Enzyme-catalyzed synthesis of sialyl oligosaccharide with in situ regeneration of CMP-sialic acid

1991 ◽  
Vol 113 (12) ◽  
pp. 4698-4700 ◽  
Author(s):  
Yoshitaka Ichikawa ◽  
G. J. Shen ◽  
C. H. Wong
Keyword(s):  
Sialic Acid ◽  
In Situ Regeneration

scholarly journals In situ regeneration of bioactive coatings enabled by an evolved Staphylococcus aureus sortase A

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Hyun Ok Ham ◽  
Zheng Qu ◽  
Carolyn A. Haller ◽  
Brent M. Dorr ◽  
Erbin Dai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sortase A ◽  
Bioactive Coatings ◽  
In Situ Regeneration

In situ regeneration of commercial NH3-SCR catalysts with high-temperature water vapor

2018 ◽  
Vol 116 ◽  
pp. 57-61 ◽  
Author(s):  
Yao Shi ◽  
Pei Zhang ◽  
Tuotuo Fang ◽  
Erhao Gao ◽  
Fujuan Xi ◽  
...  
Keyword(s):  
Water Vapor ◽  
High Temperature ◽  
Nh3 Scr ◽  
High Temperature Water ◽  
In Situ Regeneration ◽  
Scr Catalysts ◽  
Temperature Water

scholarly journals In-situ regeneration of activated carbon with electric potential swing desorption (EPSD) for the H2S removal from biogas

2018 ◽  
Vol 249 ◽  
pp. 125-131 ◽  
Author(s):  
M. Farooq ◽  
M.N. Almustapha ◽  
M. Imran ◽  
M.A. Saeed ◽  
John M. Andresen
Keyword(s):  
Activated Carbon ◽  
Electric Potential ◽  
H2s Removal ◽  
In Situ Regeneration

scholarly journals Exo-enzymatic addition of diazirine-modified sialic acid to cell surfaces enables photocrosslinking of glycoproteins

2021 ◽  
Author(s):  
Nageswari Yarravarapu ◽  
Rohit Sai Reddy Konada ◽  
Narek Darabedian ◽  
Nichole J. Pedowtiz ◽  
Soumya N. Krishnamurthy ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Cell Types ◽  
Cell Surfaces ◽  
Binding Interactions ◽  
Multiple Cell ◽  
Labeling Method ◽  
Cell Surface Glycoconjugates

Glycan binding often mediates extracellular macromolecular recognition events. Accurate characterization of these binding interactions can be difficult because of dissociation and scrambling that occur during purification and analysis steps. Use of photocrosslinking methods has been pursued to covalently capture glycan-dependent interactions in situ however use of metabolic glycan engineering methods to incorporate photocrosslinking sugar analogs is limited to certain cell types. Here we report an exo-enzymatic labeling method to add a diazirine-modified sialic acid (SiaDAz) to cell surface glycoconjugates. The method involves chemoenzymatic synthesis of diazirine-modified CMP-sialic acid (CMP-SiaDAz), followed by sialyltransferase-catalyzed addition of SiaDAz to desialylated cell surfaces. Cell surface SiaDAz-ylation is compatible with multiple cell types and is facilitated by endogenous extracellular sialyltransferase activity present in Daudi B cells. This method for extracellular addition of α2-6-linked SiaDAz enables UV-induced crosslinking of CD22, demonstrating the utility for covalent capture of glycan-mediated binding interactions.

Chemical modulation of cell fates: in situ regeneration

2018 ◽  
Vol 61 (10) ◽  
pp. 1137-1150 ◽  
Author(s):  
Hua Qin ◽  
Andong Zhao ◽  
Xiaobing Fu
Keyword(s):  
Cell Fates ◽  
In Situ Regeneration ◽  
Chemical Modulation

In Situ Regeneration of Au Nanocatalysts by Atmospheric-Pressure Air Plasma: Regeneration Characteristics of Square-Wave Pulsed Plasma

2017 ◽  
Vol 60 (12-14) ◽  
pp. 914-924 ◽  
Author(s):  
Bin Zhu ◽  
Jing-Lin Liu ◽  
Xiao-Song Li ◽  
Jin-Bao Liu ◽  
Xiaobing Zhu ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Pulsed Plasma ◽  
Air Plasma ◽  
Square Wave ◽  
In Situ Regeneration
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