Rho-GTPase effector ROCK regulates mouse oocyte maturation and embryo development

2014 ◽  
Author(s):  
Xing Duan ◽  
Zhen-Bo Wang ◽  
Xiang-Shun Cui ◽  
Nam-Hyung Kim ◽  
Shao-Chen Sun
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Rho Gtpase ◽  
Mouse Oocyte

scholarly journals Leptin and ObRa/MEK signalling in mouse oocyte maturation and preimplantation embryo development

2009 ◽  
Vol 19 (2) ◽  
pp. 181-190 ◽  
Author(s):  
Yinghui Ye ◽  
Kazuhiro Kawamura ◽  
Mitsue Sasaki ◽  
Nanami Kawamura ◽  
Peter Groenen ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Preimplantation Embryo ◽  
Mouse Oocyte ◽  
Preimplantation Embryo Development

scholarly journals UCH-L1 inhibitor LDN-57444 hampers mouse oocyte maturation by regulating oxidative stress and mitochondrial function and reducing ERK1/2 expression

2020 ◽  
Vol 40 (10) ◽  
Author(s):  
Pan Yuan ◽  
Li Zhou ◽  
Xiaona Zhang ◽  
Lan Yao ◽  
Jun Ning ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Mitochondrial Function ◽  
Molecular Mechanisms ◽  
Polar Body ◽  
Mouse Oocyte ◽  
Body Formation ◽  
First Polar Body ◽  
Spindle Body

Abstract Oocyte maturation is a prerequisite for successful fertilization and embryo development. Incomplete oocyte maturation can result in infertility. Ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) has been found to be implicated in oocyte maturation and embryo development. However, the cellular and molecular mechanisms of UCH-L1 underlying oocyte maturation have not been fully elucidated. In the present study, we observed that the introduction of UCH-L1 inhibitor LDN-57444 suppressed first polar body extrusion during mouse oocyte maturation. The inhibition of UCH-L1 by LDN-57444 led to the notable increase in reactive oxygen species (ROS) level, conspicuous reduction in glutathione (GSH) content and mitochondrial membrane potential (MMP), and blockade of spindle body formation. As a conclusion, UCH-L1 inhibitor LDN-57444 suppressed mouse oocyte maturation by improving oxidative stress, attenuating mitochondrial function, curbing spindle body formation and down-regulating extracellular signal-related kinases (ERK1/2) expression, providing a deep insight into the cellular and molecular basis of UCH-L1 during mouse oocyte maturation.

Absence of mitochondrial DNA methylation in mouse oocyte maturation, aging and early embryo development

2019 ◽  
Vol 513 (4) ◽  
pp. 912-918 ◽  
Author(s):  
Li-Hua Fan ◽  
Zhen-Bo Wang ◽  
Qian-Nan Li ◽  
Tie-Gang Meng ◽  
Ming-Zhe Dong ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mitochondrial Dna ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Early Embryo ◽  
Mouse Oocyte ◽  
Early Embryo Development

The role of propofol on mouse oocyte meiotic maturation and early embryo development

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 261-269
Author(s):  
Xia-Guang Duan ◽  
Zai-Qing Huang ◽  
Chun-Guang Hao ◽  
Xiao-Jun Zhi ◽  
Xiao-Bing Qi ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Polar Body ◽  
Oocyte Retrieval ◽  
Early Embryo ◽  
Mouse Oocyte ◽  
Early Embryo Development ◽  
Intravenous Anaesthetic ◽  
Polar Body Extrusion

SummaryPropofol is a intravenous anaesthetic most commonly used in ultrasound oocyte retrieval. We studied if the use of propofol had an effect on mouse oocyte maturation, pregnancy, childbirth and progeny and investigated the correlation between propofol side effects and reproductive performance in mice. There was no statistical difference in mating, pregnancy, childbirth, litter size, the number of stillbirths and survival between each group (P>0.05). Propofol also had no effect on polar body extrusion in oocyte maturation as well as on pronucleus formation and, subsequently, early embryo development (P>0.05). An increased concentration of propofol had no effect on this result, although propofol at more than 0.01 mg/ml reduced polar body extrusion. Different concentrations of propofol had no effect on oocyte culture in vitro, pronucleus formation and early embryo development.

scholarly journals Chk2 Regulates Cell Cycle Progression during Mouse Oocyte Maturation and Early Embryo Development

2014 ◽  
Vol 37 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Xiao-Xin Dai ◽  
Xing Duan ◽  
Hong-Lin Liu ◽  
Xiang-Shun Cui ◽  
Nam-Hyung Kim ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Cell Cycle Progression ◽  
Early Embryo ◽  
Mouse Oocyte ◽  
Cycle Progression ◽  
Early Embryo Development
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