scholarly journals Developmental characterization of Zswim5 expression in the progenitor domains and tangential migration pathways of cortical interneurons in the mouse forebrain

2020 ◽  
Vol 528 (14) ◽  
pp. 2404-2419
Author(s):  
Chuan‐Chie Chang ◽  
Hsiao‐Ying Kuo ◽  
Shih‐Yun Chen ◽  
Wan‐Ting Lin ◽  
Kuan‐Ming Lu ◽  
...  
Keyword(s):  
Tangential Migration ◽  
Cortical Interneurons ◽  
Migration Pathways

scholarly journals Developmental Characterization of Zswim5 Expression in the Progenitor Domains and Tangential Migration Pathways of Cortical Interneurons in the Mouse Forebrain

2019 ◽  
Author(s):  
Chuan-Chie Chang ◽  
Hsiao-Ying Kuo ◽  
Shih-Yun Chen ◽  
Kuan-Ming Lu ◽  
Weng Lam Fong ◽  
...  
Keyword(s):  
Cell Fate ◽  
Expression Pattern ◽  
Ganglionic Eminence ◽  
Double Labeling ◽  
Tangential Migration ◽  
Cortical Interneurons ◽  
Low Levels ◽  
Migration Pathways

ABSTRACTGABAergic interneurons play an essential role in modulating cortical networks. The progenitor domains of cortical interneurons are localized in developing ventral forebrain, including the medial ganglionic eminence (MGE), caudal ganglionic eminence (CGE), preoptic area (POA) and preoptic hypothalamic border domain (POH). Here, we characterized the expression pattern of Zswim5, an MGE-enriched gene in the mouse forebrain. At E11.5 to E13.5, prominent Zswim5 expression was detected in the subventricular zone (SVZ) of MGE, CGE, POA and POH of ventral telencephalon in which progenitors of cortical interneurons resided. At E15.5 and E17.5, Zswim5 remained detectable in the SVZ of pallidal primordium (MGE). Zswim5 mRNA was markedly decreased after birth and was absent in the adult forebrain. Interestingly, Zswim5 expression pattern resembled the tangential migration pathways of cortical interneurons. Zswim5-positive cells in the MGE appeared to migrate from the MGE through the SVZ of LGE to overlying neocortex. Indeed, Zswim5 was co-localized with Nkx2.1 and Lhx6, markers of progenitos and migratory cortical interneurons. Double labeling showed that Mash1/Ascl1-positive cells did not express Zswim5. Zswim5 expressing cells showed none or at most low levels of Ki67 but co-expressed Tuj1 in the SVZ of MGE. These results suggest that Zswim5 is immediately upregulated as progenitors exiting cell cycle to become postmitotic. Given that recent studies have elucidated that the cell fate of cortical interneurons is determined shortly after postmitotic, the timing of Zswim5 expression in early postmitotic cortical interneurons suggests a potential role of Zswim5 in regulation of neurogenesis and tangential migration of cortical interneurons.

scholarly journals Meningeal defects alter the tangential migration of cortical interneurons in Foxc1hith/hith mice

2012 ◽  
Vol 7 (1) ◽  
pp. 2 ◽  
Author(s):  
Konstantinos Zarbalis ◽  
Youngshik Choe ◽  
Julie A Siegenthaler ◽  
Lori A Orosco ◽  
Samuel J Pleasure
Keyword(s):  
Tangential Migration ◽  
Cortical Interneurons

scholarly journals N-Cadherin Sustains Motility and Polarity of Future Cortical Interneurons during Tangential Migration

2013 ◽  
Vol 33 (46) ◽  
pp. 18149-18160 ◽  
Author(s):  
C. Luccardini ◽  
L. Hennekinne ◽  
L. Viou ◽  
M. Yanagida ◽  
F. Murakami ◽  
...  
Keyword(s):  
Tangential Migration ◽  
Cortical Interneurons

scholarly journals Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies

2015 ◽  
Vol 308 (3) ◽  
pp. C209-C219 ◽  
Author(s):  
Michael W. Nestor ◽  
Samson Jacob ◽  
Bruce Sun ◽  
Deborah Prè ◽  
Andrew A. Sproul ◽  
...  
Keyword(s):  
Pluripotent Stem Cells ◽  
Cell Sorting ◽  
Cortical Development ◽  
Neuronal Cell ◽  
Embryoid Bodies ◽  
Cell Type ◽  
Serum Free ◽  
Human Ipscs ◽  
Cortical Interneurons

Production and isolation of forebrain interneuron progenitors are essential for understanding cortical development and developing cell-based therapies for developmental and neurodegenerative disorders. We demonstrate production of a population of putative calretinin-positive bipolar interneurons that express markers consistent with caudal ganglionic eminence identities. Using serum-free embryoid bodies (SFEBs) generated from human inducible pluripotent stem cells (iPSCs), we demonstrate that these interneuron progenitors exhibit morphological, immunocytochemical, and electrophysiological hallmarks of developing cortical interneurons. Finally, we develop a fluorescence-activated cell-sorting strategy to isolate interneuron progenitors from SFEBs to allow development of a purified population of these cells. Identification of this critical neuronal cell type within iPSC-derived SFEBs is an important and novel step in describing cortical development in this iPSC preparation.

scholarly journals JNK signaling is required for proper tangential migration and laminar allocation of cortical interneurons

Development ◽  
2020 ◽  
Vol 147 (2) ◽  
pp. dev180646 ◽  
Author(s):  
Abigail K. Myers ◽  
Jessica G. Cunningham ◽  
Skye E. Smith ◽  
John P. Snow ◽  
Catherine A. Smoot ◽  
...  
Keyword(s):  
Jnk Signaling ◽  
Tangential Migration ◽  
Cortical Interneurons

scholarly journals Characterization of a Cohort of Patients With LIG4 Deficiency Reveals the Founder Effect of p.R278L, Unique to the Chinese Population

2021 ◽  
Vol 12 ◽  
Author(s):  
Xianze Luo ◽  
Qing Liu ◽  
Jinqiu Jiang ◽  
Wenjing Tang ◽  
Yuan Ding ◽  
...  
Keyword(s):  
Founder Effect ◽  
Genetic Diagnosis ◽  
Primary Immunodeficiency Disease ◽  
Chinese Patients ◽  
Mutation Site ◽  
Ligase Iv ◽  
Immunodeficiency Disease ◽  
Migration Pathways ◽  
Clinical Problems

DNA ligase IV (LIG4) deficiency is an extremely rare autosomal recessive primary immunodeficiency disease caused by mutations in LIG4. Patients suffer from a broad spectrum of clinical problems, including microcephaly, growth retardation, developmental delay, dysmorphic facial features, combined immunodeficiency, and a predisposition to autoimmune diseases and malignancy. In this study, the clinical, molecular, and immunological characteristics of 15 Chinese patients with LIG4 deficiency are summarized in detail. p.R278L (c.833G>T) is a unique mutation site present in the majority of Chinese cases. We conducted pedigree and haplotype analyses to examine the founder effect of this mutation site in China. This suggests that implementation of protocols for genetic diagnosis and for genetic counseling of affected pedigrees is essential. Also, the search might help determine the migration pathways of populations with Asian ancestry.

scholarly journals Vascular-derived SPARC and SerpinE1 regulate interneuron tangential migration and maturation

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Matthieu Genestine ◽  
Daisy Ambriz ◽  
Gregg W Crabtree ◽  
Patrick Dummer ◽  
Anna Molotkova ◽  
...  
Keyword(s):  
Critical Role ◽  
Mouse Development ◽  
Paracrine Factors ◽  
Embryonic Mouse ◽  
Tangential Migration ◽  
Mouse Cortex ◽  
Neuropsychiatric Diseases ◽  
Cortical Interneurons ◽  
Progenitor Domain ◽  
Pre Treatment

Cortical interneurons establish inhibitory microcircuits throughout the neocortex and their dysfunction has been implicated in epilepsy and neuropsychiatric diseases. Developmentally, interneurons migrate from a distal progenitor domain in order to populate the neocortex - a process that occurs at a slower rate in humans than in mice. In this study, we sought to identify factors that regulate the rate of interneuron maturation across the two species. Using embryonic mouse development as a model system, we found that the process of initiating interneuron migration is regulated by blood vessels of the medial ganglionic eminence (MGE), an interneuron progenitor domain. We identified two endothelial cell-derived paracrine factors, SPARC and SerpinE1, that enhance interneuron migration in mouse MGE explants and organotypic cultures. Moreover, pre-treatment of human stem cell-derived interneurons (hSC-interneurons) with SPARC and SerpinE1 prior to transplantation into neonatal mouse cortex enhanced their migration and morphological elaboration in the host cortex. Further, SPARC and SerpinE1-treated hSC-interneurons also exhibited more mature electrophysiological characteristics compared to controls. Overall, our studies suggest a critical role for CNS vasculature in regulating interneuron developmental maturation in both mice and humans.

scholarly journals Chondroitin Sulfate Acts in Concert with Semaphorin 3A to Guide Tangential Migration of Cortical Interneurons in the Ventral Telencephalon

2010 ◽  
Vol 20 (10) ◽  
pp. 2411-2422 ◽  
Author(s):  
Geraldine Zimmer ◽  
Sheine M. Schanuel ◽  
Susanne Bürger ◽  
Franco Weth ◽  
André Steinecke ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Semaphorin 3A ◽  
Ventral Telencephalon ◽  
Tangential Migration ◽  
Cortical Interneurons

scholarly journals Role of CCR8 and Other Chemokine Pathways in the Migration of Monocyte-derived Dendritic Cells to Lymph Nodes

2004 ◽  
Vol 200 (10) ◽  
pp. 1231-1241 ◽  
Author(s):  
Chunfeng Qu ◽  
Emmerson W. Edwards ◽  
Frank Tacke ◽  
Véronique Angeli ◽  
Jaime Llodrá ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lymph Nodes ◽  
Chemokine Receptors ◽  
Human Monocyte ◽  
Monocyte Subsets ◽  
Migration Pathways

Studying the influence of chemokine receptors (CCRs) on monocyte fate may reveal information about which subpopulations of monocytes convert to dendritic cells (DCs) and the migration pathways that they use. First, we examined whether prominent CCRs on different monocyte subsets, CCR2 or CX3CR1, mediated migration events upstream of the accumulation of monocyte-derived DCs in lymph nodes (LNs). Monocytes were labeled and traced by uptake of latex microspheres in skin. Unexpectedly, neither CCR2 nor CX3CR1 were required. However, absence of CCR2 led to an increased labeling of the minor Gr-1int monocyte population, and the number of latex+ DCs that emigrated to LNs was correspondingly increased. Characterization of Gr-1int monocytes revealed that they selectively expressed CCR7 and CCR8 mRNA in blood. CCR7 and CCR8 pathways were used by monocyte-derived DCs during mobilization from skin to LNs. The role of CCR8 in emigration from tissues also applied to human monocyte-derived cells in a model of transendothelial trafficking. Collectively, the data suggest that Gr-1int monocytes may be most disposed to become a lymphatic-migrating DCs. When these monocyte-derived DCs exit skin to emigrate to LNs, they use not only CCR7 but also CCR8, which was not previously recognized to participate in migration to LNs.

Sign in / Sign up

Export Citation Format

Share Document