scholarly journals Label-free real-time detection of biotinylated bovine serum albumin using a low-cost optical cavity-based biosensor

2018 ◽  
Vol 26 (15) ◽  
pp. 18982 ◽  
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 218
Author(s):  
Francesco Arcadio ◽  
Luigi Zeni ◽  
Chiara Perri ◽  
Girolamo D’Agostino ◽  
Giudo Chiaretti ◽  
...  

Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10−9 M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips.


RSC Advances ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 891-897 ◽  
Author(s):  
Alessandro Sanginario ◽  
Valentina Cauda ◽  
Alberto Bonanno ◽  
Katarzyna Bejtka ◽  
Stefano Sapienza ◽  
...  

We report the fabrication of a customized electronic platform for biosensing, integrating a single functionalized microwire between two gold microelectrodes as a sensing element, including a custom microelectronic chip for signal readout.


2010 ◽  
Vol 26 (5) ◽  
pp. 456-464 ◽  
Author(s):  
Sacha D. Nandlall ◽  
Heiko A. Schiffter ◽  
Sebastian Vonhoff ◽  
Miriam Bazán-Peregrino ◽  
Manish Arora ◽  
...  

2017 ◽  
Vol 25 (15) ◽  
pp. 17105 ◽  
Author(s):  
Zhengyong Li ◽  
Changrui Liao ◽  
Danni Chen ◽  
Jun Song ◽  
Wei Jin ◽  
...  

2020 ◽  
Author(s):  
Xinjian Yin ◽  
Jiaxin Li ◽  
Senhua Chen ◽  
Yuping Wu ◽  
Zhigang She ◽  
...  

<a></a>Here, we report the development of a high-throughput “FP-Tag” OGT assay with bovine serum albumin as a low-cost and superior “FP-Tag”. With this assay, 2-methyleurotinone was identified as a <a></a><a>low </a>M-range OGT inhibitor. This type of assay with BSA as “FP-Tag” would find more applications with other glycosyltransferases.


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