Three-dimensional imaging of the mouse heart and vasculature using micro-CT and whole-body perfusion of iodine or phosphotungstic acid

2014 ◽  
Vol 9 (5) ◽  
pp. 383-390 ◽  
Author(s):  
P. Joy Dunmore-Buyze ◽  
Elsbeth Tate ◽  
Fu-li Xiang ◽  
Sarah A. Detombe ◽  
Zengxuan Nong ◽  
...  
Keyword(s):  
Phosphotungstic Acid ◽  
Three Dimensional ◽  
Whole Body ◽  
Mouse Heart ◽  
Micro Ct ◽  
Three Dimensional Imaging

Three-dimensional imaging of whole mouse models: comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging

2013 ◽  
Vol 253 (1) ◽  
pp. 24-30 ◽  
Author(s):  
A. TAPFER ◽  
M. BECH ◽  
I. ZANETTE ◽  
P. SYMVOULIDIS ◽  
S. STANGL ◽  
...  
Keyword(s):  
Mouse Models ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Micro Ct ◽  
Three Dimensional Imaging ◽  
X Ray

scholarly journals Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Spencer R Katz ◽  
Maksim A Yakovlev ◽  
Daniel J Vanselow ◽  
Yifu Ding ◽  
Alex Y Lin ◽  
...  
Keyword(s):  
High Resolution ◽  
Silver Staining ◽  
Three Dimensional ◽  
Whole Body ◽  
Model Organisms ◽  
Specific Cell ◽  
Micro Ct ◽  
Melanin Content ◽  
Silver Deposition ◽  
X Ray

We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables.

High-Spatial-Resolution Three-dimensional Imaging of Human Spinal Cord and Column Anatomy with Postmortem X-ray Phase-Contrast Micro-CT

Radiology ◽  
2021 ◽  
Vol 298 (1) ◽  
pp. 135-146
Author(s):  
Giacomo E. Barbone ◽  
Alberto Bravin ◽  
Alberto Mittone ◽  
Sergio Grosu ◽  
Jens Ricke ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
High Spatial Resolution ◽  
Three Dimensional ◽  
Micro Ct ◽  
Three Dimensional Imaging ◽  
X Ray ◽  
Human Spinal Cord

scholarly journals Whole-organism 3D Quantitative Characterization of Zebrafish Melanin by Silver Deposition Micro-CT

2021 ◽  
Author(s):  
Spencer R. Katz ◽  
Maksim A. Yakovlev ◽  
Daniel J. Vanselow ◽  
Yifu Ding ◽  
Alex Y. Lin ◽  
...  
Keyword(s):  
Silver Staining ◽  
Skin Color ◽  
Large Scale ◽  
Imaging Modality ◽  
Three Dimensional ◽  
Whole Body ◽  
Model Organisms ◽  
Micro Ct ◽  
Melanin Content ◽  
Silver Deposition

AbstractMelanin-rich zebrafish melanophores are used to study pigment development, human skin color, and as a large-scale screening phenotype. To facilitate more detailed whole-body, computational analyses of melanin content and morphology, we have combined X-ray microtomography (micro-CT), a non-destructive, full-volume imaging modality, with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, for both dramatic and subtle phenotypes not previously described. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, three-dimensional computational phenomic analysis of a particular cell type at cellular resolution, with potential applications in other model organisms and human melanoma biopsies. Whole-organism, high-resolution phenotyping is a challenging ideal, but provides superior context for functional studies of mutations, diseases, and environmental influences.

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