scholarly journals DNA Release from Fe3+-Cross-Linked Alginate Films Triggered by Logically Processed Biomolecular Signals: Integration of Biomolecular Computing and Actuation

ChemPhysChem ◽  
2017 ◽  
Vol 18 (13) ◽  
pp. 1811-1821 ◽  
Author(s):  
Maria Gamella ◽  
Marina Privman ◽  
Saira Bakshi ◽  
Artem Melman ◽  
Evgeny Katz
Keyword(s):  
Biomolecular Computing ◽  
Dna Release

scholarly journals Nuclear Envelope Integrity in Health and Disease: Consequences on Genome Instability and Inflammation

2021 ◽  
Vol 22 (14) ◽  
pp. 7281
Author(s):  
Benoit R. Gauthier ◽  
Valentine Comaills
Keyword(s):  
Nuclear Envelope ◽  
Clinical Symptoms ◽  
Genome Instability ◽  
Chromosomal Rearrangements ◽  
Wide Range ◽  
Complex Chromosomal Rearrangements ◽  
Health And Disease ◽  
Dna Release ◽  
Sting Pathway ◽  
Eukaryote Genome

The dynamic nature of the nuclear envelope (NE) is often underestimated. The NE protects, regulates, and organizes the eukaryote genome and adapts to epigenetic changes and to its environment. The NE morphology is characterized by a wide range of diversity and abnormality such as invagination and blebbing, and it is a diagnostic factor for pathologies such as cancer. Recently, the micronuclei, a small nucleus that contains a full chromosome or a fragment thereof, has gained much attention. The NE of micronuclei is prone to collapse, leading to DNA release into the cytoplasm with consequences ranging from the activation of the cGAS/STING pathway, an innate immune response, to the creation of chromosomal instability. The discovery of those mechanisms has revolutionized the understanding of some inflammation-related diseases and the origin of complex chromosomal rearrangements, as observed during the initiation of tumorigenesis. Herein, we will highlight the complexity of the NE biology and discuss the clinical symptoms observed in NE-related diseases. The interplay between innate immunity, genomic instability, and nuclear envelope leakage could be a major focus in future years to explain a wide range of diseases and could lead to new classes of therapeutics.

Biosensors for Biomolecular Computing: a Review and Future Perspectives

2020 ◽  
Vol 10 (3) ◽  
pp. 554-563
Author(s):  
Simone Aiassa ◽  
Rossana Terracciano ◽  
Sandro Carrara ◽  
Danilo Demarchi
Keyword(s):  
Biomolecular Computing ◽  
Future Perspectives

scholarly journals Biomolecular computing

2002 ◽  
Vol 20 (3) ◽  
pp. 215-216
Author(s):  
Masayuki Yamamura ◽  
Tom Head ◽  
Masami Hagiya
Keyword(s):  
Biomolecular Computing

scholarly journals Extracellular DNA release from the genome-reduced pathogen Mycoplasma hyopneumoniae is essential for biofilm formation on abiotic surfaces

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Benjamin B. A. Raymond ◽  
Cheryl Jenkins ◽  
Lynne Turnbull ◽  
Cynthia B. Whitchurch ◽  
Steven P. Djordjevic
Keyword(s):  
Biofilm Formation ◽  
Mycoplasma Hyopneumoniae ◽  
Extracellular Dna ◽  
Abiotic Surfaces ◽  
Dna Release

scholarly journals Cell-free DNA release by mouse placental explants

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0178845 ◽  
Author(s):  
Mark Phillippe ◽  
Sharareh Adeli
Keyword(s):  
Cell Free Dna ◽  
Free Dna ◽  
Dna Release

scholarly journals The thermodynamics of endosomal escape and DNA release from lipoplexes

2016 ◽  
Vol 18 (4) ◽  
pp. 2591-2596 ◽  
Author(s):  
Yotam Y. Avital ◽  
Niels Grønbech-Jensen ◽  
Oded Farago
Keyword(s):  
Driving Forces ◽  
Endosomal Escape ◽  
Dna Complexes ◽  
Dna Release

In this work we identify and characterize the entropic driving forces governing the process of transfection by lipid–DNA complexes.

Selective DNA Release from DQAsome/DNA Complexes at Mitochondria-Like Membranes

Drug Delivery ◽  
2000 ◽  
Vol 7 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Volkmar Weissig ◽  
Carmen Lizano ◽  
Vladimir P. Torchilin
Keyword(s):  
Dna Complexes ◽  
Dna Release

Monitoring decellularization via absorbance spectroscopy during the derivation of extracellular matrix scaffolds

2021 ◽  
Author(s):  
Camilo Mora-Navarro ◽  
Mario Eduardo Garcia ◽  
Prottasha Sarker ◽  
Emily W Ozpinar ◽  
Jeffrey Enders ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Complex Structure ◽  
Scale Up ◽  
Bioactive Molecules ◽  
Good Representation ◽  
Process Standardization ◽  
Tissue Microenvironment ◽  
Potential Therapeutic Application ◽  
Dna Release

Abstract Extracellular matrix (ECM) is a complex structure composed of bioactive molecules representative of the specific local tissue microenvironment. Decellularized ECM biomaterials harness these biomolecules for regenerative medicine applications. One potential therapeutic application is the use of vocal fold (VF) specific ECM to restore the VFs after injury. ECM scaffolds are derived through a process of decellularization, which aims to remove unwanted immunogenic biomolecules (e.g., DNA) while preserving the composition of the ECM. The effectiveness of the decellularization is typically assessed at the end by quantifying ECM attributes such as final dsDNA content. However, batch-to-batch variability in ECM manufacturing remains a significant challenge for the process standardization, cost-effectiveness, and scale-up. The limited number of tools available for in-process control heavily restricts the uncovering of the correlations between decellularization process parameters and ECM attributes. In this study, we developed a technique applicable to both the classical batch method and semi-continuous decellularization system to trace the decellularization of two laryngeal tissues in real-time. We hypothesize that monitoring the bioreactor's effluent absorbance at 260 nm as a function of time will provide a representative DNA release profile from the tissue and thus allowing for process optimization. The DNA release profiles were obtained for laryngeal tissues and were successfully used to optimize the derivation of VF lamina propria-ECM (auVF-ECM) hydrogels. This hydrogel had comparable rheological properties to commonly used biomaterials to treat VF injuries. Also, the auVF-ECM hydrogel promoted the down-regulation of CCR7 by THP-1 macrophages upon lipopolysaccharide stimulation in vitro suggesting some anti-inflammatory properties. The results show that absorbance profiles are a good representation of DNA removal during the decellularization process thus providing an important tool to optimize future protocols.

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