Transcription, Reverse Transcription, and Amplification of Backbone‐Modified Nucleic Acids with Laboratory‐Evolved Thermophilic DNA Polymerases

2021 ◽  
Vol 1 (7) ◽  
Author(s):  
Ping Song ◽  
Rujie Zhang ◽  
Chuanping He ◽  
Tingjian Chen
Keyword(s):  
Nucleic Acids ◽  
Reverse Transcription ◽  
Dna Polymerases ◽  
Modified Nucleic Acids

Accurate and Efficient One-Pot Reverse Transcription and Amplification of 2′-Fluoro-Modified Nucleic Acids by Commercial DNA Polymerases

Biochemistry ◽  
2020 ◽  
Vol 59 (31) ◽  
pp. 2833-2841
Author(s):  
Arianna S. Thompson ◽  
Susanna E. Barrett ◽  
Aurora G. Weiden ◽  
Ananya Venkatesh ◽  
Madison K. C. Seto ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Reverse Transcription ◽  
Dna Polymerases ◽  
One Pot ◽  
Modified Nucleic Acids

scholarly journals Transcription of 4′-thioDNA templates to natural RNA in vitro and in mammalian cells

2015 ◽  
Vol 51 (37) ◽  
pp. 7887-7890 ◽  
Author(s):  
Hideto Maruyama ◽  
Kazuhiro Furukawa ◽  
Hiroyuki Kamiya ◽  
Noriaki Minakawa ◽  
Akira Matsuda
Keyword(s):  
Nucleic Acids ◽  
Mammalian Cells ◽  
Genetic Material ◽  
Rna Polymerases ◽  
Chemically Modified ◽  
Biological Studies ◽  
Modified Nucleic Acids

Synthetic chemically modified nucleic acids, which are compatible with DNA/RNA polymerases, have great potential as a genetic material for synthetic biological studies.

scholarly journals Triazole-Modified Nucleic Acids for the Application in Bioorganic and Medicinal Chemistry

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 628
Author(s):  
Dagmara Baraniak ◽  
Jerzy Boryski
Keyword(s):  
Nucleic Acids ◽  
State Of The Art ◽  
Modified Oligonucleotides ◽  
Synthetic Genes ◽  
Chemically Modified ◽  
Amide Linkage ◽  
Dna And Rna ◽  
Modified Dna ◽  
Modified Nucleic Acids ◽  
Non Coding Rnas

This review covers studies which exploit triazole-modified nucleic acids in the range of chemistry and biology to medicine. The 1,2,3-triazole unit, which is obtained via click chemistry approach, shows valuable and unique properties. For example, it does not occur in nature, constitutes an additional pharmacophore with attractive properties being resistant to hydrolysis and other reactions at physiological pH, exhibits biological activity (i.e., antibacterial, antitumor, and antiviral), and can be considered as a rigid mimetic of amide linkage. Herein, it is presented a whole area of useful artificial compounds, from the clickable monomers and dimers to modified oligonucleotides, in the field of nucleic acids sciences. Such modifications of internucleotide linkages are designed to increase the hybridization binding affinity toward native DNA or RNA, to enhance resistance to nucleases, and to improve ability to penetrate cell membranes. The insertion of an artificial backbone is used for understanding effects of chemically modified oligonucleotides, and their potential usefulness in therapeutic applications. We describe the state-of-the-art knowledge on their implications for synthetic genes and other large modified DNA and RNA constructs including non-coding RNAs.

Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection

2015 ◽  
Vol 13 (43) ◽  
pp. 10604-10608 ◽  
Author(s):  
Maëva Reverte ◽  
Jean-Jacques Vasseur ◽  
Michael Smietana
Keyword(s):  
Nucleic Acids ◽  
Boronic Acid ◽  
Enzymatic Degradation ◽  
Label Free ◽  
New Class ◽  
Mismatch Detection ◽  
Modified Dna ◽  
Modified Nucleic Acids

Boronic acid modified DNA emerged as a new class of resistant oligonucleotides against enzymatic degradation. This property has been used to develop an enzyme-assisted label free method for mismatch detection.

scholarly journals Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4659 ◽  
Author(s):  
Steven Ochoa ◽  
Valeria T. Milam
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Chemical Structure ◽  
Chemical Diversity ◽  
Physiochemical Properties ◽  
Phosphate Backbone ◽  
Modified Nucleic Acids ◽  
Recent Developments ◽  
Diagnostic Applications

In the last three decades, oligonucleotides have been extensively investigated as probes, molecular ligands and even catalysts within therapeutic and diagnostic applications. The narrow chemical repertoire of natural nucleic acids, however, imposes restrictions on the functional scope of oligonucleotides. Initial efforts to overcome this deficiency in chemical diversity included conservative modifications to the sugar-phosphate backbone or the pendant base groups and resulted in enhanced in vivo performance. More importantly, later work involving other modifications led to the realization of new functional characteristics beyond initial intended therapeutic and diagnostic prospects. These results have inspired the exploration of increasingly exotic chemistries highly divergent from the canonical nucleic acid chemical structure that possess unnatural physiochemical properties. In this review, the authors highlight recent developments in modified oligonucleotides and the thrust towards designing novel nucleic acid-based ligands and catalysts with specifically engineered functions inaccessible to natural oligonucleotides.

scholarly journals RNA Chemistry for RNA Biology

2019 ◽  
Vol 73 (5) ◽  
pp. 368-373 ◽  
Author(s):  
Pascal Röthlisberger ◽  
Christian Berk ◽  
Jonathan Hall
Keyword(s):  
Chemical Synthesis ◽  
Nucleic Acids ◽  
Chemical Biology ◽  
Rna Synthesis ◽  
Intrinsic Properties ◽  
Chemical Modifications ◽  
Chemically Modified ◽  
Wide Range ◽  
Rna Biology ◽  
Modified Nucleic Acids

Advances in the chemical synthesis of RNA have opened new possibilities to address current questions in RNA biology. Access to site-specifically modified oligoribonucleotides is often a pre-requisite for RNA chemical-biology projects. Driven by the enormous research efforts for development of oligonucleotide therapeutics, a wide range of chemical modifications have been developed to modulate the intrinsic properties of nucleic acids in order to fit their use as therapeutics or research tools. The RNA synthesis platform, supported by the NCCR RNA & Disease, aims to provide access to a large variety of chemically modified nucleic acids. In this review, we describe some of the recent projects that involved work of the platform and highlight how RNA chemistry supports new discoveries in RNA biology.

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