scholarly journals Development of KASP markers for wheat greenbug resistance gene Gb5

Crop Science ◽  
2020 ◽  
Author(s):  
Xiangyang Xu ◽  
Genqiao Li ◽  
Guihua Bai ◽  
Amy Bernardo ◽  
Brett F. Carver ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Greenbug Resistance ◽  
Kasp Markers

scholarly journals Characterization of RsgWBDC053: A New Greenbug Resistance Gene From Wild Barley (Hordeum Vulgare ssp. Spontaneum)

2021 ◽  
Author(s):  
Xiangyang Xu ◽  
Dolores Mornhinweg ◽  
Amy Bernardo ◽  
Genqiao Li ◽  
Ruolin Bian ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Wild Barley ◽  
Insect Pest ◽  
Genotyping By Sequencing ◽  
Nucleotide Polymorphisms ◽  
Barley Accession ◽  
Improvement Programs ◽  
Greenbug Resistance ◽  
Kasp Markers

Abstract Greenbug (Schizaphis graminum Rondani) is a destructive insect pest that not only damages plants, but also serves as a vector for many viruses. Host plant resistance is the preferred strategy for managing greenbug. To date, only two greenbug resistance genes, Rsg1 and Rsg2, have been reported in barley, with only the former being deployed in cultivars. To breed cultivars with effective resistance against various greenbug biotypes, additional resistance genes are urgently needed to sustain barley production. Wild barley accession WBDC053 (PI 681777), originating from the Baluchistan region of Pakistan, was previously found to be resistant to several greenbug biotypes. In this study, a recombinant inbred line (RIL) population derived from Weskan × WBDC053 was evaluated for response to greenbug biotype E and genotyped using genotyping by sequencing (GBS). A set of 3,347 high quality GBS-derived single nucleotide polymorphisms (SNPs) were then used to map a greenbug resistance gene in this wild barley accession. Linkage analysis placed the greenbug resistance gene in WBDC053, temporarily designated RsgWBDC053, in a 2.35 Mb interval (0-2,354,645 bp) in the terminal region of the short arm of chromosome 2H. This interval harbors 15 genes with leucine-rich-repeat (LRR) protein domains. An allelism test between WBDC053 carrying RsgWBDC053 and STARS1501B carrying Rsg2 indicated that the former is either allelic or closely linked to the latter. GBS-SNPs 2H_1318811 and 2H_1839499 co-segregated with RsgWBDC053 and were converted to Kompetitive allele specific PCR (KASP) markers, KASP-Rsg053-1 and KASP-Rsg0533-2. The two KASP markers can be used to select for RsgWBDC053, but also have the potential to tag Rsg2 in barley improvement programs.

Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat

2017 ◽  
Vol 130 (9) ◽  
pp. 1867-1884 ◽  
Author(s):  
Chor-Tee Tan ◽  
Hangjin Yu ◽  
Yan Yang ◽  
Xiangyang Xu ◽  
Mingshun Chen ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Hessian Fly ◽  
Hessian Fly Resistance ◽  
Development And Validation ◽  
Greenbug Resistance ◽  
Kasp Markers

Characterization of an incomplete leaf rust resistance gene on chromosome 1RS and development of KASP markers for Lr47 in wheat

2020 ◽  
Author(s):  
Xiangyang Xu ◽  
Genqiao Li ◽  
Guihua Bai ◽  
Amy Bernardo ◽  
Brett F Carver ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Great Plains ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Breeding Lines ◽  
Chromosome Arm ◽  
Kasp Markers

Leaf rust, caused by Puccininia triticina (Pt), is one of the most common wheat diseases in the Great Plains of the USA. A population of recombinant inbred lines (RILs) from CI 17884 x Bainong 418 was evaluated for responses to leaf rust race Pt52-2 and genotyped using single nucleotide polymorphism (SNP) markers. Quantitative trait locus (QTL) analysis identified a minor gene for resistance to leaf rust, designated QLr.stars-1RS, on the 1BL.1RS translocation segment in Bainong 418, and another leaf rust resistance gene, Lr47, on chromosome 7A of CI 17884. Lr47, originally identified in CI 17884 and located in a wheat-T. speltoides translocation segment 7S#1S, remains one of only a few race-specific resistance genes still effective in the Great Plains. A set of 7A-specific simple sequence repeat (SSR) markers were developed and used to genotype CI 17884 and a pair of near-isogenic lines differing in the presence or absence of 7S#1S, PI 603918 and Pavon F76. Haplotype analysis indicated that the estimated length of 7S#1S was 157.23 to 174.42 Mb, accounting for about 23% of the 7A chromosome. Two SNPs on 7S#1S and 4 SNPs on the 1RS chromosome arm were converted to KASP markers, which were subsequently validated in a panel of cultivars and recently released elite breeding lines. Of these, one and two KASP markers are specific to the 1RS chromosome arm and 7S#1S, respectively, indicating that they can facilitate the introgression of Lr47 and QLr.stars-1BS into locally adapted wheat cultivars and breeding lines.

Linkage mapping of powdery mildew and greenbug resistance genes on recombinant 1RS from 'Amigo' and 'Kavkaz' wheat–rye translocations of chromosome 1RS.1AL

Genome ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 292-298 ◽  
Author(s):  
Yehia Mater ◽  
Stephen Baenziger ◽  
Kulvinder Gill ◽  
Robert Graybosch ◽  
Lynda Whitcher ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Dna Sequences ◽  
Secale Cereale ◽  
Polyacrylamide Gel Electrophoresis ◽  
Rflp Markers ◽  
Resistance Locus ◽  
Greenbug Resistance

Cultivated rye (Secale cereale L., 2n = 2x = 14, RR) is an important source of genes for insect and disease resistance in wheat (Triticum aestivum L., 2n = 6x = 42). Rye chromosome arm 1RS of S. cereale 'Kavkaz' originally found as a 1BL.1RS translocation, carries genes for disease resistance (e.g., Lr26, Sr31, Yr9, and Pm8), while 1RS of the S. cereale 'Amigo' translocation (1RSA) carries a single resistance gene for greenbug (Schizaphis graminum Rondani) biotypes B and C and also carries additional disease-resistance genes. The purpose of this research was to identify individual plants that were recombinant in the homologous region of.1AL.1RSV and 1AL.1RSA using both molecular and phenotypic markers. Secale cereale 'Nekota' (1AL.1RSA) and S. cereale 'Pavon 76' (1AL.1RSV) were mated and the F1 was backcrossed to 'Nekota' (1AL.1AS) to generate eighty BC1F2:3 families (i.e., ('Nekota' 1AL.1RSA × 'Pavon 76' 1AL.1RSV) × 'Nekota' 1AL.1AS). These families were genotyped using the secalin–gliadin grain storage protein banding pattern generated with polyacrylamide gel electrophoresis to discriminate 1AL.1AS/1AL.1RS heterozygotes from the 1AL.1RSA+V and 1AL.1AS homozygotes. Segregation of the secalin locus and PCR markers based on the R173 family of rye specific repeated DNA sequences demonstrated the presence of recombinant 1AL.1RSA+V families. Powdery mildew (Blumeria graminis) and greenbug resistance genes on the recombinant 1RSA+V arm were mapped in relation to the Sec-1 locus, 2 additional protein bands, 3 SSRs, and 13 RFLP markers. The resultant linkage map of 1RS spanned 82.4 cM with marker order and spacing showing reasonable agreement with previous maps of 1RS. Fifteen markers lie within a region of 29.7 cM next to the centromere, yet corresponded to just 36% of the overall map length. The map position of the RFLP marker probe mwg68 was 10.9 cM distal to the Sec-1 locus and 7.8 cM proximal to the powdery mildew resistance locus. The greenbug resistance gene was located 2.7 cM proximal to the Sec-1 locus.Key words: microsatellites, SSRs, RFLP, secalin-gliadin, alien genes introgression.

Development and Validation of KASP Markers for Stv-bi, a Rice Stripe Virus Resistance Gene in Rice (Oryza sativa L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 196-201 ◽  
Author(s):  
Ju-Won Kang ◽  
Sais-Beul Lee ◽  
Ji-Yun Lee ◽  
Young-Ho Kwon ◽  
So-Myeong Lee ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Oryza Sativa L ◽  
Rice Stripe Virus ◽  
Development And Validation ◽  
Kasp Markers ◽  
Virus Resistance Gene

scholarly journals BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Hana Šimková ◽  
Jan Šafář ◽  
Marie Kubaláková ◽  
Pavla Suchánková ◽  
Jarmila Číhalíková ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Positional Cloning ◽  
Cost Effective ◽  
Wheat Chromosome ◽  
Aphid Resistance ◽  
Insert Size ◽  
High Coverage ◽  
Bac Libraries ◽  
Greenbug Resistance ◽  
Targeted Approach

Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than million clones, which makes their screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance geneDnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistance geneGb3. The small number of clones combined with high coverage made the screening highly efficient and cost effective.

scholarly journals Development and Validation of KASP Markers for Wheat Streak Mosaic Virus Resistance Gene Wsm2

Crop Science ◽  
2016 ◽  
Vol 57 (1) ◽  
pp. 340-349 ◽  
Author(s):  
Chor-Tee Tan ◽  
Silvano Assanga ◽  
Guorong Zhang ◽  
Jackie C. Rudd ◽  
Scott D. Haley ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Wheat Streak Mosaic Virus ◽  
Development And Validation ◽  
Kasp Markers ◽  
Virus Resistance Gene
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