scholarly journals The thermodynamics of endosomal escape and DNA release from lipoplexes

2016 ◽  
Vol 18 (4) ◽  
pp. 2591-2596 ◽  
Author(s):  
Yotam Y. Avital ◽  
Niels Grønbech-Jensen ◽  
Oded Farago
Keyword(s):  
Driving Forces ◽  
Endosomal Escape ◽  
Dna Complexes ◽  
Dna Release

In this work we identify and characterize the entropic driving forces governing the process of transfection by lipid–DNA complexes.

Selective DNA Release from DQAsome/DNA Complexes at Mitochondria-Like Membranes

Drug Delivery ◽  
2000 ◽  
Vol 7 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Volkmar Weissig ◽  
Carmen Lizano ◽  
Vladimir P. Torchilin
Keyword(s):  
Dna Complexes ◽  
Dna Release

scholarly journals Correction: The thermodynamics of endosomal escape and DNA release from lipoplexes

2016 ◽  
Vol 18 (12) ◽  
pp. 8752-8752
Author(s):  
Yotam Y. Avital ◽  
Niels Grønbech-Jensen ◽  
Oded Farago
Keyword(s):  
Chem Phys ◽  
Endosomal Escape ◽  
Dna Release ◽  
Phys Chem Chem Phys

Correction for ‘The thermodynamics of endosomal escape and DNA release from lipoplexes’ by Yotam Y. Avital et al., Phys. Chem. Chem. Phys., 2016, 18, 2591–2596.

scholarly journals Interaction kinetics of peptide lipids-mediated gene delivery

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Yinan Zhao ◽  
Tianyi Zhao ◽  
Yanyan Du ◽  
Yingnan Cao ◽  
Yang Xuan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Endosomal Escape ◽  
Late Endosomes ◽  
Key Factor ◽  
Interaction Kinetics ◽  
Dna Release

Abstract Background During the course of gene transfection, the interaction kinetics between liposomes and DNA is speculated to play very important role for blood stability, cellular uptake, DNA release and finally transfection efficiency. Results As cationic peptide liposomes exhibited great gene transfer activities both in vitro and in vivo, two peptide lipids, containing a tri-ornithine head (LOrn3) and a mono-ornithine head (LOrn1), were chosen to further clarify the process of liposome-mediated gene delivery in this study. The results show that the electrostatically-driven binding between DNA and liposomes reached nearly 100% at equilibrium, and high affinity of LOrn3 to DNA led to fast binding rate between them. The binding process between LOrn3 and DNA conformed to the kinetics equation: y = 1.663631 × exp (− 0.003427x) + 6.278163. Compared to liposome LOrn1, the liposome LOrn3/DNA lipoplex exhibited a faster and more uniform uptake in HeLa cells, as LOrn3 with a tri-ornithine peptide headgroup had a stronger interaction with the negatively charged cell membrane than LOrn1. The efficient endosomal escape of DNA from LOrn3 lipoplex was facilitated by the acidity in late endosomes, resulting in broken carbamate bonds, as well as the “proton sponge effect” of the lipid. Conclusions The interaction kinetics is a key factor for DNA transfection efficiency. This work provided insights into peptide lipid-mediated DNA delivery that could guide the development of the next generation of delivery systems for gene therapeutics.

CATIONIC POLYMER BASED GENE DELIVERY: UPTAKE AND INTRACELLULAR TRAFFICKING

COSMOS ◽  
2014 ◽  
Vol 10 (01) ◽  
pp. 17-24
Author(s):  
YOONKHEI HO ◽  
HENG-PHON TOO
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Cell Types ◽  
Endosomal Escape ◽  
Current Status ◽  
Uptake Mechanism ◽  
Nuclear Entry ◽  
Dna Complexes ◽  
Major Drawback ◽  
Gene Carriers

To date, low transfection efficiency remains the major drawback of polymer based gene delivery. Many cell types including stem cells, fibroblast and neurons are known to be poorly transfected with polymer based gene carriers and the high toxicity severely restrict their utility in gene delivery. Continual efforts are made to identify cellular barriers to efficient transfection as these carriers have low immunogenicity, ease of manufacturing and scalability. Here, we summarize the current status of understanding on uptake mechanism of polymer-DNA complexes (polyplexes), their endosomal escape, cytosolic transport and nuclear entry of pDNA.

scholarly journals Interaction Kinetics of Peptide Lipids-Mediated Gene Delivery

2019 ◽  
Author(s):  
Shubiao Zhang ◽  
Yinan Zhao ◽  
Yanyan Du ◽  
Yingnan Cao ◽  
Yang Xuan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Endosomal Escape ◽  
Late Endosomes ◽  
Key Factor ◽  
Interaction Kinetics ◽  
Dna Release

Abstract Background: During the course of gene transfection, the interaction kinetics between liposomes and DNA is speculated to play very important role for blood stability, cellular uptake, DNA release and finally transfection efficiency.Results: As cationic peptide liposomes exhibited great gene transfer activities both in vitro and in vivo, two peptide lipids, containing a tri-ornithine head (LOrn3) and a mono-ornithine head (LOrn1), were chosen to further clarify the process of liposome-mediated gene delivery in this study. The results show that the electrostatically-driven binding between DNA and liposomes reached nearly 100% at equilibrium, and high affinity of LOrn3 to DNA led to fast binding rate between them. The binding process between LOrn3 and DNA conformed to the kinetics equation: y = 1.663631 × exp(-0.003427x) + 6.278163. Compared to liposome LOrn1, the liposome LOrn3/DNA lipoplex exhibited a faster and more uniform uptake in Hela cells, as LOrn3 with a tri-ornithine peptide headgroup had a stronger interaction with the negatively charged cell membrane than LOrn1. The efficient endosomal escape of DNA from LOrn3 lipoplexes was facilitated by the acidity in late endosomes, resulting in broken carbamate bonds, as well as the “proton sponge effect” of the lipid.Conclusions: The interaction kinetics is a key factor for DNA transfection efficiency. This work provided insights into peptide lipid-mediated DNA delivery that could guide the development of the next generation of delivery systems for gene therapeutics.

scholarly journals Interaction Kinetics of Peptide Lipids-Mediated Gene Delivery

2020 ◽  
Author(s):  
Yinan Zhao ◽  
Tianyi Zhao ◽  
Yanyan Du ◽  
Yingnan Cao ◽  
Yang Xuan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Endosomal Escape ◽  
Late Endosomes ◽  
Key Factor ◽  
Interaction Kinetics ◽  
Dna Release

Abstract Background: During the course of gene transfection, the interaction kinetics between liposomes and DNA is speculated to play very important role for blood stability, cellular uptake, DNA release and finally transfection efficiency. Results: As cationic peptide liposomes exhibited great gene transfer activities both in vitro and in vivo, two peptide lipids, containing a tri-ornithine head (LOrn3) and a mono-ornithine head (LOrn1), were chosen to further clarify the process of liposome-mediated gene delivery in this study. The results show that the electrostatically-driven binding between DNA and liposomes reached nearly 100% at equilibrium, and high affinity of LOrn3 to DNA led to fast binding rate between them. The binding process between LOrn3 and DNA conformed to the kinetics equation: y = 1.663631 × exp(-0.003427x) + 6.278163. Compared to liposome LOrn1, the liposome LOrn3/DNA lipoplex exhibited a faster and more uniform uptake in HeLa cells, as LOrn3 with a tri-ornithine peptide headgroup had a stronger interaction with the negatively charged cell membrane than LOrn1. The efficient endosomal escape of DNA from LOrn3 lipoplex was facilitated by the acidity in late endosomes, resulting in broken carbamate bonds, as well as the“proton sponge effect”of the lipid. Conclusions: The interaction kinetics is a key factor for DNA transfection efficiency. This work provided insights into peptide lipid-mediated DNA delivery that could guide the development of the next generation of delivery systems for gene therapeutics.

scholarly journals Endosomal escape and transfection efficiency of PEGylated cationic liposome–DNA complexes prepared with an acid-labile PEG-lipid

Biomaterials ◽  
2012 ◽  
Vol 33 (19) ◽  
pp. 4928-4935 ◽  
Author(s):  
Chia-Ling Chan ◽  
Ramsey N. Majzoub ◽  
Rahau S. Shirazi ◽  
Kai K. Ewert ◽  
Yen-Ju Chen ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Cationic Liposome ◽  
Endosomal Escape ◽  
Dna Complexes ◽  
Acid Labile
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