Isolation and characterization of sea urchin egg spectrin: Calcium modulation of the spectrin-actin interaction

1987 ◽  
Vol 7 (4) ◽  
pp. 304-314 ◽  
Author(s):  
Douglas J. Fishkind ◽  
Edward M. Bonder ◽  
David A. Begg
Keyword(s):  
Sea Urchin ◽  
Calcium Modulation ◽  
Isolation And Characterization ◽  
Sea Urchin Egg

scholarly journals Isolation and characterization of sea urchin egg cortical granules.

1982 ◽  
Vol 95 (3) ◽  
pp. 924-932 ◽  
Author(s):  
G S Kopf ◽  
G W Moy ◽  
V D Vacquier
Keyword(s):  
Plasma Membrane ◽  
Sea Urchin ◽  
Specific Activity ◽  
Specific Radioactivity ◽  
Marker Enzyme ◽  
Cortical Granules ◽  
Electrophoretic Patterns ◽  
Isolation And Characterization ◽  
Sea Urchin Egg

A method has been developed to isolate cortical granules (CG) free in suspension. It involves the mechanical disruption of the CG from CG lawns (CGL; Dev. Biol. 43:62-74, 1975) and concentration of the CG by low speed centrifugation. The isolated CG are intact and are a relatively pure population as judged by electron microscopy. Granule integrity is confirmed by the fact that isolated intact CG are radioiodinated to only 0.05% of the specific activity of hypotonically lysed CG. Purity of the CG preparation is assessed by the enrichment (four- to sevenfold) of CG marker enzymes and the absence or low activity of plasma membrane, mitochondrial, cytoplasmic, and yolk platelet marker enzyme activities. CG isolated from 125I-surface-labeled eggs have a very low specific radioactivity, demonstrating that CG contamination by the plasma membrane-vitelline layer (PM-VL) is minimal. CG yield is approximately 1% of the starting egg protein. The CG isolation method is simple and rapid, 4 mg of CG protein being obtained in 1 h. Isolated CG and PM-VL display distinct electrophoretic patterns on SDS gels. Actin is localized to the PM-VL, and all bands present in the CGL are accounted for in the CG and PM-VL. Calmodulin is associated with the CGL, CG, and PM-VL fractions, but is not specifically enriched in these fractions as compared with whole egg homogenates. This method of isolating intact CG from unfertilized sea urchin eggs may be useful for exploring the mechanism of Ca2+-mediated CG exocytosis.

Characterization of the sea urchin egg receptor for sperm

1996 ◽  
Vol 24 (1) ◽  
pp. 100S-100S
Author(s):  
Kay Ohlendieck ◽  
William J. Lennarz
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Egg

Characterization of cortical secretory vesicles from the sea urchin egg

1983 ◽  
Vol 96 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Susan J. Decker ◽  
William H. Kinsey
Keyword(s):  
Sea Urchin ◽  
Secretory Vesicles ◽  
Sea Urchin Egg

Isolation and Characterization of Low Density Detergent-Insoluble Membrane (LD-DIM) Fraction from Sea Urchin Sperm

1999 ◽  
Vol 258 (3) ◽  
pp. 616-623 ◽  
Author(s):  
Kaoru Ohta ◽  
Chihiro Sato ◽  
Tsukasa Matsuda ◽  
Masaru Toriyama ◽  
William J. Lennarz ◽  
...  
Keyword(s):  
Sea Urchin ◽  
Low Density ◽  
Isolation And Characterization ◽  
Sea Urchin Sperm

Isolation and characterization of sea urchin toxin

Toxicon ◽  
1965 ◽  
Vol 3 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Charles B. Alender ◽  
George A. Feigen ◽  
Joseph T. Tomita
Keyword(s):  
Sea Urchin ◽  
Isolation And Characterization
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