Sensory cutaneous papillae in the sea lamprey ( Petromyzon marinus L.): II. Ontogeny and immunocytochemical characterization of solitary chemosensory cells

2019 ◽  
Vol 528 (5) ◽  
pp. 865-878
Author(s):  
Tina E. Suntres ◽  
Gheylen Daghfous ◽  
Sirinart Ananvoranich ◽  
Réjean Dubuc ◽  
Barbara S. Zielinski
Keyword(s):  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Solitary Chemosensory Cells

scholarly journals Characterization of Somatically‐Eliminated Genes During Development of the Sea Lamprey (Petromyzon marinus)

2016 ◽  
Vol 33 (9) ◽  
pp. 2337-2344 ◽  
Author(s):  
Stephanie A. Bryant ◽  
Joseph R. Herdy ◽  
Chris T. Amemiya ◽  
Jeramiah J. Smith
Keyword(s):  
Petromyzon Marinus ◽  
Sea Lamprey

Cloning and pharmacological characterization of the neuropeptide Y receptor Y5 in the sea lamprey, Petromyzon marinus

Peptides ◽  
2013 ◽  
Vol 39 ◽  
pp. 64-70 ◽  
Author(s):  
Bo Xu ◽  
Görel Sundström ◽  
Shigehiro Kuraku ◽  
Ingrid Lundell ◽  
Dan Larhammar
Keyword(s):  
Neuropeptide Y ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Pharmacological Characterization ◽  
Neuropeptide Y Receptor

scholarly journals Characterization of a Novel Bile Alcohol Sulfate Released by Sexually Mature Male Sea Lamprey (Petromyzon marinus)

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e68157 ◽  
Author(s):  
Ke Li ◽  
Cory O. Brant ◽  
Michael J. Siefkes ◽  
Hanna G. Kruckman ◽  
Weiming Li
Keyword(s):  
Alcohol Sulfate ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Mature Male ◽  
Bile Alcohol ◽  
Sexually Mature

scholarly journals Functional characterization and osmoregulatory role of the Na+-K+-2Cl− cotransporter in the gill of sea lamprey (Petromyzon marinus), a basal vertebrate

2020 ◽  
Vol 318 (1) ◽  
pp. R17-R29 ◽  
Author(s):  
Ciaran A. Shaughnessy ◽  
Stephen D. McCormick
Keyword(s):  
Petromyzon Marinus ◽  
Functional Characterization ◽  
Sea Lamprey ◽  
Peptide Sequence ◽  
Muscle Water Content ◽  
Muscle Water ◽  
Basal Position ◽  
Salt Secretion

The present study provides molecular and functional characterization of Na+-K+-2Cl− cotransporter (NKCC1/Slc12a2) in the gills of sea lamprey ( Petromyzon marinus), the most basal extant vertebrate with an osmoregulatory strategy. We report the full-length peptide sequence for the lamprey Na-K-Cl cotransporter 1 (NKCC1), which we show groups strongly with and occupies a basal position among other vertebrate NKCC1 sequences. In postmetamorphic juvenile lamprey, nkcc1 mRNA was present in many tissues but was fivefold higher in the gill than any other examined tissue, and NKCC1 protein was only detected in the gill. Gill mRNA and protein abundances of NKCC1 and Na+-K+-ATPase (NKA/Atp1a1) were significantly upregulated (20- to 200-fold) during late metamorphosis in fresh water, coinciding with the development of salinity tolerance, and were upregulated an additional twofold after acclimation to seawater (SW). Immunohistochemistry revealed that NKCC1 in the gill is found in filamental ionocytes coexpressing NKA, which develop during metamorphosis in preparation for SW entry. Lamprey treated with bumetanide, a widely used pharmacological inhibitor of NKCC1, exhibited higher plasma Cl− and osmolality as well as reduced muscle water content after 24 h in SW; there were no effects of bumetanide in freshwater-acclimated lamprey. This work provides the first functional characterization of NKCC1 as a mechanism for branchial salt secretion in lampreys, providing evidence that this mode of Cl− secretion has been present among vertebrates for ~550 million years.

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