Local intra- and interlaminar connections in mouse barrel cortex

1990 ◽  
Vol 291 (2) ◽  
pp. 231-255 ◽  
Author(s):  
Kerry L. Bernardo ◽  
James S. McCasland ◽  
Thomas A. Woolsey ◽  
Robert N. Strominger
Keyword(s):  
Barrel Cortex ◽  
Interlaminar Connections

Gap junctions in neuro-gliovascular complexes in rat barrel cortex columns

Tsitologiya ◽  
2018 ◽  
Vol 60 (6) ◽  
pp. 448-454
Author(s):  
E. Yu. Kirichenko ◽  
◽  
P.E. Povilaitite ◽  
A.K. Logvinov ◽  
Yu. G. Kirichenko ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Barrel Cortex

Tuning Heterogeneity in Barrel Cortex Improves Sensory Acuity in Behaving Mice

2019 ◽  
Author(s):  
He J.V. Zheng ◽  
Jesse P. Meagher ◽  
Yogi A. Patel ◽  
Hyungbae Kwon
Keyword(s):  
Barrel Cortex ◽  
Sensory Acuity

scholarly journals A simulation study investigating potential diffusion-based MRI signatures of microstrokes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rafat Damseh ◽  
Yuankang Lu ◽  
Xuecong Lu ◽  
Cong Zhang ◽  
Paul J. Marchand ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Statistical Power ◽  
Barrel Cortex ◽  
Signal Loss ◽  
Nuclear Spins ◽  
Directional Diffusion ◽  
Ischemic Tissue ◽  
Significant Difference ◽  
Before And After ◽  
Experimental Validations

AbstractRecent studies suggested that cerebrovascular micro-occlusions, i.e. microstokes, could lead to ischemic tissue infarctions and cognitive deficits. Due to their small size, identifying measurable biomarkers of these microvascular lesions remains a major challenge. This work aims to simulate potential MRI signatures combining arterial spin labeling (ASL) and multi-directional diffusion-weighted imaging (DWI). Driving our hypothesis are recent observations demonstrating a radial reorientation of microvasculature around the micro-infarction locus during recovery in mice. Synthetic capillary beds, randomly- and radially-oriented, and optical coherence tomography (OCT) angiograms, acquired in the barrel cortex of mice (n = 5) before and after inducing targeted photothrombosis, were analyzed. Computational vascular graphs combined with a 3D Monte-Carlo simulator were used to characterize the magnetic resonance (MR) response, encompassing the effects of magnetic field perturbations caused by deoxyhemoglobin, and the advection and diffusion of the nuclear spins. We quantified the minimal intravoxel signal loss ratio when applying multiple gradient directions, at varying sequence parameters with and without ASL. With ASL, our results demonstrate a significant difference (p < 0.05) between the signal-ratios computed at baseline and 3 weeks after photothrombosis. The statistical power further increased (p < 0.005) using angiograms measured at week 4. Without ASL, no reliable signal change was found. We found that higher ratios, and accordingly improved significance, were achieved at lower magnetic field strengths (e.g., B0 = 3T) and shorter echo time TE (< 16 ms). Our simulations suggest that microstrokes might be characterized through ASL-DWI sequence, providing necessary insights for posterior experimental validations, and ultimately, future translational trials.

scholarly journals Loss of Calretinin in L5a impairs the formation of the barrel cortex leading to abnormal whisker-mediated behaviors

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Mingzhao Su ◽  
Junhua Liu ◽  
Baocong Yu ◽  
Kaixing Zhou ◽  
Congli Sun ◽  
...  
Keyword(s):  
Information Integration ◽  
Pyramidal Neurons ◽  
Barrel Cortex ◽  
Sensory Information ◽  
Membrane Excitability ◽  
Novelty Preference ◽  
Layer 4 ◽  
Dendritic Complexity ◽  
Cortex System

AbstractThe rodent whisker-barrel cortex system has been established as an ideal model for studying sensory information integration. The barrel cortex consists of barrel and septa columns that receive information input from the lemniscal and paralemniscal pathways, respectively. Layer 5a is involved in both barrel and septa circuits and play a key role in information integration. However, the role of layer 5a in the development of the barrel cortex remains unclear. Previously, we found that calretinin is dynamically expressed in layer 5a. In this study, we analyzed calretinin KO mice and found that the dendritic complexity and length of layer 5a pyramidal neurons were significantly decreased after calretinin ablation. The membrane excitability and excitatory synaptic transmission of layer 5a neurons were increased. Consequently, the organization of the barrels was impaired. Moreover, layer 4 spiny stellate cells were not able to properly gather, leading to abnormal formation of barrel walls as the ratio of barrel/septum size obviously decreased. Calretinin KO mice exhibited deficits in exploratory and whisker-associated tactile behaviors as well as social novelty preference. Our study expands our knowledge of layer 5a pyramidal neurons in the formation of barrel walls and deepens the understanding of the development of the whisker-barrel cortex system.

scholarly journals Neurovascular coupling during optogenetic functional activation: Local and remote stimulus-response characteristics, and uncoupling by spreading depression

2019 ◽  
Vol 40 (4) ◽  
pp. 808-822 ◽  
Author(s):  
Maximilian Böhm ◽  
David Y Chung ◽  
Carlos A Gómez ◽  
Tao Qin ◽  
Tsubasa Takizawa ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Spreading Depression ◽  
Barrel Cortex ◽  
Disease Process ◽  
Neurovascular Coupling ◽  
Laser Speckle ◽  
Cortical Activation ◽  
Somatosensory Stimulation ◽  
Response Characteristics ◽  
Electrophysiological Activity

Neurovascular coupling is a fundamental response that links activity to perfusion. Traditional paradigms of neurovascular coupling utilize somatosensory stimulation to activate the primary sensory cortex through subcortical relays. Therefore, examination of neurovascular coupling in disease models can be confounded if the disease process affects these multisynaptic pathways. Optogenetic stimulation is an alternative to directly activate neurons, bypassing the subcortical relays. We employed minimally invasive optogenetic cortical activation through intact skull in Thy1-channelrhodopsin-2 transgenic mice, examined the blood flow changes using laser speckle imaging, and related these to evoked electrophysiological activity. Our data show that optogenetic activation of barrel cortex triggers intensity- and frequency-dependent hyperemia both locally within the barrel cortex (>50% CBF increase), and remotely within the ipsilateral motor cortex (>30% CBF increase). Intriguingly, activation of the barrel cortex causes a small (∼10%) but reproducible hypoperfusion within the contralateral barrel cortex, electrophysiologically linked to transhemispheric inhibition. Cortical spreading depression, known to cause neurovascular uncoupling, diminishes optogenetic hyperemia by more than 50% for up to an hour despite rapid recovery of evoked electrophysiological activity, recapitulating a unique feature of physiological neurovascular coupling. Altogether, these data establish a minimally invasive paradigm to investigate neurovascular coupling for longitudinal characterization of cerebrovascular pathologies.

scholarly journals Critical Period Plasticity Is Disrupted in the Barrel Cortex of Fmr1 Knockout Mice

Neuron ◽  
2010 ◽  
Vol 65 (3) ◽  
pp. 385-398 ◽  
Author(s):  
Emily G. Harlow ◽  
Sally M. Till ◽  
Theron A. Russell ◽  
Lasani S. Wijetunge ◽  
Peter Kind ◽  
...  
Keyword(s):  
Knockout Mice ◽  
Critical Period ◽  
Barrel Cortex
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