Long-term observation of cultured cells by interference-reflection microscopy: Near-infrared illumination and Y-contrast image processing

1989 ◽  
Vol 13 (2) ◽  
pp. 94-103 ◽  
Author(s):  
Martin S. Zand ◽  
Guenter Albrecht-Buehler
Keyword(s):  
Image Processing ◽  
Near Infrared ◽  
Cultured Cells ◽  
Long Term Observation ◽  
Contrast Image

Long-term observation of cultured cells by interference-reflection microscopy: near infrared illumination and Y-contrast image processing

1988 ◽  
Vol 46 ◽  
pp. 70-71
Author(s):  
M. S. Zand ◽  
G. Albrecht-Buehler
Keyword(s):  
Image Processing ◽  
Near Infrared ◽  
Cultured Cells ◽  
Video Recording ◽  
Time Lapse ◽  
Live Cells ◽  
3 Dimensional ◽  
Long Term Observation ◽  
Contrast Image

Analysis of dynamic changes in cell-substratum adhesion patterns during cell locomotion requires continuous, extended observation of single living cells. To date, interference-reflection microscopy (IRM) is the only method available to visualize cell -substratum adhesions in vitro. This method uses 1% of the incident illumination to produce an IRM image, and so far requires use of a high intensity visible light source (400 - 800 nm). However, light of this intensity and spectral range induces marked changes in fibroblast behavior, including cessation of locomotion. Therefore, we developed a method allowing continuous IRM observation of live cells for up to 8 hours, without any observable changes in normal cell behavior, using near infrared illumination (750-1100 nm). In addition, we use Y-contrast image processing of IRM images to create a 3-dimensional relief of the ventral cell surface.Single locomoting PTK1 cells were observed continuously in IRM with time lapse video recording for periods of up to 8 hours.

scholarly journals Use of Autologous Epithelium Transplantation on Various Scaffolds to Cover Tissue Loss in Oral Cavity: Long-Term Observation

2017 ◽  
Vol 15 (1) ◽  
pp. 25-30
Author(s):  
Bogusława Orzechowska-Wylęgała ◽  
Dariusz Dobrowolski ◽  
Domenico Puzzolo ◽  
Bogumił Wowra ◽  
Wiktor Niemiec ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Oral Cavity ◽  
Amniotic Membrane ◽  
Cultured Cells ◽  
Single Layer ◽  
Tissue Loss ◽  
Mucous Membranes ◽  
Well Differentiated ◽  
Long Term Observation

Background The aim of this study was to investigate the application of mucous membrane keratinocyte cultures on amniotic membrane and on poly(L-lactic acid) (PLLA) Purasorb PL38 to cover tissue loss in the oral cavity. Developments in molecular biology techniques and tissue engineering allow the culturing and identification of cells that can be anchored in the wound to achieve integrity of the tissue. Transplantation of tissues obtained from the patient's own cells is superior to allogenous transplantation where there is a possibility of transfection, rejection and the need for long-term immunosuppression. Methods In 9 patients (15 procedures) keratinocytes cultured on amniotic membrane and PLLA were transplanted to cover antro-oral fistulas and bone loss after osteoradionecrosis. Results In all 6 patients with outlasting antro-oral fistulas, the defects were healed. In 3 patients with 5 cases of tissue loss after osteoradionecrosis, we obtained healing of the wound in only 1 case. Histological examination of the cultures indicated that cultured cells formed well-differentiated layers, very similar to the keratinocytes of mucous membranes, although those seeded on amniotic membrane formed a single layer of cells, while those seeded on the PLLA scaffold were arranged on 2 or more layers: these differences were shown to be statistically significant with a morphometric analysis. Conclusions Autologous transplants of epithelium cultured on amniotic membrane and PLLA constitute a new and effective way of covering nonhealing tissue loss in the oral cavity in chosen cases, using modern methods of tissue engineering.

Association of Serum PCSK9 with Near-Infrared Spectroscopy Derived Lipid Core Burden Index and Long-Term Cardiac Outcome

2019 ◽  
Author(s):  
Sharda S. Anroedh ◽  
Rohit M. Oemrawsingh ◽  
Robert-Jan van Geuns ◽  
Jin M. Cheng ◽  
Hector M. Garcia-Garcia ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Lipid Core ◽  
Cardiac Outcome

scholarly journals SAT0137 Long-term observation of fibromyalgia patients

2001 ◽  
Author(s):  
L Szczepanski ◽  
C Stepniak ◽  
B Targonska-Stepniak
Keyword(s):  
Long Term Observation

scholarly journals Phenotype of the Aging-Dependent Spontaneous Onset of Hearing Loss in DBA/2 Mice

2021 ◽  
Vol 8 (3) ◽  
pp. 49
Author(s):  
Min-Soo Seo ◽  
Byeonghyeon Lee ◽  
Kyung-Ku Kang ◽  
Soo-Eun Sung ◽  
Joo-Hee Choi ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Early Stage ◽  
Auditory Brainstem ◽  
Dependent Manner ◽  
Intrinsic Properties ◽  
Hearing Ability ◽  
Brainstem Response ◽  
Long Term Observation ◽  
Spontaneous Onset

DBA/2 mice are a well-known animal model for hearing loss developed due to intrinsic properties of these animals. However, results on the phenotype of hearing loss in DBA/2 mice have been mainly reported at an early stage in mice aged ≤7 weeks. Instead, the present study evaluated the hearing ability at 5, 13, and 34 weeks of age using DBA/2korl mice. Auditory brainstem response test was performed at 8–32 KHz at 5, 13, and 34 weeks of age, and hearing loss was confirmed to be induced in a time-dependent manner. In addition, histopathological evaluation at the same age confirmed the morphological damage of the cochlea. The findings presented herein are the results of the long-term observation of the phenotype of hearing loss in DBA/2 mice and can be useful in studies related to aging-dependent hearing loss.

scholarly journals Posterior Uveal Effusion after Cataract Surgery

2021 ◽  
pp. 62-67
Author(s):  
Annegret Abaza ◽  
Özlem Dikmetas ◽  
Irmingard Neuhann ◽  
Faik Gelisken
Keyword(s):  
Cataract Surgery ◽  
Corticosteroid Therapy ◽  
Anterior Segment ◽  
Subretinal Fluid ◽  
Posterior Pole ◽  
Functional Improvement ◽  
Long Term Observation ◽  
The Right ◽  
Uveal Effusion

We report a case of posterior uveal effusion (UE) with a long-term follow-up that has occurred following cataract surgery. A 64-year-old woman presented with diminished vision of the right eye (RE) 3 weeks after an uneventful phacoemulsification and intraocular lens implantation. Complete ophthalmic examination including fluorescein angiography (FA), indocyanine green angiography (ICGA), echography and optical coherence tomography (OCT) were performed. Best corrected visual acuity (BCVA) of the RE was 20/50. Anterior segment and intraocular pressure were unremarkable. OCT revealed prominent folds of the choroid and retina, subretinal fluid and darkening of the choroid with reduced visibility of the choroidal vessels and the scleral border. The left eye (LE) was unremarkable. BCVA of the LE was: 20/20. After topical anti-inflammatory and systemic corticosteroid therapy for 5 months, no morphological change of the macula was seen. The patient was observed without any treatment. Forty-three months after the cataract surgery and 38 months after cessation of the corticosteroid therapy, OCT revealed a normal macular morphology and the BCVA improved to 20/25. Even though rare, UE at the posterior pole may occur after modern cataract surgery. OCT examination is a reliable tool in monitoring the macular morphology. Since morphological and functional improvement can be seen in long-term, observation may be considered for some cases of posterior UE with resistance to the therapy.

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