scholarly journals Actin cytoskeleton in cell polarity and asymmetric division during mouse oocyte maturation

Cytoskeleton ◽  
2012 ◽  
Vol 69 (10) ◽  
pp. 727-737 ◽  
Author(s):  
Kexi Yi ◽  
Rong Li
Keyword(s):  
Actin Cytoskeleton ◽  
Cell Polarity ◽  
Oocyte Maturation ◽  
Asymmetric Division ◽  
Mouse Oocyte

scholarly journals Tropomodulin-3 is essential in asymmetric division during mouse oocyte maturation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yu-Jin Jo ◽  
Woo-In Jang ◽  
Nam-Hyung Kim ◽  
Suk Namgoong
Keyword(s):  
Oocyte Maturation ◽  
Asymmetric Division ◽  
Mouse Oocyte

mInscuteable regulates meiotic spindle organization during mouse oocyte meiotic maturation

Zygote ◽  
2019 ◽  
Vol 28 (1) ◽  
pp. 45-50
Author(s):  
Zhuoni Xiao ◽  
Jiali Peng ◽  
Meiting Xie ◽  
Jing Yang ◽  
Wangming Xu
Keyword(s):  
Oocyte Maturation ◽  
Germinal Vesicle ◽  
Asymmetric Division ◽  
Mouse Oocyte ◽  
Meiotic Spindle ◽  
Meiotic Maturation ◽  
Cellular Polarity ◽  
Germinal Vesicle Breakdown ◽  
Key Events ◽  
Oocyte Meiotic Maturation

SummaryEstablishment of cellular polarity is one of the key events during oocyte maturation. Inscuteable (Insc) has been identified as a key regulator of cell polarity during asymmetric division in Drosophila. However, the function of its evolutionarily conserved mammalian homologue, mInscuteable (mInsc), in mouse meiotic maturation is not clear. In this study, we investigated the roles of mInsc in mouse oocyte maturation. mInsc was detected at all stages of oocyte maturation. The protein level of mInsc was slightly higher at the germinal vesicle breakdown (GVBD) stage and remained constant during mouse oocyte maturation. The subcellular localization of mInsc overlapped with spindle microtubules. Disruption of microtubules and microfilaments caused changes in the localization of mInsc. Depletion or overexpression of mInsc significantly decreased the maturation rates of mouse oocytes. Depletion of mInsc significantly affected asymmetric division, spindle assembly, alignments of chromosomes and actin cap formation. Taken together, our results demonstrated that mInsc regulates meiotic spindle organization during mouse meiotic maturation.

scholarly journals RAB14 GTPase is essential for actin‐based asymmetric division during mouse oocyte maturation

2021 ◽  
Author(s):  
Yuan‐Jing Zou ◽  
Meng‐Meng Shan ◽  
Hong‐Hui Wang ◽  
Zhen‐Nan Pan ◽  
Meng‐Hao Pan ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Asymmetric Division ◽  
Mouse Oocyte

Discs large homologue 1 (Dlg1) coordinates mouse oocyte polarisation during maturation

2017 ◽  
Vol 29 (9) ◽  
pp. 1699
Author(s):  
Jun-Chao Wang ◽  
Hong Lv ◽  
Ke-Liang Wu ◽  
Yun-Shan Zhang ◽  
Hai-Ning Luo ◽  
...  
Keyword(s):  
Cell Polarity ◽  
Oocyte Maturation ◽  
Culture Medium ◽  
Cytochalasin B ◽  
Germinal Vesicle ◽  
Mouse Oocyte ◽  
Meiotic Spindle ◽  
Vesicle Membrane ◽  
Discs Large ◽  
Oocyte Cytoplasm

Mouse oocyte meiotic division requires the establishment of asymmetries in the oocyte before division, indicating the presence of polarity-establishing molecules. During mouse oocyte maturation proper orientation and positioning of the meiotic spindle at the oocyte cortex, as well as polarity in the oocyte cytoplasm and its oolemma, are necessary for the formation of functional haploid oocytes. Discs large homologue 1 (Dlg1) protein is a conserved protein that regulates cell polarity. In the present study, we found that Dlg1 was expressed at different stages of oocyte development. The localisation of Dlg1 during mouse oocyte maturation and its relationship with the cytoskeleton were analysed. Our data show that at the germinal vesicle stage, Dlg1 was present in the cytoplasm, prominently surrounding the germinal vesicle membrane. During maturation, Dlg1 became highly polarised by associating with the spindle and formed characteristic crescent-shaped accumulations under the cortex. Addition of nocodazole or cytochalasin B into the culture medium at different stages changed the localisation of Dlg1, indicating that the organisation of Dlg1 is a complex multi-step process and is dependent on microtubules and microfilaments. More importantly, we found that silencing of Dlg1 compromised the G2–M transition.

PLCz-induced Ca2+ oscillations are enhanced after germinal vesicle breakdown during mouse oocyte maturation

2016 ◽  
Author(s):  
Jessica Sanders ◽  
Ethan Bateson ◽  
Yuansong Yu ◽  
Michail Nomikos ◽  
Antony Lai ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Germinal Vesicle ◽  
Mouse Oocyte ◽  
Germinal Vesicle Breakdown

Rho-GTPase effector ROCK regulates mouse oocyte maturation and embryo development

2014 ◽  
Author(s):  
Xing Duan ◽  
Zhen-Bo Wang ◽  
Xiang-Shun Cui ◽  
Nam-Hyung Kim ◽  
Shao-Chen Sun
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Rho Gtpase ◽  
Mouse Oocyte

scholarly journals Ral GTPase is essential for actin dynamics and Golgi apparatus distribution in mouse oocyte maturation

Cell Division ◽  
2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Ming-Hong Sun ◽  
Lin-Lin Hu ◽  
Chao-Ying Zhao ◽  
Xiang Lu ◽  
Yan-Ping Ren ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Oocyte Maturation ◽  
Polar Body ◽  
Mouse Oocyte ◽  
Actin Dynamics ◽  
Mouse Oocytes ◽  
Oocyte Meiosis ◽  
Ral Gtpase ◽  
Polar Body Extrusion ◽  
Cytoskeleton Dynamics

Abstract Background Ral family is a member of Ras-like GTPase superfamily, which includes RalA and RalB. RalA/B play important roles in many cell biological functions, including cytoskeleton dynamics, cell division, membrane transport, gene expression and signal transduction. However, whether RalA/B involve into the mammalian oocyte meiosis is still unclear. This study aimed to explore the roles of RalA/B during mouse oocyte maturation. Results Our results showed that RalA/B expressed at all stages of oocyte maturation, and they were enriched at the spindle periphery area after meiosis resumption. The injection of RalA/B siRNAs into the oocytes significantly disturbed the polar body extrusion, indicating the essential roles of RalA/B for oocyte maturation. We observed that in the RalA/B knockdown oocytes the actin filament fluorescence intensity was significantly increased at the both cortex and cytoplasm, and the chromosomes were failed to locate near the cortex, indicating that RalA/B regulate actin dynamics for spindle migration in mouse oocytes. Moreover, we also found that the Golgi apparatus distribution at the spindle periphery was disturbed after RalA/B depletion. Conclusions In summary, our results indicated that RalA/B affect actin dynamics for chromosome positioning and Golgi apparatus distribution in mouse oocytes.

scholarly journals MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity

2017 ◽  
Vol 10 (503) ◽  
pp. eaan3286 ◽  
Author(s):  
María-José Sandí ◽  
Christopher B. Marshall ◽  
Marc Balan ◽  
Étienne Coyaud ◽  
Ming Zhou ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Cell Polarity
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