411
Author(s):  
David Arner ◽  
Henry C. Ho ◽  
Bradley J. Weinbaum ◽  
Uzma D. Siddiqui ◽  
Harry R. Aslanian ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Pancreatic Cyst

Microsatellite instability diagnosis using the fully automated Idylla platform: feasibility study of an in-house rapid molecular testing ancillary to immunohistochemistry in pathology laboratories

2019 ◽  
Vol 72 (12) ◽  
pp. 830-835 ◽  
Author(s):  
Laura Samaison ◽  
Mélanie Grall ◽  
Frédéric Staroz ◽  
Arnaud Uguen
Keyword(s):  
Microsatellite Instability ◽  
Feasibility Study ◽  
Molecular Analysis ◽  
Diagnostic Tool ◽  
Microsatellite Analysis ◽  
Molecular Testing ◽  
Molecular Analyses ◽  
Microsatellite Stability ◽  
Rapid Treatment

AimTo study the performances of the Idylla MSI Assay in the diagnosis of microsatellite instability (MSI) or microsatellite stability (MSS).MethodsWe selected 12 tumour samples previously tested for MSI focusing on cases with discrepant results between MLH1, PMS2, MSH2 and MSH6 immunohistochemistry and microsatellite molecular analyses (five cases) or doubtful immunohistochemistry (two cases). Idylla MSI Assay was compared with retrospective immunohistochemistry and molecular results.ResultsIdylla MSI Assay showed an almost perfect concordance with microsatellite analysis results previously obtained (only one case with not fully conclusive analysis due to sample exhaustion). The full molecular analysis took less than 150 min per sample and revealed no mutation in any of the seven microsatellite sequences in five MSS samples and four to six mutated ones in seven MSI-High samples.ConclusionAt the era when the determination of MSI/MSS status is becoming important for rapid treatment choices, the Idylla MSI Assay consists of a valuable easy-to-perform diagnostic tool that allows, complementary to MLH1, PMS2, MSH2 and MSH6 immunohistochemistry, the diagnosis of MSI/MSS status in a single day.

Mutational Mosaics of Cell-Free DNA from Pancreatic Cyst Fluids

2020 ◽  
Vol 65 (8) ◽  
pp. 2294-2301 ◽  
Author(s):  
Agnieszka Paziewska ◽  
Marcin Polkowski ◽  
Krzysztof Goryca ◽  
Jakub Karczmarski ◽  
Anna Wiechowska-Kozlowska ◽  
...  
Keyword(s):  
Pancreatic Cyst ◽  
Cell Free Dna ◽  
Free Dna ◽  
Cyst Fluids
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