scholarly journals High resolution three‐dimensional imaging and measurement of lung, heart, liver, and diaphragmatic development in the fetal rat based on micro‐computed tomography (micro‐CT)

2020 ◽  
Author(s):  
Moritz Markel ◽  
Marco Ginzel ◽  
Nicole Peukert ◽  
Hartmut Schneider ◽  
Rainer Haak ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Three Dimensional ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Three Dimensional Imaging ◽  
Fetal Rat

MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

2021 ◽  
Author(s):  
Eva Chatzinikolaou ◽  
Kleoniki Keklikoglou
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
3D Imaging ◽  
Imaging Technique ◽  
Ct Scanning ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Gastropod Species ◽  
Biological Studies ◽  
Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

scholarly journals Micro-CT study of Middle Ordovician Spumellaria (radiolarians) from western Newfoundland, Canada

2020 ◽  
Vol 94 (3) ◽  
pp. 417-435 ◽  
Author(s):  
Sarah Kachovich ◽  
Jonathan C. Aitchison
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Detailed Examination ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Lower Paleozoic ◽  
X Ray ◽  
Middle Ordovician ◽  
Radiolarian Assemblage ◽  
Taxonomic Groups

AbstractA new, previously undescribed Middle Ordovician (middle Darriwilian: Dw2) radiolarian assemblage has been recovered from the Table Cove Formation at Piccadilly Quarry, western Newfoundland. Constituents of the fauna described herein are both distinctive and exceptionally well preserved. Three-dimensional X-ray micro-computed tomography (μ-CT) is used to make a detailed examination of four key spumellarian specimens. This technology enables visualization of hitherto ambiguous details of the internal morphologies of key lower Paleozoic taxonomic groups, among which a lack of knowledge has impeded resolution of higher taxonomic rankings.

scholarly journals Three-Dimensional Imaging of Hepatic Sinusoids in Mice Using Synchrotron Radiation Micro-Computed Tomography

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e68600 ◽  
Author(s):  
Yae Jin Yoon ◽  
Soeun Chang ◽  
Oh Youn Kim ◽  
Bo-Kyeong Kang ◽  
Jaesung Park ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Synchrotron Radiation ◽  
Three Dimensional ◽  
Micro Computed Tomography ◽  
Three Dimensional Imaging

High-Resolution Three-Dimensional Micro-Computed Tomography Detects Bone Loss and Changes in Trabecular Architecture Early

2002 ◽  
Vol 37 (1) ◽  
pp. 40-46 ◽  
Author(s):  
ODILE BAROU ◽  
DAVID VALENTIN ◽  
LAURENCE VICO ◽  
CATHERINE TIRODE ◽  
ALAIN BARBIER ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Bone Loss ◽  
Three Dimensional ◽  
Trabecular Architecture ◽  
Micro Computed Tomography

scholarly journals Micro-computed tomography: A novel diagnostic technique for the evaluation of gastrointestinal specimens

2021 ◽  
Vol 09 (12) ◽  
pp. E1886-E1889
Author(s):  
Noboru Kawata ◽  
Alexei Teplov ◽  
Peter Ntiamoah ◽  
Jinru Shia ◽  
Meera Hameed ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Diagnostic Technique ◽  
Human Colon ◽  
Ffpe Tissue ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Formalin Fixed Paraffin Embedded

AbstractMicro-computed tomography (micro-CT) is a non-destructive modality that can be used to obtain high-resolution three-dimensional (3 D) images of the whole sample tissue; the usefulness of micro-CT has been reported for evaluation of breast cancer and lung cancer. However, this novel diagnostic technique has never been used for evaluating endoscopically resected gastrointestinal specimens. In the present study, we scanned 13 formalin-fixed paraffin-embedded (FFPE) tissue blocks of a normal human colon and gastric tissue samples using micro-CT. The evaluation comprised a comparison of the acquired whole block images with the images of the corresponding cross-sectional slice of the hematoxylin and eosin-stained slide. Micro-CT was able to produce images of the whole sample and clearly depict tissues such as glandular structures, muscularis mucosae, and blood vessels in the FFPE tissue blocks of normal gastrointestinal samples. Furthermore, the 3 D reconstructed could be used to create a cross-sectional image and reflected the surface structure of samples obtained from any site. Micro-CT has the potential to become a highly promising pathological diagnostic assistance tool for endoscopically resected gastrointestinal specimens in combination with conventional microscopic examination.

Upscaling Reservoir Rock Porosity by Fractal Dimension Using Three-Dimensional Micro-Computed Tomography and Two-Dimensional Scanning Electron Microscope Images

2020 ◽  
Vol 143 (1) ◽  
Author(s):  
Muhammad Jawad Munawar ◽  
Sandra Vega ◽  
Chengyan Lin ◽  
Mohammad Alsuwaidi ◽  
Naveed Ahsan ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Fractal Dimensions ◽  
Two Dimensional ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Sandstone Sample ◽  
The Core ◽  
Carbonate Sample ◽  
Scanning Electron

Abstract Scaling porosity of sedimentary rocks from the scale of measurement to the scale of interest is still a challenge. Upscaling of porosity can assist to accurately predict other petrophysical properties of rock at multiple scales. In this study, we use the two-dimensional (2D) scanning electron microscope (SEM) and three-dimensional (3D) X-ray micro-computed tomography (micro-CT) image to upscale porosity from the image scale to the core plug scale. A systematic imaging plan is deployed to capture rock properties of a carbonate and a sandstone sample, which are sensitive to the fractal nature of these rocks. Image analysis records wider pore spectrum (0.12–50 µm) in the carbonate sample than in sandstone (0.12–30 µm). The fractal dimensions are also higher in the carbonate than in the sandstone sample. Median, volume-weighted average of pore radius, and fractal dimensions derived from the image analysis are used as inputs in this equation. The results of the present study using this equation yielded to the best results on a resolution of 2.5 µm/voxel in the sandstone and 2.01 µm/voxel resolution in the carbonate sample for 3D micro-CT images, where fractal-scaling porosity matches well with the porosity measured at the core plug scale. The 2D SEM images provided a good estimation of porosity in the sandstone sample, where micro-CT imaging techniques could not capture the full pore spectrum. The fractal porosity equation showed promising results and offers a potential alternative way to estimate porosity when there are no routine core measurements available.

The Location and Incidence of Patent Accessory Pulpal Canals in Permanent Molars with Periodontal Lesion by Using Micro-Computed Tomography

2021 ◽  
Vol 11 (1) ◽  
pp. 85-88
Author(s):  
Dima Chouchi ◽  
Atilla Berberoğlu ◽  
Kaan Orhan ◽  
Ilker Etikan ◽  
Hayriye Tümer ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Periodontal Therapy ◽  
Periodontal Pocket ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Pocket Depth ◽  
Human Teeth ◽  
Similar Region ◽  
Permanent Molars

Objective: In this study, the event of patent accessory pulpal canals situated in periodontal pockets has been resolved to utilize the micro-CT method. Methods: Fifty-seven extracted permanent human teeth were utilized to examine the commonness, location, and diameter of the patent accessory canals that are opening outside toward the periodontal pocket by utilizing micro-CT and high-resolution desktop. Results: Through 57 molars 6 (10.25%) were accessible with patent accessory, canals inside furcation area, of root, 49 (85%) were accessible with patent accessory canals inside the mesial and distal buccal root, and 28 (49.1%) were accessible with patent accessory, canals inside the palatal/lingual root. A measurably critical variety was found alongside the nearness of patent accessory trench in various roots and pocket depth at a similar region of the nearness waterway just as the nearness of these canals and connection misfortune in this region. Conclusions: Progression of periodontal pocket might be responsible for opening accessory canal and cause pulp pathosis, making successful periodontal therapy difficult.

scholarly journals Evaluation of scaffold microstructure and comparison of cell seeding methods using micro-computed tomography-based tools

2020 ◽  
Vol 17 (165) ◽  
pp. 20200102
Author(s):  
Aleksi Palmroth ◽  
Sanna Pitkänen ◽  
Markus Hannula ◽  
Kaarlo Paakinaho ◽  
Jari Hyttinen ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Adipose Derived Stem Cells ◽  
Micro Ct ◽  
Cell Seeding ◽  
Micro Computed Tomography ◽  
Tissue Engineering Scaffolds ◽  
Scaffold Architecture ◽  
Static Seeding

Micro-computed tomography (micro-CT) provides a means to analyse and model three-dimensional (3D) tissue engineering scaffolds. This study proposes a set of micro-CT-based tools firstly for evaluating the microstructure of scaffolds and secondly for comparing different cell seeding methods. The pore size, porosity and pore interconnectivity of supercritical CO 2 processed poly( l -lactide-co- ɛ -caprolactone) (PLCL) and PLCL/β-tricalcium phosphate scaffolds were analysed using computational micro-CT models. The models were supplemented with an experimental method, where iron-labelled microspheres were seeded into the scaffolds and micro-CT imaged to assess their infiltration into the scaffolds. After examining the scaffold architecture, human adipose-derived stem cells (hASCs) were seeded into the scaffolds using five different cell seeding methods. Cell viability, number and 3D distribution were evaluated. The distribution of the cells was analysed using micro-CT by labelling the hASCs with ultrasmall paramagnetic iron oxide nanoparticles. Among the tested seeding methods, a forced fluid flow-based technique resulted in an enhanced cell infiltration throughout the scaffolds compared with static seeding. The current study provides an excellent set of tools for the development of scaffolds and for the design of 3D cell culture experiments.

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