Isolation and characterization of a new class of acidic glycans implicated in sea urchin embryonal cell adhesion

1993 ◽  
Vol 53 (2) ◽  
pp. 98-113 ◽  
Author(s):  
Eleni Papakonstantinou ◽  
Gradimir N. Misevic
Keyword(s):  
Cell Adhesion ◽  
Sea Urchin ◽  
New Class ◽  
Isolation And Characterization

Isolation and Characterization of Low Density Detergent-Insoluble Membrane (LD-DIM) Fraction from Sea Urchin Sperm

1999 ◽  
Vol 258 (3) ◽  
pp. 616-623 ◽  
Author(s):  
Kaoru Ohta ◽  
Chihiro Sato ◽  
Tsukasa Matsuda ◽  
Masaru Toriyama ◽  
William J. Lennarz ◽  
...  
Keyword(s):  
Sea Urchin ◽  
Low Density ◽  
Isolation And Characterization ◽  
Sea Urchin Sperm

scholarly journals Isolation and characterization of mutants that produce the allantoin-degrading enzymes constitutively in Saccharomyces cerevisiae.

1982 ◽  
Vol 2 (9) ◽  
pp. 1088-1095 ◽  
Author(s):  
G Chisholm ◽  
T G Cooper
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzyme Synthesis ◽  
Common Element ◽  
Transport Systems ◽  
New Class ◽  
Degrading Enzymes ◽  
Isolation And Characterization ◽  
Nitrogen Repression ◽  
Internal Induction

Degradation of allantoin, allantoate, or urea by Saccharomyces cerevisiae requires the participation of four enzymes and four transport systems. Production of the four enzymes and one of the active transport systems is inducible; allophanate, the last intermediate of the pathway, functions as the inducer. The involvement of allophanate in the expression of five distinct genes suggested that they might be regulated by a common element. This suggestion is now supported by the isolation of a new class of mutants (dal80). Strains possessing lesions in the DAL80 locus produce the five inducible activities at high, constitutive levels. Comparable constitutive levels of activity were also observed in doubly mutant strains (durl dal80) which are unable to synthesize allophanate. This, with the observation that arginase activity remained at its uninduced, basal level in strains mutated at the DAL80 locus, eliminates internal induction as the basis for constitutive enzyme synthesis. Mutations in dal80 are recessive to wild-type alleles. The DAL80 locus has been located and is not linked to any of the structural genes of the allantoin pathway. Synthesis of the five enzymes produced constitutively in dal80-1-containing mutants remains normally sensitive to nitrogen repression even though the dal80-1 mutation is present. From these observations we conclude that production of the allantoin-degrading enzymes is regulated by the DAL80 gene product and that induction and repression of enzyme synthesis can be cleanly separated mutationally.

scholarly journals ISOLATION AND CHARACTERIZATION OF C-TYPE VIRAL GENE PRODUCTS OF VIRUS-NEGATIVE MOUSE CELLS

1974 ◽  
Vol 139 (2) ◽  
pp. 427-438 ◽  
Author(s):  
John R. Stephenson ◽  
Steven R. Tronick ◽  
Roberta K. Reynolds ◽  
Stuart A. Aaronson
Keyword(s):  
Viral Gene ◽  
Gene Products ◽  
New Class ◽  
Viral Antigens ◽  
Isolation And Characterization ◽  
Cell Extracts ◽  
Mouse Cells ◽  
Specific Antigens ◽  
Low Levels

Antigens which immunologically cross-react with two mouse C-type viral polypeptides, p30 and p12, are present at very low levels in normal virus-negative mouse cells. These two antigens have been purified by 50–300-fold from cell extracts and shown to cochromatograph with the corresponding labeled viral polypeptides in several systems. Their type-specific antigenicities are shown to be distinct from those of previously tested MuLV isolates suggesting that they may be components of a new class of endogenous C-type virus. The methods utilized in the present studies for concentration of virus-specific antigens of normal mouse cells provide an approach for detection of C-type viral antigens in cells of other species.

Isolation and characterization of sea urchin toxin

Toxicon ◽  
1965 ◽  
Vol 3 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Charles B. Alender ◽  
George A. Feigen ◽  
Joseph T. Tomita
Keyword(s):  
Sea Urchin ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of two forms of a cytoskeleton.

1979 ◽  
Vol 83 (1) ◽  
pp. 109-115 ◽  
Author(s):  
K T Edds
Keyword(s):  
Sea Urchin ◽  
Electron Micrographs ◽  
Actin Filaments ◽  
Three Dimensional ◽  
Strongylocentrotus Droebachiensis ◽  
Major Protein ◽  
Molecular Weights ◽  
Isolation And Characterization ◽  
Filament Bundles

Isolated petaloid coelomocytes from the sea urchin Strongylocentrotus droebachiensis transform to a filopodial morphology in hypotonic media. Electron micrographs of negatively stained Triton-insoluble cytoskeletons show that the petaloid form consists of a loose net of microfilaments while the filopodial form consists of paracrystalline bundles of microfilaments. Actin is the major protein of both forms of the cytoskeleton. Additional polypeptides have molecular weights of approximately 220,000, 64,000, 57,000, and 27,000 daltons. Relative to actin the filopodial cytoskeletons have an average of 2.5 times as much 57k polypeptide as the petaloid cytoskeletons. Treatment with 0.25 M NaCl dissociates the filament bundles into individual actin filaments free of the actin-associated polypeptides. Thus, one or more of these actin-associated polypeptides may be responsible for crosslinking the actin filaments into bundles and maintaining the three-dimensional nature of the cytoskeletons.

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