Two-Color Two-Photon Excitation of Intrinsic Protein Fluorescence: Label-Free Observation of Proteolytic Digestion of Bovine Serum Albumin

ChemPhysChem ◽  
2009 ◽  
Vol 10 (9-10) ◽  
pp. 1607-1613 ◽  
Author(s):  
Stefan Quentmeier ◽  
Claudia C. Quentmeier ◽  
Peter J. Walla ◽  
Karl-Heinz Gericke
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Label Free ◽  
Proteolytic Digestion ◽  
Protein Fluorescence ◽  
Intrinsic Protein ◽  
Two Photon ◽  
Photon Excitation ◽  
Intrinsic Protein Fluorescence

scholarly journals Investigation of two-photon excited fluorescence increment via crosslinked bovine serum albumin

2012 ◽  
Vol 20 (13) ◽  
pp. 13669 ◽  
Author(s):  
Chun-Yu Lin ◽  
Chi-Hsiang Lien ◽  
Keng-Chi Cho ◽  
Chia-Yuan Chang ◽  
Nan-Shan Chang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Two Photon

scholarly journals Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

2011 ◽  
Vol 19 (12) ◽  
pp. 11732 ◽  
Author(s):  
Keng-Chi Cho ◽  
Chi-Hsiang Lien ◽  
Chun-Yu Lin ◽  
Chia-Yuan Chang ◽  
Lynn L. H. Huang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Two Photon

scholarly journals Label-free detection of bovine serum albumin based on an in-fiber Mach-Zehnder interferometric biosensor

2017 ◽  
Vol 25 (15) ◽  
pp. 17105 ◽  
Author(s):  
Zhengyong Li ◽  
Changrui Liao ◽  
Danni Chen ◽  
Jun Song ◽  
Wei Jin ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Label Free ◽  
Label Free Detection ◽  
Interferometric Biosensor

scholarly journals Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 218
Author(s):  
Francesco Arcadio ◽  
Luigi Zeni ◽  
Chiara Perri ◽  
Girolamo D’Agostino ◽  
Giudo Chiaretti ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Limit Of Detection ◽  
Low Cost ◽  
Protein Detection ◽  
Slab Waveguide ◽  
Label Free ◽  
Sensor Configuration ◽  
Application Fields

Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10−9 M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips.

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