Synthesis and cellular uptake of a MR contrast agent coupled to an antisense peptide nucleic acid - cell- penetrating peptide conjugate

2007 ◽  
Vol 2 (1) ◽  
pp. 42-49 ◽  
Author(s):  
W. Su ◽  
R. Mishra ◽  
J. Pfeuffer ◽  
K.-H. Wiesmüller ◽  
K. Ugurbil ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Contrast Agent ◽  
Cellular Uptake ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Antisense Peptide

scholarly journals Cooperative Cellular Uptake and Activity of Octaarginine Antisense Peptide Nucleic acid (PNA) Conjugates

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 554 ◽  
Author(s):  
Ghavami ◽  
Shiraishi ◽  
Nielsen
Keyword(s):  
Cellular Uptake ◽  
Peptide Nucleic Acid ◽  
Peptide Nucleic Acids ◽  
Endosomal Escape ◽  
Cell Penetrating Peptide ◽  
Uptake Mechanism ◽  
Common Effect ◽  
Cell Penetrating ◽  
Antisense Peptide ◽  
Endosomal Uptake

Cellular uptake and antisense activity of d-octaarginine conjugated peptide nucleic acids (PNAs) is shown to exhibit pronounced cooperativity in serum-containing medium, in particular by being enhanced by analogous mis-match PNA–cell-penetrating peptide (PNA–CPP) conjugates without inherent antisense activity. This cooperativity does not show cell or PNA sequence dependency, suggesting that it is a common effect in cationic CPP conjugated PNA delivery. Interestingly, our results also indicate that Deca-r8-PNA and r8-PNA could assist each other and even other non-CPP PNAs as an uptake enhancer agent. However, the peptide itself (without being attached to the PNA) failed to enhance uptake and antisense activity. These results are compatible with an endosomal uptake mechanism in which the endocytosis event is induced by multiple CPP–PNA binding to the cell surface requiring a certain CPP density, possibly in terms of nanoparticle number and/or size, to be triggered. In particular the finding that the number of endosomal events is dependent on the total CPP–PNA concentration supports such a model. It is not possible from the present results to conclude whether endosomal escape is also cooperatively induced by CPP–PNA.

scholarly journals Inhibition of telomerase activity by a cell-penetrating peptide nucleic acid construct in human melanoma cells

FEBS Letters ◽  
2000 ◽  
Vol 473 (2) ◽  
pp. 241-248 ◽  
Author(s):  
Raffaella Villa ◽  
Marco Folini ◽  
Susanna Lualdi ◽  
Silvio Veronese ◽  
Maria Grazia Daidone ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Telomerase Activity ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Cell Penetrating Peptide ◽  
Human Melanoma Cells ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Therapeutic effects, immunogenicity and cytotoxicity of a cell penetrating peptide-peptide nucleic acid conjugate against cagA of Helicobacter pylori in cell culture and animal model

2021 ◽  
Author(s):  
Narges Nodeh Farahani ◽  
Behrooz Sadeghi Kalani ◽  
Seyed Hamidreza Monavari ◽  
Shiva Mirkalantari ◽  
Fatemeh Montazer ◽  
...  
Keyword(s):  
Cell Culture ◽  
Animal Model ◽  
Helicobacter Pylori ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Mrna Levels ◽  
Cell Penetrating ◽  
A Cell ◽  
H Pylori

Background and Objectives: Helicobacter pylori causes several gastrointestinal diseases, including asymptomatic gastritis, chronic peptic ulcer, duodenal ulcer, lymphoma of the mucosa-associated lymphoid tissue (MALT), and gastric adenocarcinoma. In recent years, failure to eradicate H. pylori infections has become an alarming problem for physicians. It is now clear that the current treatment strategies may become ineffective, necessitating the development of innovative antimicrobial compounds as alternative treatments. Materials and Methods: In this experimental study, a cell-penetrating peptide-conjugated peptide nucleic acid (CPP-PNA) was used to target the cagA expression. cagA expression was evaluated using RT-qPCR assay after treatment by the CPPPNA in cell culture and animal model. Additionally, immunogenicity and toxicity of the CPP-PNA were assessed in both cell culture and animal models. Results: Our analysis showed that cagA mRNA levels reduced in H. pylori-infected HT29 cells after treatment with CPPPNA in a dose-dependent manner. Also, cagA expression in bacterial RNA extracted from stomach tissue of mice treated with PNA was reduced compared to that of untreated mice. The expression of inflammatory cytokines also decreased in cells and tissue of H. pylori-infected mice after PNA treatment. The tested CPP-PNA showed no significant adverse effects on cell proliferation of cultured cells and no detectable toxicity and immunogenicity were observed in mice. Conclusion: These results suggest the effectiveness of CPP-PNA in targeting CagA for various research and therapeutic purposes, offering a potential antisense therapy against H. pylori infections.

scholarly journals Evaluation of cell-penetrating peptide–peptide nucleic acid effect in the inhibition of cagA in Helicobacter pylori

2020 ◽  
pp. 1-7
Author(s):  
Zahra Javanmard ◽  
Behrooz Sadeghi Kalani ◽  
Shabnam Razavi ◽  
Narges Nodeh Farahani ◽  
Rokhsareh Mohammadzadeh ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Acid Effect ◽  
Cell Penetrating
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