Immunoprecipitation Strategies to Isolate RIPK1/RIPK3 Complexes in Mouse Macrophages

2021 ◽  
Vol 1 (6) ◽  
Author(s):  
Ioannis Siokas ◽  
Dingqiang Zhang ◽  
Alexander Poltorak ◽  
Hayley Muendlein ◽  
Alexei Degterev
Keyword(s):  
Mouse Macrophages

scholarly journals Biochemical and genetic studies of the apoprotein E secreted by mouse macrophages and human monocytes.

1982 ◽  
Vol 257 (16) ◽  
pp. 9788-9795 ◽  
Author(s):  
S K Basu ◽  
Y K Ho ◽  
M S Brown ◽  
D W Bilheimer ◽  
R G Anderson ◽  
...  
Keyword(s):  
Human Monocytes ◽  
Genetic Studies ◽  
Mouse Macrophages ◽  
Apoprotein E

scholarly journals Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nicholas Jones ◽  
Julianna Blagih ◽  
Fabio Zani ◽  
April Rees ◽  
David G. Hill ◽  
...  
Keyword(s):  
Oxidative Metabolism ◽  
Mononuclear Phagocytes ◽  
Alcoholic Fatty Liver ◽  
Lps Challenge ◽  
Fructose Intake ◽  
Mouse Macrophages ◽  
Dietary Fructose ◽  
Monocytes And Macrophages ◽  
Developed World

AbstractFructose intake has increased substantially throughout the developed world and is associated with obesity, type 2 diabetes and non-alcoholic fatty liver disease. Currently, our understanding of the metabolic and mechanistic implications for immune cells, such as monocytes and macrophages, exposed to elevated levels of dietary fructose is limited. Here, we show that fructose reprograms cellular metabolic pathways to favour glutaminolysis and oxidative metabolism, which are required to support increased inflammatory cytokine production in both LPS-treated human monocytes and mouse macrophages. A fructose-dependent increase in mTORC1 activity drives translation of pro-inflammatory cytokines in response to LPS. LPS-stimulated monocytes treated with fructose rely heavily on oxidative metabolism and have reduced flexibility in response to both glycolytic and mitochondrial inhibition, suggesting glycolysis and oxidative metabolism are inextricably coupled in these cells. The physiological implications of fructose exposure are demonstrated in a model of LPS-induced systemic inflammation, with mice exposed to fructose having increased levels of circulating IL-1β after LPS challenge. Taken together, our work underpins a pro-inflammatory role for dietary fructose in LPS-stimulated mononuclear phagocytes which occurs at the expense of metabolic flexibility.

scholarly journals Extracellular ATP perturbs transmembrane ion fluxes, elevates cytosolic [Ca2+], and inhibits phagocytosis in mouse macrophages.

1985 ◽  
Vol 260 (25) ◽  
pp. 13442-13449 ◽  
Author(s):  
S S Sung ◽  
J D Young ◽  
A M Origlio ◽  
J M Heiple ◽  
H R Kaback ◽  
...  
Keyword(s):  
Extracellular Atp ◽  
Ion Fluxes ◽  
Mouse Macrophages

ChemInform Abstract: Beauveriolides, Specific Inhibitors of Lipid Droplet Formation in Mouse Macrophages, Produced by Beauveria sp. FO-6979.

ChemInform ◽  
2010 ◽  
Vol 30 (27) ◽  
pp. no-no
Author(s):  
Ichiji Namatame ◽  
Hiroshi Tomoda ◽  
Shuyi Si ◽  
Yuichi Yamaguchi ◽  
Rokuro Masuma ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipid Droplet Formation ◽  
Mouse Macrophages ◽  
Specific Inhibitors
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