Triple‐Decker Sandwich Cultures of Intestinal Organoids for Long‐Term Live Imaging, Uniform Perturbation, and Statistical Sampling

2022 ◽  
Vol 2 (1) ◽  
Author(s):  
Hailey M. Cambra ◽  
Naren P. Tallapragada ◽  
Prabhath Mannam ◽  
David T. Breault ◽  
Allon M. Klein
Keyword(s):  
Live Imaging ◽  
Statistical Sampling ◽  
Intestinal Organoids

Long Term Exposure to a Grape Seed Proanthocyanidin Extract Enhances L‐Cell Differentiation in Intestinal Organoids

2020 ◽  
Vol 64 (16) ◽  
pp. 2000303 ◽  
Author(s):  
Àngela Casanova‐Martí ◽  
Noemi González‐Abuín ◽  
Joan Serrano ◽  
Maria Teresa Blay ◽  
Ximena Terra ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Grape Seed ◽  
Grape Seed Proanthocyanidin Extract ◽  
Intestinal Organoids ◽  
L Cell ◽  
Grape Seed Proanthocyanidin

scholarly journals A coral-on-a-chip microfluidic platform enabling live-imaging microscopy of reef-building corals

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Orr H. Shapiro ◽  
Esti Kramarsky-Winter ◽  
Assaf R. Gavish ◽  
Roman Stocker ◽  
Assaf Vardi
Keyword(s):  
Live Imaging ◽  
Live Coral ◽  
Powerful Method ◽  
Microfluidic Platform ◽  
Experimental Platform ◽  
Micro Scale ◽  
Algal Symbionts ◽  
Spatio Temporal

Abstract Coral reefs, and the unique ecosystems they support, are facing severe threats by human activities and climate change. Our understanding of these threats is hampered by the lack of robust approaches for studying the micro-scale interactions between corals and their environment. Here we present an experimental platform, coral-on-a-chip, combining micropropagation and microfluidics to allow direct microscopic study of live coral polyps. The small and transparent coral micropropagates are ideally suited for live-imaging microscopy, while the microfluidic platform facilitates long-term visualization under controlled environmental conditions. We demonstrate the usefulness of this approach by imaging coral micropropagates at previously unattainable spatio-temporal resolutions, providing new insights into several micro-scale processes including coral calcification, coral–pathogen interaction and the loss of algal symbionts (coral bleaching). Coral-on-a-chip thus provides a powerful method for studying coral physiology in vivo at the micro-scale, opening new vistas in coral biology.

scholarly journals Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms

Autophagy ◽  
2015 ◽  
Vol 11 (9) ◽  
pp. 1561-1579 ◽  
Author(s):  
Monica Prado ◽  
Nina Eickel ◽  
Mariana De Niz ◽  
Anna Heitmann ◽  
Carolina Agop-Nersesian ◽  
...  
Keyword(s):  
Immune Responses ◽  
Live Imaging ◽  
Plasmodium Infection

scholarly journals Congruence of location-specific transcriptional programs in intestinal organoids during long-term culture

2019 ◽  
Author(s):  
B. van der Hee ◽  
O. Madsen ◽  
H. Smidt ◽  
J.M. Wells
Keyword(s):  
Gene Expression ◽  
Model System ◽  
Transformed Cells ◽  
Stable Model ◽  
Intestinal Development ◽  
Phenotypic Stability ◽  
Intestinal Organoids ◽  
Tissue Of Origin ◽  
Cancer Phenotypes

AbstractThe emergence of intestinal organoids, as a stem cell-based self-renewable model system, has led to many studies on intestinal development and cell-cell signaling. However, potential issues regarding the phenotypic stability and reproducibility of the methodology during culture still needs to be addressed for different organoids. Here we investigated the transcriptomes of intestinal organoids derived from the same pig as well as batch-to-batch variation of organoids derived from different pigs over long-term passage. The set of genes expressed in organoids closely resembled that of the tissue of origin, including location specific functions, for at least 17 passages. Minor differences in gene expression were observed between individual organoid cultures. In contrast, most tissue-specific genes were not expressed in the transformed jejunum cell line IPECJ2, which also showed gene expression consistent with cancer phenotypes. We conclude that intestinal organoids provide a robust and stable model for translational research with clear advantages over transformed cells.

scholarly journals In vivo live imaging of postnatal neural stem cells

Development ◽  
2021 ◽  
Vol 148 (18) ◽  
Author(s):  
Alina Marymonchyk ◽  
Sarah Malvaut ◽  
Armen Saghatelyan
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vivo Imaging ◽  
Live Imaging ◽  
Future Directions ◽  
Environmental Signals ◽  
New Information ◽  
Imaging Results

ABSTRACT Neural stem cells (NSCs) are maintained in specific regions of the postnatal brain and contribute to its structural and functional plasticity. However, the long-term renewal potential of NSCs and their mode of division remain elusive. The use of advanced in vivo live imaging approaches may expand our knowledge of NSC physiology and provide new information for cell replacement therapies. In this Review, we discuss the in vivo imaging methods used to study NSC dynamics and recent live-imaging results with respect to specific intracellular pathways that allow NSCs to integrate and decode different micro-environmental signals. Lastly, we discuss future directions that may provide answers to unresolved questions regarding NSC physiology.

Technique for Liquid Culture of Early Chick Embryos Suitable for Long Term Live Imaging

2015 ◽  
Author(s):  
Cornelis Weijer ◽  
Emil Rozbicki ◽  
Manli Chuai ◽  
Cornelis Jan Weijer
Keyword(s):  
Liquid Culture ◽  
Live Imaging ◽  
Chick Embryos

scholarly journals Long-term Live Imaging of Drosophila Eye Disc

10.3791/55748 ◽  
2017 ◽  
Author(s):  
Chia-Kang Tsao ◽  
Hui-Yu Ku ◽  
Y. Henry Sun
Keyword(s):  
Live Imaging ◽  
Eye Disc

scholarly journals Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163744 ◽  
Author(s):  
Chia-Kang Tsao ◽  
Hui-Yu Ku ◽  
Yuan-Ming Lee ◽  
Yu-Fen Huang ◽  
Yi Henry Sun
Keyword(s):  
Ex Vivo ◽  
Imaginal Discs ◽  
Live Imaging ◽  
Ex Vivo Culture
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