Label-Free Measurements of Molecular Transport across Liposome Membranes using Evanescent-Wave Sensing

ChemPhysChem ◽  
2008 ◽  
Vol 9 (17) ◽  
pp. 2480-2485 ◽  
Author(s):  
Magnus Brändén ◽  
Sara Dahlin ◽  
Fredrik Höök
Keyword(s):  
Evanescent Wave ◽  
Molecular Transport ◽  
Label Free

Single Nanoparticle Label-Free Imaging Based on Evanescent Wave In-Plane Scattering

2018 ◽  
Vol 38 (6) ◽  
pp. 0624001
Author(s):  
江丽雯 Jiang Liwen ◽  
孙旭晴 Sun Xuqing ◽  
刘虹遥 Liu Hongyao ◽  
谌雅琴 Chen Yaqin ◽  
熊伟 Xiong Wei ◽  
...  
Keyword(s):  
Evanescent Wave ◽  
Label Free ◽  
Single Nanoparticle

Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280nm wavelength

2011 ◽  
Vol 26 (7) ◽  
pp. 3367-3370 ◽  
Author(s):  
Reshma Bharadwaj ◽  
V.V.R. Sai ◽  
Kamini Thakare ◽  
Arvind Dhawangale ◽  
Tapanendu Kundu ◽  
...  
Keyword(s):  
Evanescent Wave ◽  
Fiber Optic ◽  
Label Free ◽  
E Coli ◽  
Label Free Detection

Label-free fiber optic biosensor based on evanescent wave absorbance at 280nm

2010 ◽  
Vol 143 (2) ◽  
pp. 724-730 ◽  
Author(s):  
V.V.R. Sai ◽  
Tapanendu Kundu ◽  
Chitra Deshmukh ◽  
Susan Titus ◽  
Pradeep Kumar ◽  
...  
Keyword(s):  
Evanescent Wave ◽  
Fiber Optic ◽  
Label Free

scholarly journals Evanescent-Wave Cavity Enhanced Spectroscopy as a Tool in Label-Free Biosensing

2010 ◽  
Author(s):  
L. van der Sneppen ◽  
G. Ritchie ◽  
G. Hancock ◽  
F. Ariese ◽  
C. Gooijer ◽  
...  
Keyword(s):  
Evanescent Wave ◽  
Label Free

Label-Free Optical Method for Quantifying Molecular Transport Across Cellular Membranes In Vitro

2016 ◽  
Vol 7 (17) ◽  
pp. 3406-3411 ◽  
Author(s):  
Mohammad Sharifian Gh. ◽  
Michael J. Wilhelm ◽  
Hai-Lung Dai
Keyword(s):  
Optical Method ◽  
Molecular Transport ◽  
Label Free ◽  
Cellular Membranes

scholarly journals Hartman interferometer: versatile integrated optic sensor for label-free, real-time quantification of nucleic acids, proteins, and pathogens

1997 ◽  
Vol 43 (9) ◽  
pp. 1757-1763 ◽  
Author(s):  
Bernard H Schneider ◽  
John G Edwards ◽  
Nile F Hartman
Keyword(s):  
Real Time ◽  
Optical Sensors ◽  
Evanescent Wave ◽  
Low Cost ◽  
Refractive Index Change ◽  
Polarized Light ◽  
Gene Probe ◽  
Label Free ◽  
Optic Sensor ◽  
Wide Range

Abstract The Hartman interferometer, a proprietary integrated optic sensor, provides a basis for a broad range of biomedical diagnostics, including antibody-based and gene probe-based assays. As with other evanescent-wave optical sensors, the interferometer measures the refractive index change resulting from biomolecular binding on a waveguide surface. The exciting promise of evanescent-wave sensors lies, in general, in their potential to be used as label-free, real-time transducers that can operate in a true mix-and-read fashion and provide fast, quantitative results. One of the major issues facing their development, however, is creating a simple, low-cost configuration for multianalyte testing. The Hartman interferometer addresses this challenge by relying on linearly polarized light and a planar waveguide format, thereby avoiding the problems associatedwith circular polarization and channel waveguides. We report preliminary experiments that demonstrate the applicability of this sensor configuration to detection of a wide range of protein, nucleic acid, and pathogen analytes.

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

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