Expression of soluble guanylyl cyclase in rat cerebral cortex during postnatal development

2005 ◽  
Vol 485 (3) ◽  
pp. 255-265 ◽  
Author(s):  
Jin-Dong Ding ◽  
Alain Burette ◽  
Richard J. Weinberg
Keyword(s):  
Cerebral Cortex ◽  
Postnatal Development ◽  
Guanylyl Cyclase ◽  
Soluble Guanylyl Cyclase ◽  
Rat Cerebral Cortex

scholarly journals The Expression Pattern of Nitric Oxide-sensitive Guanylyl Cyclase in the Rat Heart Changes During Postnatal Development

2002 ◽  
Vol 50 (10) ◽  
pp. 1325-1331 ◽  
Author(s):  
Sönke Behrends ◽  
Andrea Mietens ◽  
Jörg Kempfert ◽  
Markus Koglin ◽  
Hasso Scholz ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Postnatal Development ◽  
Guanylyl Cyclase ◽  
Rat Heart ◽  
Constitutive Expression ◽  
Soluble Guanylyl Cyclase ◽  
Cell Types ◽  
Perinatal Period ◽  
Dynamic Regulation ◽  
Subsequent Decrease

Nitric oxide (NO)-releasing drugs such as glyceryl trinitrate have been used in the treatment of ischemic heart disease for more than a century. Nevertheless, a detailed analysis of the expression of the NO target enzyme soluble guanylyl cyclase (sGC) in the heart is missing. The aim of the current study was to elucidate the expression, cell distribution, and activity of sGC in the rat heart during postnatal development. Using a novel antibody raised against a C-terminal peptide of the rat β1-subunit of sGC, the enzyme was demonstrated in early postnatal and adult hearts by Western blotting analyses, showing maximal expression in 10-day-old animals. Measurements of basal, NO-, and NO/YC-1-stimulated sGC activity revealed an increase of sGC activity in hearts from neonatal to 10-day-old rats, followed by a subsequent decrease in adult animals. As shown by immunohis-tochemical analysis, sGC expression was present in vascular endothelium and smooth muscle cells in neonatal heart but expression shifted to endothelial cells in adult animals. In isolated cardiomyocytes, sGC activity was not detectable under basal conditions but significant sGC activity could be detected in the presence of NO. An increase in expression during the perinatal period and changes in the cell types expressing sGC at different phases of development suggest dynamic regulation rather than constitutive expression of the NO receptor in the heart.

scholarly journals Postnatal changes in dolichol-pathway enzyme activities in cerebral cortex neurons

1982 ◽  
Vol 202 (1) ◽  
pp. 87-95 ◽  
Author(s):  
V Idoyaga-Vargas ◽  
H Carminatti
Keyword(s):  
Cerebral Cortex ◽  
Postnatal Development ◽  
Enzyme Activities ◽  
Synapse Formation ◽  
Adult Stage ◽  
Protein S ◽  
Rat Cerebral Cortex ◽  
Dolichyl Phosphate ◽  
Endogenous Protein ◽  
Dolichol Pathway

Neuronal perikarya were isolated from rat cerebral cortex at different stages of postnatal development. Membranes sedimenting at 100000 g were obtained from these neurons to study several glycosyltransferases of the dolichol pathway. Enzyme activities from stages before and during synapse formation were compared (days 5 and 15 respectively). Dolichyl diphosphate (Dol-P-P) N-acetylglucosamine, dolichyl phosphate mannose and dolichyl phosphate glucose synthases and the enzymes catalysing Dol-P-P-GlcNAc2Man9Glc3 formation were higher at day 15 of postnatal development. The glycosyl transfer of the latter compound to endogenous protein(s) as well as to a dinitrophenyl-heptapeptide was also measured. The activity was higher at day 15. Furthermore, the activity of dolichyl phosphate mannose synthase was also measured during the time when the number of synapses ceased to increase (day 36) and in the adult stage. The activity of dolichyl phosphate mannose synthase was higher at day 36 than at day 15, and declined in the adult stage. From these results it may be concluded that there is an increase in the glycosylation of asparagine-type glycoproteins during synapse formation in the neurons of the cerebral cortex.

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