scholarly journals Long-term Live Imaging of Drosophila Eye Disc

10.3791/55748 ◽  
2017 ◽  
Author(s):  
Chia-Kang Tsao ◽  
Hui-Yu Ku ◽  
Y. Henry Sun
Keyword(s):  
Live Imaging ◽  
Eye Disc

scholarly journals A coral-on-a-chip microfluidic platform enabling live-imaging microscopy of reef-building corals

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Orr H. Shapiro ◽  
Esti Kramarsky-Winter ◽  
Assaf R. Gavish ◽  
Roman Stocker ◽  
Assaf Vardi
Keyword(s):  
Live Imaging ◽  
Live Coral ◽  
Powerful Method ◽  
Microfluidic Platform ◽  
Experimental Platform ◽  
Micro Scale ◽  
Algal Symbionts ◽  
Spatio Temporal

Abstract Coral reefs, and the unique ecosystems they support, are facing severe threats by human activities and climate change. Our understanding of these threats is hampered by the lack of robust approaches for studying the micro-scale interactions between corals and their environment. Here we present an experimental platform, coral-on-a-chip, combining micropropagation and microfluidics to allow direct microscopic study of live coral polyps. The small and transparent coral micropropagates are ideally suited for live-imaging microscopy, while the microfluidic platform facilitates long-term visualization under controlled environmental conditions. We demonstrate the usefulness of this approach by imaging coral micropropagates at previously unattainable spatio-temporal resolutions, providing new insights into several micro-scale processes including coral calcification, coral–pathogen interaction and the loss of algal symbionts (coral bleaching). Coral-on-a-chip thus provides a powerful method for studying coral physiology in vivo at the micro-scale, opening new vistas in coral biology.

scholarly journals Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms

Autophagy ◽  
2015 ◽  
Vol 11 (9) ◽  
pp. 1561-1579 ◽  
Author(s):  
Monica Prado ◽  
Nina Eickel ◽  
Mariana De Niz ◽  
Anna Heitmann ◽  
Carolina Agop-Nersesian ◽  
...  
Keyword(s):  
Immune Responses ◽  
Live Imaging ◽  
Plasmodium Infection

scholarly journals In vivo live imaging of postnatal neural stem cells

Development ◽  
2021 ◽  
Vol 148 (18) ◽  
Author(s):  
Alina Marymonchyk ◽  
Sarah Malvaut ◽  
Armen Saghatelyan
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vivo Imaging ◽  
Live Imaging ◽  
Future Directions ◽  
Environmental Signals ◽  
New Information ◽  
Imaging Results

ABSTRACT Neural stem cells (NSCs) are maintained in specific regions of the postnatal brain and contribute to its structural and functional plasticity. However, the long-term renewal potential of NSCs and their mode of division remain elusive. The use of advanced in vivo live imaging approaches may expand our knowledge of NSC physiology and provide new information for cell replacement therapies. In this Review, we discuss the in vivo imaging methods used to study NSC dynamics and recent live-imaging results with respect to specific intracellular pathways that allow NSCs to integrate and decode different micro-environmental signals. Lastly, we discuss future directions that may provide answers to unresolved questions regarding NSC physiology.

Technique for Liquid Culture of Early Chick Embryos Suitable for Long Term Live Imaging

2015 ◽  
Author(s):  
Cornelis Weijer ◽  
Emil Rozbicki ◽  
Manli Chuai ◽  
Cornelis Jan Weijer
Keyword(s):  
Liquid Culture ◽  
Live Imaging ◽  
Chick Embryos

scholarly journals Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163744 ◽  
Author(s):  
Chia-Kang Tsao ◽  
Hui-Yu Ku ◽  
Yuan-Ming Lee ◽  
Yu-Fen Huang ◽  
Yi Henry Sun
Keyword(s):  
Ex Vivo ◽  
Imaginal Discs ◽  
Live Imaging ◽  
Ex Vivo Culture

scholarly journals Abstract 2725: Long-term live imaging of ovarian cancer invasion using optical coherence tomography

2020 ◽  
Author(s):  
Amelia Hallas-Potts ◽  
Jonathan H. Mason ◽  
Jessica H. Lim ◽  
Michael Churchman ◽  
Pierre Bagnaninchi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Optical Coherence Tomography ◽  
Live Imaging ◽  
Cancer Invasion ◽  
Optical Coherence

Fabrication of microcage arrays to fix plant ovules for long-term live imaging and observation

2014 ◽  
Vol 191 ◽  
pp. 178-185 ◽  
Author(s):  
Jongho Park ◽  
Daisuke Kurihara ◽  
Tetsuya Higashiyama ◽  
Hideyuki Arata
Keyword(s):  
Live Imaging
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