Homogeneous DNA Detection Based on Fluorescence Quenching by Nanoparticles in Single-step Format: Target-Induced Configuration Transform

2009 ◽  
Vol 27 (3) ◽  
pp. 523-528 ◽  
Author(s):  
Songbai ZHANG ◽  
Zaisheng WU ◽  
Ming XIE ◽  
Guoli SHEN ◽  
Ruqin YU
Keyword(s):  
Fluorescence Quenching ◽  
Dna Detection ◽  
Single Step

A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 4019-4025 ◽  
Author(s):  
Fulin Zhou ◽  
Baoxin Li ◽  
Jiyuan Ma
Keyword(s):  
Fluorescence Quenching ◽  
Molecular Beacon ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Dna Probes ◽  
Dna Probe ◽  
Rolling Circle ◽  
Hairpin Probe ◽  
Linear Dna ◽  
Signal Probe

Linear single-labeled DNA probes are used in this RCA-based fluorescence strategy for DNA detection, which could effectively avoid the fluorescence quenching between neighboring signal probes using hairpin probe as signal probe.

Development of a single-step immunoassay microdevice based on a graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions

The Analyst ◽  
2017 ◽  
Vol 142 (3) ◽  
pp. 472-477 ◽  
Author(s):  
Akihiro Shirai ◽  
Kaho Nakashima ◽  
Kenji Sueyoshi ◽  
Tatsuro Endo ◽  
Hideaki Hisamoto
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Single Step ◽  
Size Separation ◽  
Microchannel Array ◽  
Pdms Microchannel ◽  
Peg Coating ◽  
Separation Functions

An immunoassay was successfully integrated into a “single-step” by developing a microdevice composed of a GO-containing hydrogel and a PDMS microchannel array with a PEG coating containing a fluorescently-labelled antibody.

Fluorescence quenching of quantum dots by gold nanorods and its application to DNA detection

2009 ◽  
Vol 94 (6) ◽  
pp. 063111 ◽  
Author(s):  
Xin Li ◽  
Jun Qian ◽  
Li Jiang ◽  
Sailing He
Keyword(s):  
Quantum Dots ◽  
Fluorescence Quenching ◽  
Gold Nanorods ◽  
Dna Detection

scholarly journals Single step for Neisseria gonorrhoeae genomic DNA detection by using gold nanoparticle probe.

2019 ◽  
Vol 30 (2) ◽  
Author(s):  
Paweena Tunakhun ◽  
Pornsuda Maraming ◽  
Ratree Tavichakorntrakool ◽  
Phitsamai Saisud ◽  
Saksit Sungkiri ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Gold Nanoparticle ◽  
Genomic Dna ◽  
Dna Detection ◽  
Single Step

Fast and single-step immunoassay based on fluorescence quenching within a square glass capillary immobilizing graphene oxide–antibody conjugate and fluorescently labelled antibody

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3389-3394 ◽  
Author(s):  
Akihiro Shirai ◽  
Terence G. Henares ◽  
Kenji Sueyoshi ◽  
Tatsuro Endo ◽  
Hideaki Hisamoto
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Single Step ◽  
Glass Capillary ◽  
Antibody Conjugate ◽  
Ultrafast Measurement

A single-step immunoassay device for ultrafast measurement of proteins within 40 seconds, fabricated by using a square glass capillary, antibody–graphene oxide conjugate and fluorescently labelled antibody, was developed.

A novel label-free strategy for pathogenic DNA detection based on metal ion binding-induced fluorescence quenching of graphitic carbon nitride nanosheets

The Analyst ◽  
2017 ◽  
Vol 142 (14) ◽  
pp. 2617-2623 ◽  
Author(s):  
Yan Xiao ◽  
Yuhao Sheng ◽  
Jie Zhou ◽  
Miaomiao Chen ◽  
Wei Wen ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Metal Ions ◽  
Metal Ion ◽  
Carbon Nitride ◽  
Dna Detection ◽  
Ion Binding ◽  
Label Free ◽  
Metal Ion Binding ◽  
Fluorescence Sensing ◽  
Carbon Nitride Nanosheets

A novel label-free fluorescence sensing strategy based on g-C3N4 nanosheets and metal ions is designed for pathogenic DNA detection.

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