scholarly journals Highly Selective, CRISPR/Cas9‐Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast

2021 ◽  
Vol 1 (8) ◽  
Author(s):  
Natalay Kouprina ◽  
Jung‐Hyun Kim ◽  
Vladimir Larionov
Keyword(s):  
Tar Cloning

scholarly journals The genomic structure of a human chromosome 22 nucleolar organizer region determined by TAR cloning

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jung-Hyun Kim ◽  
Vladimir N. Noskov ◽  
Aleksey Y. Ogurtsov ◽  
Ramaiah Nagaraja ◽  
Nikolai Petrov ◽  
...  
Keyword(s):  
Genomic Structure ◽  
Organizer Region ◽  
Nucleolar Organizer Region ◽  
Nucleolar Organizer ◽  
Chromosome 21 ◽  
Reference Sequence ◽  
Chromosome 22 ◽  
Rdna Variation ◽  
High Level ◽  
Tar Cloning

AbstractThe rDNA clusters and flanking sequences on human chromosomes 13, 14, 15, 21 and 22 represent large gaps in the current genomic assembly. The organization and the degree of divergence of the human rDNA units within an individual nucleolar organizer region (NOR) are only partially known. To address this lacuna, we previously applied transformation-associated recombination (TAR) cloning to isolate individual rDNA units from chromosome 21. That approach revealed an unexpectedly high level of heterogeneity in human rDNA, raising the possibility of corresponding variations in ribosome dynamics. We have now applied the same strategy to analyze an entire rDNA array end-to-end from a copy of chromosome 22. Sequencing of TAR isolates provided the entire NOR sequence, including proximal and distal junctions that may be involved in nucleolar function. Comparison of the newly sequenced rDNAs to reference sequence for chromosomes 22 and 21 revealed variants that are shared in human rDNA in individuals from different ethnic groups, many of them at high frequency. Analysis infers comparable intra- and inter-individual divergence of rDNA units on the same and different chromosomes, supporting the concerted evolution of rDNA units. The results provide a route to investigate further the role of rDNA variation in nucleolar formation and in the empirical associations of nucleoli with pathology.

Specific isolation of human rDNA genes by TAR cloning

Gene ◽  
1997 ◽  
Vol 197 (1-2) ◽  
pp. 269-276 ◽  
Author(s):  
Natalya Kouprina ◽  
Joan Graves ◽  
Michael R Cancilla ◽  
Michael A Resnick ◽  
Vladimir Larionov
Keyword(s):  
Rdna Genes ◽  
Tar Cloning

Transformation-Associated Recombination (TAR) Cloning of Tumor-Inducing Xmrk2 Gene from Xiphophorus maculatus

2001 ◽  
Vol 3 (0) ◽  
pp. S168-S176 ◽  
Author(s):  
Jakob M. Kirchner ◽  
Vesselina Ivanova ◽  
Vladimir N. Noskov ◽  
Autum Samson ◽  
Jean-Nicolas Volff ◽  
...  
Keyword(s):  
Xiphophorus Maculatus ◽  
Tar Cloning

Characterization of the hamster genomic fragment cloned by TAR cloning technology with interspecific sequence information

2012 ◽  
Vol 34 (6) ◽  
pp. 647-652
Author(s):  
Sang-Yeop Lee ◽  
Young-Ho Yoon ◽  
Si-Hoon Kim ◽  
Se-Ra Lee ◽  
Jeong-Min Chu ◽  
...  
Keyword(s):  
Sequence Information ◽  
Genomic Fragment ◽  
Tar Cloning ◽  
Cloning Technology

Construction of Human Chromosome 16- and 5-Specific Circular YAC/BAC Libraries byin VivoRecombination in Yeast (TAR Cloning)

Genomics ◽  
1998 ◽  
Vol 53 (1) ◽  
pp. 21-28 ◽  
Author(s):  
N. Kouprina ◽  
M. Campbell ◽  
J. Graves ◽  
E. Campbell ◽  
L. Meincke ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Chromosome 16 ◽  
Bac Libraries ◽  
Tar Cloning

scholarly journals Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology

Chromosoma ◽  
2016 ◽  
Vol 125 (4) ◽  
pp. 621-632 ◽  
Author(s):  
Natalay Kouprina ◽  
Vladimir Larionov
Keyword(s):  
Synthetic Biology ◽  
Tar Cloning
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