Enhanced delivery of cell-penetrating peptide–peptide nucleic acid conjugates by endosomal disruption

2006 ◽  
Vol 1 (2) ◽  
pp. 633-636 ◽  
Author(s):  
Takehiko Shiraishi ◽  
Peter E Nielsen
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

scholarly journals Inhibition of telomerase activity by a cell-penetrating peptide nucleic acid construct in human melanoma cells

FEBS Letters ◽  
2000 ◽  
Vol 473 (2) ◽  
pp. 241-248 ◽  
Author(s):  
Raffaella Villa ◽  
Marco Folini ◽  
Susanna Lualdi ◽  
Silvio Veronese ◽  
Maria Grazia Daidone ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Telomerase Activity ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Cell Penetrating Peptide ◽  
Human Melanoma Cells ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Therapeutic effects, immunogenicity and cytotoxicity of a cell penetrating peptide-peptide nucleic acid conjugate against cagA of Helicobacter pylori in cell culture and animal model

2021 ◽  
Author(s):  
Narges Nodeh Farahani ◽  
Behrooz Sadeghi Kalani ◽  
Seyed Hamidreza Monavari ◽  
Shiva Mirkalantari ◽  
Fatemeh Montazer ◽  
...  
Keyword(s):  
Cell Culture ◽  
Animal Model ◽  
Helicobacter Pylori ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Mrna Levels ◽  
Cell Penetrating ◽  
A Cell ◽  
H Pylori

Background and Objectives: Helicobacter pylori causes several gastrointestinal diseases, including asymptomatic gastritis, chronic peptic ulcer, duodenal ulcer, lymphoma of the mucosa-associated lymphoid tissue (MALT), and gastric adenocarcinoma. In recent years, failure to eradicate H. pylori infections has become an alarming problem for physicians. It is now clear that the current treatment strategies may become ineffective, necessitating the development of innovative antimicrobial compounds as alternative treatments. Materials and Methods: In this experimental study, a cell-penetrating peptide-conjugated peptide nucleic acid (CPP-PNA) was used to target the cagA expression. cagA expression was evaluated using RT-qPCR assay after treatment by the CPPPNA in cell culture and animal model. Additionally, immunogenicity and toxicity of the CPP-PNA were assessed in both cell culture and animal models. Results: Our analysis showed that cagA mRNA levels reduced in H. pylori-infected HT29 cells after treatment with CPPPNA in a dose-dependent manner. Also, cagA expression in bacterial RNA extracted from stomach tissue of mice treated with PNA was reduced compared to that of untreated mice. The expression of inflammatory cytokines also decreased in cells and tissue of H. pylori-infected mice after PNA treatment. The tested CPP-PNA showed no significant adverse effects on cell proliferation of cultured cells and no detectable toxicity and immunogenicity were observed in mice. Conclusion: These results suggest the effectiveness of CPP-PNA in targeting CagA for various research and therapeutic purposes, offering a potential antisense therapy against H. pylori infections.

scholarly journals Evaluation of cell-penetrating peptide–peptide nucleic acid effect in the inhibition of cagA in Helicobacter pylori

2020 ◽  
pp. 1-7
Author(s):  
Zahra Javanmard ◽  
Behrooz Sadeghi Kalani ◽  
Shabnam Razavi ◽  
Narges Nodeh Farahani ◽  
Rokhsareh Mohammadzadeh ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Acid Effect ◽  
Cell Penetrating

Activatable Cell Penetrating Peptide–Peptide Nucleic Acid Conjugate via Reduction of Azobenzene PEG Chains

2014 ◽  
Vol 136 (37) ◽  
pp. 12868-12871 ◽  
Author(s):  
Soo Hyeon Lee ◽  
Elena Moroz ◽  
Bastien Castagner ◽  
Jean-Christophe Leroux
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Acid Conjugate

Cell-Penetrating Peptide-Mediated Delivery of Peptide Nucleic Acid (PNA) Oligomers

2008 ◽  
Vol 2008 (4) ◽  
pp. pdb.prot4889-pdb.prot4889
Author(s):  
P. Lundberg ◽  
K. Kilk ◽  
U. Langel
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

Characteristics of Cell-Penetrating Peptide/Nucleic Acid Nanoparticles

2015 ◽  
Vol 13 (1) ◽  
pp. 172-179 ◽  
Author(s):  
Helerin Margus ◽  
Piret Arukuusk ◽  
Ülo Langel ◽  
Margus Pooga
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating
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