KASP Markers for Wheat Greenbug Resistance

CSA News ◽  
2020 ◽  
Keyword(s):  
Greenbug Resistance ◽  
Kasp Markers

scholarly journals Development of KASP markers for wheat greenbug resistance gene Gb5

Crop Science ◽  
2020 ◽  
Author(s):  
Xiangyang Xu ◽  
Genqiao Li ◽  
Guihua Bai ◽  
Amy Bernardo ◽  
Brett F. Carver ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Greenbug Resistance ◽  
Kasp Markers

scholarly journals Characterization of RsgWBDC053: A New Greenbug Resistance Gene From Wild Barley (Hordeum Vulgare ssp. Spontaneum)

2021 ◽  
Author(s):  
Xiangyang Xu ◽  
Dolores Mornhinweg ◽  
Amy Bernardo ◽  
Genqiao Li ◽  
Ruolin Bian ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Wild Barley ◽  
Insect Pest ◽  
Genotyping By Sequencing ◽  
Nucleotide Polymorphisms ◽  
Barley Accession ◽  
Improvement Programs ◽  
Greenbug Resistance ◽  
Kasp Markers

Abstract Greenbug (Schizaphis graminum Rondani) is a destructive insect pest that not only damages plants, but also serves as a vector for many viruses. Host plant resistance is the preferred strategy for managing greenbug. To date, only two greenbug resistance genes, Rsg1 and Rsg2, have been reported in barley, with only the former being deployed in cultivars. To breed cultivars with effective resistance against various greenbug biotypes, additional resistance genes are urgently needed to sustain barley production. Wild barley accession WBDC053 (PI 681777), originating from the Baluchistan region of Pakistan, was previously found to be resistant to several greenbug biotypes. In this study, a recombinant inbred line (RIL) population derived from Weskan × WBDC053 was evaluated for response to greenbug biotype E and genotyped using genotyping by sequencing (GBS). A set of 3,347 high quality GBS-derived single nucleotide polymorphisms (SNPs) were then used to map a greenbug resistance gene in this wild barley accession. Linkage analysis placed the greenbug resistance gene in WBDC053, temporarily designated RsgWBDC053, in a 2.35 Mb interval (0-2,354,645 bp) in the terminal region of the short arm of chromosome 2H. This interval harbors 15 genes with leucine-rich-repeat (LRR) protein domains. An allelism test between WBDC053 carrying RsgWBDC053 and STARS1501B carrying Rsg2 indicated that the former is either allelic or closely linked to the latter. GBS-SNPs 2H_1318811 and 2H_1839499 co-segregated with RsgWBDC053 and were converted to Kompetitive allele specific PCR (KASP) markers, KASP-Rsg053-1 and KASP-Rsg0533-2. The two KASP markers can be used to select for RsgWBDC053, but also have the potential to tag Rsg2 in barley improvement programs.

Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat

2017 ◽  
Vol 130 (9) ◽  
pp. 1867-1884 ◽  
Author(s):  
Chor-Tee Tan ◽  
Hangjin Yu ◽  
Yan Yang ◽  
Xiangyang Xu ◽  
Mingshun Chen ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Hessian Fly ◽  
Hessian Fly Resistance ◽  
Development And Validation ◽  
Greenbug Resistance ◽  
Kasp Markers

scholarly journals Identifying new variation at the J locus, previously identified as e6, in long juvenile ‘Paranagoiana’ soybean

2021 ◽  
Author(s):  
Nour Nissan ◽  
Elroy R. Cober ◽  
Michael Sadowski ◽  
Martin Charette ◽  
Ashkan Golshani ◽  
...  
Keyword(s):  
Single Gene ◽  
Pcr Amplification ◽  
Gene Control ◽  
Grain Yields ◽  
Short Day ◽  
Exon 4 ◽  
Short Days ◽  
Kasp Markers

Abstract Key message A previously identified soybean maturity locus, E6, is discovered to be J, with the long juvenile allele in Paranagoiana now deemed j−x. Abstract Soybean grown at latitudes of ~20° or lower can produce lower grain yields due to the short days. This limitation can be overcome by using the long juvenile trait (LJ) which delays flowering under short day conditions. Two LJ loci have been mapped to the same location on Gm04, J and E6. The objective of this research was to investigate the e6 allele in ‘Paranagoiana’ and determine if E6 and J are the same locus or linked loci. KASP markers showed that e6 lines did not have the j−1 allele of LJ PI 159925. A population fixed for E1 but segregating for E6, with e6 introgressed from Paranagoiana, showed single gene control for flowering and maturity under short days. Sequencing Glyma.04G050200, the J gene, with long amplification Taq found that the e6 line ‘Paranagoiana’ contains a Ty1-copia retrotransposon of ~10,000 bp, inserted within exon 4. PCR amplification of the cDNA of Glyma.04G050200 also showed differences between the mRNA sequences (presence of insertion in j−x). Hence, we conclude that the loci E6 and J are one locus and deem this new variation found in Paranagoiana as j−x.

Trisomic Analysis of Greenbug Resistance in Barley, Hordeum vulgare L. 1

Crop Science ◽  
1973 ◽  
Vol 13 (6) ◽  
pp. 684-685 ◽  
Author(s):  
J. H. Gardenhire ◽  
N. A. Tuleen ◽  
K. W. Stewart
Keyword(s):  
Hordeum Vulgare ◽  
Hordeum Vulgare L ◽  
Trisomic Analysis ◽  
Greenbug Resistance

Association of KASP markers with Hessian fly resistance in wheat of diverse origin

Euphytica ◽  
2018 ◽  
Vol 214 (8) ◽  
Author(s):  
Damian Collins ◽  
Livinus Emebiri ◽  
Mui-Keng Tan ◽  
Mustapha El Bouhssini ◽  
Ossie Wildman
Keyword(s):  
Hessian Fly ◽  
Hessian Fly Resistance ◽  
Diverse Origin ◽  
Kasp Markers

Inheritance of a Second Source of Greenbug Resistance in Barley 1

Crop Science ◽  
1987 ◽  
Vol 27 (2) ◽  
pp. 241-243 ◽  
Author(s):  
O. G. Merkle ◽  
J. A. Webster ◽  
G. H. Morgan
Keyword(s):  
Greenbug Resistance

Genetic mapping of QTLs associated with greenbug resistance and tolerance in Sorghum bicolor

2002 ◽  
Vol 104 (8) ◽  
pp. 1373-1378 ◽  
Author(s):  
H. Agrama ◽  
G. Widle ◽  
J. Reese ◽  
L. Campbell ◽  
M. Tuinstra
Keyword(s):  
Genetic Mapping ◽  
Sorghum Bicolor ◽  
Greenbug Resistance

scholarly journals Combining Single Nucleotide Polymorphism Genotyping Array with Bulked Segregant Analysis to Map a Gene Controlling Adult Plant Resistance to Stripe Rust in Wheat Line 03031-1-5 H62

2018 ◽  
Vol 108 (1) ◽  
pp. 103-113 ◽  
Author(s):  
Jianhui Wu ◽  
Qilin Wang ◽  
Liangsheng Xu ◽  
Xianming Chen ◽  
Bei Li ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Bulked Segregant Analysis ◽  
Snp Array ◽  
Adult Plant ◽  
Wheat Line ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Kasp Markers

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most devastating diseases of wheat worldwide. Growing resistant cultivars is considered the best approach to manage this disease. In order to identify the resistance gene(s) in wheat line 03031-1-5 H62, which displayed high resistance to stripe rust at adult plant stage, a cross was made between 03031-1-5 H62 and susceptible cultivar Avocet S. The mapping population was tested with Chinese P. striiformis f. sp. tritici race CYR32 through artificial inoculation in a field in Yangling, Shaanxi Province and under natural infection in Tianshui, Gansu Province. The segregation ratios indicated that the resistance was conferred by a single dominant gene, temporarily designated as YrH62. A combination of bulked segregant analysis (BSA) with wheat 90K single nucleotide polymorphism (SNP) array was used to identify molecular markers linked to YrH62. A total of 376 polymorphic SNP loci identified from the BSA analysis were located on chromosome 1B, from which 35 kompetitive allele-specific PCR (KASP) markers selected together with 84 simple sequence repeat (SSR) markers on 1B were used to screen polymorphism and a chromosome region associated with rust resistance was identified. To saturate the chromosomal region covering the YrH62 locus, a 660K SNP array was used to identify more SNP markers. To develop tightly linked markers for marker-assisted selection of YrH62 in wheat breeding, 18 SNPs were converted into KASP markers. A final linkage map consisting of 15 KASP and 3 SSR markers was constructed with KASP markers AX-109352427 and AX-109862469 flanking the YrH62 locus in a 1.0 cM interval. YrH62 explained 63.8 and 69.3% of the phenotypic variation for disease severity and infection type, respectively. YrH62 was located near the centromeric region of chromosome 1BS based on the positions of the SSR markers in 1B deletion bins. Based on the origin, responses to P. striiformis f. sp. tritici races, and marker distances, YrH62 is likely different from the other reported stripe rust resistance genes/quantitative trait loci on 1B. The gene and tightly linked KASP markers will be useful for breeding wheat cultivars with resistance to stripe rust.

scholarly journals Development of KASP Markers for Germplasm Characterization and Fingerprinting Identification of Broccoli in China

2020 ◽  
Author(s):  
Yusen Shen ◽  
Jiansheng Wang ◽  
Huifang Yu ◽  
Xiaoguang Sheng ◽  
Zhenqing Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Marker Assisted Selection ◽  
Genetic Relationships ◽  
Cost Effective ◽  
Germplasm Characterization ◽  
2D Barcode ◽  
New Type ◽  
Generation Sequencing ◽  
Kasp Markers ◽  
Selection Of

Abstract Background: Broccoli (Brassica oleracea var. italica) is a vegetable widely cultivated in China. Many new-type broccoli cultivars were bred and developed by Chinese breeders during the recent three decades. However, the broccoli cultivar nomenclature and detailed information of genetic relationships among broccoli germplasms are unclear. Results: The present study identified millions of SNPs by next-generation sequencing of 23 representative broccoli lines. Through several steps of selection, 100 SNPs were successfully converted into KASP markers, and used to evaluate the genetic diversity, genetic relationship, and population structure of 392 broccoli accessions, which represent the mainly broccoli breeding materials in China. The initial, introduced and improved accessions were well clustered, though some accessions were overlapped between groups, probably reflecting the fact that breeding activities led to genetic similarities. To make the KASP genotyping more efficient and cost-effective, 25 of the 100 KASPs were selected for fingerprinting of all accessions, and the 2D barcode contained fingerprinting information were generated for elite varieties. Conclusion: The KASP markers developed in this study provided an efficient way for germplasm characterization, DNA fingerprinting, seed purity identification, and marker-assisted selection of broccoli in China.

Sign in / Sign up

Export Citation Format

Share Document