Domain-Specific Folding Kinetics of Staphylococcal Nuclease Observed through Single-Molecule FRET in a Microfluidic Mixer

ChemPhysChem ◽  
2011 ◽  
Vol 12 (18) ◽  
pp. 3515-3518 ◽  
Author(s):  
Zeyong Zhi ◽  
Pengcheng Liu ◽  
Peng Wang ◽  
Yanyi Huang ◽  
Xin Sheng Zhao
Keyword(s):  
Single Molecule ◽  
Staphylococcal Nuclease ◽  
Folding Kinetics ◽  
Microfluidic Mixer ◽  
Domain Specific ◽  
Single Molecule Fret ◽  
Kinetics Of

Ultrafast Microfluidic Mixer for Tracking the Early Folding Kinetics of Human Telomere G-Quadruplex

2014 ◽  
Vol 86 (9) ◽  
pp. 4333-4339 ◽  
Author(s):  
Ying Li ◽  
Chao Liu ◽  
Xiaojun Feng ◽  
Youzhi Xu ◽  
Bi-Feng Liu
Keyword(s):  
Folding Kinetics ◽  
Microfluidic Mixer ◽  
G Quadruplex ◽  
Human Telomere ◽  
Kinetics Of

scholarly journals Probing the kinetic and thermodynamic consequences of the tetraloop/tetraloop receptor monovalent ion-binding site in P4–P6 RNA by smFRET

2015 ◽  
Vol 43 (2) ◽  
pp. 172-178 ◽  
Author(s):  
Namita Bisaria ◽  
Daniel Herschlag
Keyword(s):  
Single Molecule ◽  
Specific Binding ◽  
Specific Interaction ◽  
Monovalent Cations ◽  
Folding Kinetics ◽  
Rna Molecules ◽  
Single Molecule Fret ◽  
Wide Range ◽  
Monovalent Ion

Structured RNA molecules play roles in central biological processes and understanding the basic forces and features that govern RNA folding kinetics and thermodynamics can help elucidate principles that underlie biological function. Here we investigate one such feature, the specific interaction of monovalent cations with a structured RNA, the P4–P6 domain of the Tetrahymena ribozyme. We employ single molecule FRET (smFRET) approaches as these allow determination of folding equilibrium and rate constants over a wide range of stabilities and thus allow direct comparisons without the need for extrapolation. These experiments provide additional evidence for specific binding of monovalent cations, Na+ and K+, to the RNA tetraloop–tetraloop receptor (TL–TLR) tertiary motif. These ions facilitate both folding and unfolding, consistent with an ability to help order the TLR for binding and further stabilize the tertiary contact subsequent to attainment of the folding transition state.

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