Enhancement of bioconversion gangliosides to monosialotetrahexosylganglioside by in situ sialic acid removal and recovery

2019 ◽  
Vol 97 (10) ◽  
pp. 2617-2620 ◽  
Author(s):  
Yansong Song ◽  
Xuedong Wang ◽  
Jianguo Zhang
Keyword(s):  
Sialic Acid ◽  
Removal And Recovery

scholarly journals Exo-enzymatic addition of diazirine-modified sialic acid to cell surfaces enables photocrosslinking of glycoproteins

2021 ◽  
Author(s):  
Nageswari Yarravarapu ◽  
Rohit Sai Reddy Konada ◽  
Narek Darabedian ◽  
Nichole J. Pedowtiz ◽  
Soumya N. Krishnamurthy ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Cell Types ◽  
Cell Surfaces ◽  
Binding Interactions ◽  
Multiple Cell ◽  
Labeling Method ◽  
Cell Surface Glycoconjugates

Glycan binding often mediates extracellular macromolecular recognition events. Accurate characterization of these binding interactions can be difficult because of dissociation and scrambling that occur during purification and analysis steps. Use of photocrosslinking methods has been pursued to covalently capture glycan-dependent interactions in situ however use of metabolic glycan engineering methods to incorporate photocrosslinking sugar analogs is limited to certain cell types. Here we report an exo-enzymatic labeling method to add a diazirine-modified sialic acid (SiaDAz) to cell surface glycoconjugates. The method involves chemoenzymatic synthesis of diazirine-modified CMP-sialic acid (CMP-SiaDAz), followed by sialyltransferase-catalyzed addition of SiaDAz to desialylated cell surfaces. Cell surface SiaDAz-ylation is compatible with multiple cell types and is facilitated by endogenous extracellular sialyltransferase activity present in Daudi B cells. This method for extracellular addition of α2-6-linked SiaDAz enables UV-induced crosslinking of CD22, demonstrating the utility for covalent capture of glycan-mediated binding interactions.

Enzyme-catalyzed synthesis of sialyl oligosaccharide with in situ regeneration of CMP-sialic acid

1991 ◽  
Vol 113 (12) ◽  
pp. 4698-4700 ◽  
Author(s):  
Yoshitaka Ichikawa ◽  
G. J. Shen ◽  
C. H. Wong
Keyword(s):  
Sialic Acid ◽  
In Situ Regeneration

Concomitant release of sialic acids and calcium by neuraminidase from rat aorta in situ

1988 ◽  
Vol 235 (1279) ◽  
pp. 139-144 ◽  
Keyword(s):  
Sialic Acid ◽  
Wistar Rats ◽  
Rat Aorta ◽  
Sialic Acids ◽  
Molar Ratio ◽  
Male Wistar Rats

Male Wistar rats were heparinized and killed with pentobarbital. The upper and lower ends of the aortae were cannulated and the blood was washed out with saline until the washings contained calcium and sialic-acid-reacting material at minimal concentrations. The aortae were perfused with neuraminidase for 15 min. This caused the appearance of calcium as well as of sialic acids in the perfusate in total amounts of about 5.3 nmol and about 3.6 nmol per aorta respectively. The molar ratio of about 1.5 is sufficiently close to that determined for the association of calcium with sialic acids in vitro to suggest that their association is similar in vivo .

scholarly journals SUGGESTIONS FOR A HISTOCHEMICAL TERMINOLOGY OF CARBOHYDRATE-RICH TISSUE COMPONENTS

1965 ◽  
Vol 13 (7) ◽  
pp. 599-603 ◽  
Author(s):  
S. S. SPICER ◽  
T. J. LEPPI ◽  
P. J. STOWARD
Keyword(s):  
Sialic Acid ◽  
Connective Tissue ◽  
Acid Mucopolysaccharide ◽  
Tissue Sections ◽  
Histochemical Methods ◽  
General Terms

Biochemically known mucopolysaccharides can rarely be identified unequivocally in specific tisse sites by histochemical methods. Moreover, many carbohydrate-rich substances that have been detected histochemically (in terms of their vic-glycol content and characteristic acid groups) cannot be related to mucopolysaccharides and glycoproteins known to biochemists. Accordingly, a terminology and classification is proposed for delineating mucosubstances in situ in terms of their histochemical properties. Mucosubstance and mucosaccharide are recommended as general terms for tissue entities rich in carbohydrate, but the term [acid] mucopolysaccharide is restricted to biochemically known mucopolysaccharides of connective tissue. The terms sulfomucin and sialomucin are suggested for acidic epithelial mucosubstances containing sulfate esters and sialic acid, respectively. Neutral mucosubstance is reserved for naming periodate-reactive entities, except glycogen, that lack demonstrable acid groups. The various muscosubstances that have been found in tissue sections so far are tabulated with suggested names.

Rapid removal and recovery of emulsified oil from ASP produced water using in situ formed magnesium hydroxide

2018 ◽  
Vol 4 (4) ◽  
pp. 539-548 ◽  
Author(s):  
Wenjing Dong ◽  
Dejun Sun ◽  
Yujiang Li ◽  
Tao Wu
Keyword(s):  
Magnesium Hydroxide ◽  
Produced Water ◽  
Oil Removal ◽  
Promising Technology ◽  
Emulsified Oil ◽  
Rapid Removal ◽  
Removal And Recovery

In situ formed magnesium hydroxide (IFM) can be used as a promising technology for emulsified oil removal and recovery.

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