Multipotency of purified, transplanted globose basal cells in olfactory epithelium

2004 ◽  
Vol 469 (4) ◽  
pp. 457-474 ◽  
Author(s):  
Xueyan Chen ◽  
Hengsheng Fang ◽  
James E. Schwob
Keyword(s):  
Olfactory Epithelium ◽  
Basal Cells

scholarly journals Zone-specific damage of the olfactory epithelium under protein restriction

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ayinuer Tuerdi ◽  
Shu Kikuta ◽  
Makoto Kinoshita ◽  
Teru Kamogashira ◽  
Kenji Kondo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Olfactory Epithelium ◽  
Control Diet ◽  
Intervention Strategy ◽  
Protein Restriction ◽  
Outer Hair Cells ◽  
Basal Cells ◽  
Cell Dynamics ◽  
Age Related ◽  
Positive Effect

AbstractOxidative stress causes tissue damage, affecting age-related pathologies. Protein restriction (PR) provides a powerful intervention strategy for reducing oxidative stress, which may have a positive effect on individual organs. However, it is unknown whether PR intervention influences the olfactory system. Here, we investigated how 10 months of PR could affect the cell dynamics of the olfactory epithelium (OE) in mice. We found that PR reduced age-related loss of outer hair cells in the cochlea, providing preventive effects against age-related hearing loss. In contrast, PR resulted in reduced mature olfactory sensory neurons (OSNs), increased proliferative basal cells, and increased apoptotic OSNs in zone 1 (the only area containing neurons expressing NQO1 [quinone dehydrogenase 1]) of the OE in comparison with animals given a control diet. Substantial oxidative stress occurred in NQO1-positive cells and induced apoptotic OSNs in zone 1. These results indicate that in contrast to the positive effect on the auditory system, PR induces oxidative stress and structurally and functionally negative effects on OSNs in zone 1, which is probably involved in the bioactivation of NQO1.

Differential and overlapping expression patterns of X-dll3 and Pax-6 genes suggest distinct roles in olfactory system development of the African clawed frog Xenopus laevis

2001 ◽  
Vol 204 (12) ◽  
pp. 2049-2061 ◽  
Author(s):  
Marie-Dominique Franco ◽  
Michael P. Pape ◽  
Jennifer J. Swiergiel ◽  
Gail D. Burd
Keyword(s):  
Xenopus Laevis ◽  
Expression Pattern ◽  
Olfactory Epithelium ◽  
Olfactory System ◽  
De Novo ◽  
System Development ◽  
Expression Patterns ◽  
Basal Cells ◽  
Olfactory Placode ◽  
Water Borne

SUMMARY In Xenopus laevis, the formation of the adult olfactory epithelium involves embryonic, larval and metamorphic phases. The olfactory epithelium in the principal cavity (PC) develops during embryogenesis from the olfactory placode and is thought to respond to water-borne odorants throughout larval life. During metamorphosis, the PC undergoes major transformations and is exposed to air-borne odorants. Also during metamorphosis, the middle cavity (MC) develops de novo. The olfactory epithelium in the MC has the same characteristics as that in the larval PC and is thought to respond to water-borne odorants. Using in situ hybridization, we analyzed the expression pattern of the homeobox genes X-dll3 and Pax-6 within the developing olfactory system. Early in development, X-dll3 is expressed in both the neuronal and non-neuronal ectoderm of the sense plate and in all cell layers of the olfactory placode and larval PC. Expression becomes restricted to the neurons and basal cells of the PC by mid-metamorphosis. During metamorphosis, X-dll3 is also expressed throughout the developing MC epithelium and becomes restricted to neurons and basal cells at metamorphic climax. This expression pattern suggests that X-dll3 is first involved in the patterning and genesis of all cells forming the olfactory tissue and is then involved in neurogenesis or neuronal maturation in putative water- and air-sensing epithelia. In contrast, Pax-6 expression is restricted to the olfactory placode, larval PC and metamorphic MC, suggesting that Pax-6 is specifically involved in the formation of water-sensing epithelium. The expression patterns suggest that X-dll3 and Pax-6 are both involved in establishing the olfactory placode during embryonic development, but subtle differences in cellular and temporal expression patterns suggest that these genes have distinct functions.

scholarly journals Globose basal cells are required for reconstitution of olfactory epithelium after methyl bromide lesion

2003 ◽  
Vol 460 (1) ◽  
pp. 123-140 ◽  
Author(s):  
Woochan Jang ◽  
Steven L. Youngentob ◽  
James E. Schwob
Keyword(s):  
Olfactory Epithelium ◽  
Methyl Bromide ◽  
Basal Cells

scholarly journals Age-Dependent Activation and Neuronal Differentiation of Lgr5+ Basal Cells in Injured Olfactory Epithelium via Notch Signaling Pathway

2020 ◽  
Vol 12 ◽  
Author(s):  
Xuewen Li ◽  
Meimei Tong ◽  
Li Wang ◽  
Yumei Qin ◽  
Hongmeng Yu ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Signaling Pathway ◽  
Sensory Neurons ◽  
Olfactory Epithelium ◽  
Three Dimensional ◽  
Notch Signaling Pathway ◽  
Basal Cells ◽  
Aged Mice ◽  
Age Dependent ◽  
Notch Activation

Aging is an important factor affecting function of smell, leading to the degeneration of mature olfactory sensory neurons and inducing the occurrence of smell loss. The mammalian olfactory epithelium (OE) can regenerate when subjected to chemical assaults. However, this capacity is not limitless. Inactivation of globose basal cells and failure to generate sensory neurons are the main obstacles to prevent the OE regeneration. Here, we found the significant attenuation in mature sensory neuronal generation and apparent transcriptional alternation in the OE from aged mice compared with young ones. The recruitment of leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5)-positive cells in injured OE was weakened in aged mice, and more Lgr5+ cells remained quiescence in aged OE postinjury. Lineage-traced progenies from Lgr5+ cells were significantly fewer in the OE with aging. Moreover, Notch activation enhanced the neuronal regeneration in aged OE, making the regenerative capacity of aged OE comparable with that of young animals after injury. The growth and morphology of three-dimensional (3D)-cultured organoids from the OE of young and aged mice varied and was modulated by small molecules regulating the Notch signaling pathway. Thus, we concluded that activation of Lgr5+ cells in injured OE was age dependent and Notch activation could enhance the capacity of neuronal generation from Lgr5+ cells in aged OE after injury.

scholarly journals Label-retaining, quiescent globose basal cells are found in the olfactory epithelium

2014 ◽  
Vol 522 (4) ◽  
pp. 731-749 ◽  
Author(s):  
Woochan Jang ◽  
Xueyan Chen ◽  
Daniel Flis ◽  
Margaret Harris ◽  
James E. Schwob
Keyword(s):  
Olfactory Epithelium ◽  
Basal Cells

Bulbectomy of neonatal mice induces migration of basal cells from the olfactory epithelium

1998 ◽  
Vol 108 (1-2) ◽  
pp. 295-298 ◽  
Author(s):  
Yuko Suzuki ◽  
Masako Takeda ◽  
Nobuko Obara ◽  
Noriyo Suzuki
Keyword(s):  
Olfactory Epithelium ◽  
Basal Cells ◽  
Neonatal Mice

Observation of basal cells in the olfactory epithelium after axotomy

1990 ◽  
Vol 48 (3) ◽  
pp. 334-335
Author(s):  
Yuko Suzuki ◽  
Masako Takeda
Keyword(s):  
Electron Microscopy ◽  
Olfactory Epithelium ◽  
Structural Changes ◽  
Lamina Cribrosa ◽  
Postnatal Period ◽  
Epithelial Cell Line ◽  
Basal Cells ◽  
Becton Dickinson ◽  
Adult Mice ◽  
Dividing Cells

In the olfactory epithelium of mice, cytokeratin was present in the basal cells but not in the olfactory cells. Our previous study using antikeratin antibodies showed that the basal cells were columnar or pyramidal in shape in the early postnatal period, but became flat in adult mice. In this study, structural changes of the basal cells after axotomy were investigated by immunohistochemistry, transmission electron microscopy (TEM), and scanning electron microscopy (SEM).The unilateral olfactory nerves of adult mice were sectioned at the level of the lamina cribrosa. The mice were sacrificed 4, 8-10, and 14 days postoperatively. The olfactory mucosae were removed, frozen with freon 22, and cut on a cryostat at 10 μm. The sections were stained by the PAP method using a PAP kit. The antikeratin antibody (PKK 2) against pig kidney epithelial cell line, which reacts with 40, 46, 48, and 54 kd subunits (Immunotech), was used. To label the dividing cells, bromodeoxyuridine (BrdU) was administered to mice each postoperative day 1 hour before sacrificing. The presence of BrdU was detected imrnunohistochemically using anti-BrdU antibody (Becton-Dickinson).

scholarly journals Label-retaining, quiescent globose basal cells are found in the olfactory epithelium

2014 ◽  
Vol 522 (4) ◽  
pp. Spc1-Spc1
Author(s):  
Woochan Jang ◽  
Xueyan Chen ◽  
Daniel Flis ◽  
Margaret Harris ◽  
James E. Schwob
Keyword(s):  
Olfactory Epithelium ◽  
Basal Cells

Olfactory epithelium consisting of supporting cells and horizontal basal cells in the posterior nasal cavity of mice

2000 ◽  
Vol 299 (3) ◽  
pp. 313-325 ◽  
Author(s):  
Yuko Suzuki ◽  
Masako Takeda ◽  
Nobuko Obara ◽  
Noriyo Suzuki ◽  
Norihito Takeichi
Keyword(s):  
Nasal Cavity ◽  
Olfactory Epithelium ◽  
Basal Cells ◽  
Supporting Cells ◽  
Horizontal Basal Cells
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