Thermophoretic particle sampling on a TEM grid: A new design for sample preparation with high spatial accuracy

2020 ◽  
Vol 92 (9) ◽  
pp. 1330-1330
Author(s):  
F. Kunze ◽  
S. Kuns ◽  
T. Hülser ◽  
H. Wiggers ◽  
S. M. Schnurre
Keyword(s):  
Sample Preparation ◽  
Spatial Accuracy ◽  
Particle Sampling ◽  
Tem Grid

Variable Angle TEM Grid Holder for Advanced TEM Lamellae Preparation

2019 ◽  
Author(s):  
Tomáš Hrnčíř ◽  
Marek Šikula ◽  
Pavel Doleže ◽  
Claudio A. G. Savoia
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sample Preparation ◽  
Sample Holder ◽  
End Point ◽  
Transmission Electron ◽  
End Point Detection ◽  
Point Detection ◽  
User Friendly ◽  
Tem Grid

Abstract Multipurpose sample holder for advanced Transmission Electron Microscopy (TEM) sample preparation which reduces cost of the tool and most importantly simplifies the workflow is introduced. Following the current demand for user-friendly interface, semi-automated approach is aimed to be build up. Abilities to prepare advanced TEM lamellae in various geometries without rotary nanomanipulator and using various end-point detection signals are perceived as biggest advantages of this design.

scholarly journals A microfluidic device for TEM sample preparation

Lab on a Chip ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 4186-4193
Author(s):  
Janosch Hauser ◽  
Gustaf Kylberg ◽  
Mathieu Colomb-Delsuc ◽  
Göran Stemme ◽  
Ida-Maria Sintorn ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Microfluidic Device ◽  
User Interaction ◽  
Preparation Process ◽  
Single Use ◽  
Tem Grid

We present a capillary-driven microfluidic single-use device that prepares a TEM grid with minimal user interaction. The user only initiates the sample preparation process, waits for about one minute and then collects the TEM grid, ready for imaging.

Sample Preparation and Measurement of Losses in Electron Optical Analyses of Particulates in Air and Water Samples

1976 ◽  
Vol 34 ◽  
pp. 556-557
Author(s):  
R. J. Lee ◽  
D. R. DeBray ◽  
A. Szirmae
Keyword(s):  
Electron Microscope ◽  
Sample Preparation ◽  
Water Samples ◽  
Membrane Filter ◽  
Particulate Material ◽  
Transmission Electron ◽  
Significant Step ◽  
Carbon Coated ◽  
Replication Technique ◽  
Tem Grid

Electron optical analyses of the type and concentration of particles in air and water samples utilize some method of transferring the particulate material from the collection media to a transmission electron microscope (TEM) grid. The method used represents a significant step in sample preparation and may be the factor limiting the ultimate reliability of the data. We have found that a carbon replication technique in which the collection media (usually a membrane filter) is carbon-coated directly results in no measurable loss during the transfer. The salient features of the technique and a method for direct observation of any losses are described below.Under normal circumstances the sample received at the Electron Microscope laboratory consists of a membrane filter on which particulates have been deposited. This sample, if lightly loaded, may be prepared in the as-received condition.

Solid State Conformational Transitions In Peptides Modeling B. Mori Fibroin

1999 ◽  
Vol 5 (S2) ◽  
pp. 1216-1217
Author(s):  
D. Wilson ◽  
R. Valluzzi ◽  
T. Vuong ◽  
S-J Chien ◽  
S. P. Gido ◽  
...  
Keyword(s):  
Amino Acid ◽  
Sample Preparation ◽  
Silk Fibroin ◽  
Amino Acid Sequences ◽  
Carbon Substrate ◽  
Fibrous Proteins ◽  
Conformational Variability ◽  
Sample Structure ◽  
Multiple Conformations ◽  
Tem Grid

Fibrous proteins are molecules whose secondary structures are their dominant motifs due to their highly repetitive amino acid sequences. Most fibrous proteins have physiological roles as protective, connective or structural materials. Among the fibrous proteins, silks tend to have blocky structures, with crystallizable and amorphous blocks comprised of short, highly repetitive amino acid sequences. In addition, the high glycine content of silks allows them greater conformational variability than most proteins, thus Bombyx morisilk fibroin is typically polymorphic.The ability of silk fibroin to adopt multiple conformations and crystal structures makes it difficult to obtain corroborating data using multiple characterization techniques. Differences in sample preparation to accommodate different techniques can also affect the resulting sample structure. Reproducible sample preparation was achieved for IR and TEM experiments by rubbing the dried IR sample with a carbon substrate TEM grid to pick up tiny flakes of solidified peptide.

scholarly journals Particle Sampling by TEM Grid Filtration

2013 ◽  
Vol 47 (7) ◽  
pp. 767-775 ◽  
Author(s):  
B. R’mili ◽  
Olivier L. C. Le Bihan ◽  
C. Dutouquet ◽  
O. Aguerre-Charriol ◽  
E. Frejafon
Keyword(s):  
Particle Sampling ◽  
Tem Grid

scholarly journals Sublimable materials facilitate the TEM sample preparation of oil-soluble nanomaterials

2020 ◽  
Vol 50 (1) ◽  
Author(s):  
Yu-Hao Deng
Keyword(s):  
Electron Microscopy ◽  
Sample Preparation ◽  
Preparation Method ◽  
Organic Polymer ◽  
Polymer Support ◽  
Sample Preparation Method ◽  
High Quality ◽  
Transmission Electron ◽  
Tem Grid

Abstract Sample preparation is significantly important to the high-resolution transmission electron microscopy (HRTEM) characterization of nanomaterials. However, many general organic solvents can dissolve the necessary organic polymer support layer in TEM grid, which causes it difficult to obtain high-quality samples of oil-soluble nanomaterials. In this study, a new sample preparation method for oil-soluble nanomaterials has been developed by using the sublimable material as a transition layer. Experiments also show that there is no damage to TEM grids and high-quality HRTEM images can be obtained via this method. This approach paves the way to applicable HRTEM sample preparation of oil-soluble nanomaterials.

Pollutant Identification by Selected Area Electron Diffraction

1974 ◽  
Vol 32 ◽  
pp. 532-533
Author(s):  
R. E. Ferrell ◽  
G. G. Paulson ◽  
C. W. Walker
Keyword(s):  
Thermal Treatment ◽  
Electron Diffraction ◽  
Sample Preparation ◽  
Crystal Structures ◽  
Water Samples ◽  
Environmental Samples ◽  
Short Review ◽  
Selected Area Electron Diffraction ◽  
Multiple Component

Selected area electron diffraction (SAD) has been used successfully to determine crystal structures, identify traces of minerals in rocks, and characterize the phases formed during thermal treatment of micron-sized particles. There is an increased interest in the method because it has the potential capability of identifying micron-sized pollutants in air and water samples. This paper is a short review of the theory behind SAD and a discussion of the sample preparation employed for the analysis of multiple component environmental samples.

Preparation of Thin Cadmium Telluride Samples for Electron Microscope Studies

1974 ◽  
Vol 32 ◽  
pp. 548-549
Author(s):  
T. J. Magee ◽  
J. Peng ◽  
J. Bean
Keyword(s):  
Electron Microscope ◽  
Sulfuric Acid ◽  
Solid State ◽  
Sample Preparation ◽  
Cadmium Telluride ◽  
Potassium Dichromate ◽  
Optical Components ◽  
The Past ◽  
Solid State Devices

Cadmium telluride has become increasingly important in a number of technological applications, particularly in the area of laser-optical components and solid state devices, Microstructural characterizations of the material have in the past been somewhat limited because of the lack of suitable sample preparation and thinning techniques. Utilizing a modified jet thinning apparatus and a potassium dichromate-sulfuric acid thinning solution, a procedure has now been developed for obtaining thin contamination-free samples for TEM examination.

A Freeze Shadow Technique for the Preparation of Soft Paint Latexes for Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 314-315
Author(s):  
Earl R. Walter ◽  
Glen H. Bryant
Keyword(s):  
Sample Preparation ◽  
High Vacuum ◽  
Vacuum System ◽  
Latex Particles ◽  
New Methods ◽  
Diffusion Pump ◽  
Film Forming ◽  
Routine Basis ◽  
Distribution Studies ◽  
Preparation Techniques

With the development of soft, film forming latexes for use in paints and other coatings applications, it became desirable to develop new methods of sample preparation for latex particle size distribution studies with the electron microscope. Conventional latex sample preparation techniques were inadequate due to the pronounced tendency of these new soft latex particles to distort, flatten and fuse on the substrate when they dried. In order to avoid these complications and obtain electron micrographs of undistorted latex particles of soft resins, a freeze-dry, cold shadowing technique was developed. The method has now been used in our laboratory on a routine basis for several years.The cold shadowing is done in a specially constructed vacuum system, having a conventional mechanical fore pump and oil diffusion pump supplying vacuum. The system incorporates bellows type high vacuum valves to permit a prepump cycle and opening of the shadowing chamber without shutting down the oil diffusion pump. A baffeled sorption trap isolates the shadowing chamber from the pumps.

Sign in / Sign up

Export Citation Format

Share Document