Synthesis of Cell-Penetrating Peptides for Cargo Delivery

ChemInform ◽  
2006 ◽  
Vol 37 (10) ◽  
Author(s):  
Margus Pooga ◽  
Ulo Langel
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating

scholarly journals Cell-Penetrating Peptides: A Comparative Study on Lipid Affinity and Cargo Delivery Properties

2010 ◽  
Vol 3 (4) ◽  
pp. 1045-1062 ◽  
Author(s):  
Paolo Ruzza ◽  
Barbara Biondi ◽  
Anna Marchiani ◽  
Nicola Antolini ◽  
Andrea Calderan
Keyword(s):  
Comparative Study ◽  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating

scholarly journals The many futures for cell-penetrating peptides: how soon is now?

2007 ◽  
Vol 35 (4) ◽  
pp. 767-769 ◽  
Author(s):  
J. Howl ◽  
I.D. Nicholl ◽  
S. Jones
Keyword(s):  
Positive Impact ◽  
Biochemical Properties ◽  
Cell Penetrating Peptides ◽  
Protein Transduction Domains ◽  
Molecular Therapeutics ◽  
Cargo Delivery ◽  
Cell Penetrating ◽  
Transactivator Of Transcription ◽  
The Many ◽  
Hiv 1

Studies of CPPs (cell-penetrating peptides), sequences that are also commonly designated as protein transduction domains, now extend to a second decade of exciting and far-reaching discoveries. CPPs are proven vehicles for the intracellular delivery of macromolecules that include oligonucleotides, peptides and proteins, low-molecular-mass drugs, nanoparticles and liposomes. The biochemical properties of different classes of CPP, including various sequences derived from the HIV-1 Tat (transactivator of transcription) [e.g. Tat-(48–60), GRKKRRQRRRPPQ], and the homeodomain of the Drosophila homeoprotein Antennapaedia (residues 43–58, commonly named penetratin, RQIKIWFQNRRMKWKK), also provide novel insights into the fundamental mechanisms of translocation across biological membranes. Thus the efficacy of CPP-mediated cargo delivery continues to provide valuable tools for biomedical research and, as witnessed in 2007, candidate and emerging therapeutics. Thus it is anticipated that the further refinement of CPP technologies will provide drug-delivery vectors, cellular imaging tools, nanoparticulate devices and molecular therapeutics that will have a positive impact on the healthcare arena. The intention of this article is to provide both a succinct overview of current developments and applications of CPP technologies, and to illustrate key developments that the concerted efforts of the many researchers contributing to the Biochemical Society's Focused Meeting in Telford predict for the future. The accompanying papers in this issue of Biochemical Society Transactions provide additional details and appropriate references. Hopefully, the important and eagerly anticipated biomedical and clinical developments within the CPP field will occur sooner rather than later.

scholarly journals Cargo delivery kinetics of cell-penetrating peptides

2001 ◽  
Vol 1515 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Mattias Hällbrink ◽  
Anders Florén ◽  
Anna Elmquist ◽  
Margus Pooga ◽  
Tamas Bartfai ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating ◽  
Kinetics Of

scholarly journals When cationic cell-penetrating peptides meet hydrocarbons to enhance in-cell cargo delivery

2015 ◽  
Vol 21 (5) ◽  
pp. 356-369 ◽  
Author(s):  
Margherita Di Pisa ◽  
Gérard Chassaing ◽  
Jean-Marie Swiecicki
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating

scholarly journals Identification and characterization of novel enhanced cell penetrating peptides for anti-cancer cargo delivery

Oncotarget ◽  
2017 ◽  
Vol 9 (5) ◽  
pp. 5944-5957 ◽  
Author(s):  
Xiguang Zhang ◽  
Jean Yves Brossas ◽  
Christophe Parizot ◽  
Jean Marc Zini ◽  
Angelita Rebollo
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Anti Cancer ◽  
Cell Penetrating ◽  
Identification And Characterization

scholarly journals The Utilization of Cell-Penetrating Peptides in the Intracellular Delivery of Viral Nanoparticles

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2671 ◽  
Author(s):  
Jana Váňová ◽  
Alžběta Hejtmánková ◽  
Marie Hubálek Kalbáčová ◽  
Hana Španielová
Keyword(s):  
Genetic Material ◽  
Cell Types ◽  
Cell Penetrating Peptides ◽  
Target Cells ◽  
Current State ◽  
Cargo Delivery ◽  
Viral Particles ◽  
Cell Penetrating ◽  
Viral Nanoparticles ◽  
Immunogenic Properties

Viral particles (VPs) have evolved so as to efficiently enter target cells and to deliver their genetic material. The current state of knowledge allows us to use VPs in the field of biomedicine as nanoparticles that are safe, easy to manipulate, inherently biocompatible, biodegradable, and capable of transporting various cargoes into specific cells. Despite the fact that these virus-based nanoparticles constitute the most common vectors used in clinical practice, the need remains for further improvement in this area. The aim of this review is to discuss the potential for enhancing the efficiency and versatility of VPs via their functionalization with cell-penetrating peptides (CPPs), short peptides that are able to translocate across cellular membranes and to transport various substances with them. The review provides and describes various examples of and means of exploitation of CPPs in order to enhance the delivery of VPs into permissive cells and/or to allow them to enter a broad range of cell types. Moreover, it is possible that CPPs are capable of changing the immunogenic properties of VPs, which could lead to an improvement in their clinical application. The review also discusses strategies aimed at the modification of VPs by CPPs so as to create a useful cargo delivery tool.

scholarly journals Cargo-dependent cytotoxicity and delivery efficacy of cell-penetrating peptides: a comparative study

2007 ◽  
Vol 407 (2) ◽  
pp. 285-292 ◽  
Author(s):  
Samir El-Andaloussi ◽  
Peter Järver ◽  
Henrik J. Johansson ◽  
Ülo Langel
Keyword(s):  
Membrane Integrity ◽  
Cell Penetrating Peptides ◽  
Bioactive Molecules ◽  
Cargo Delivery ◽  
Wide Range ◽  
Cell Penetrating ◽  
Coupling Strategy ◽  
Transactivator Of Transcription

The use of CPPs (cell-penetrating peptides) as delivery vectors for bioactive molecules has been an emerging field since 1994 when the first CPP, penetratin, was discovered. Since then, several CPPs, including the widely used Tat (transactivator of transcription) peptide, have been developed and utilized to translocate a wide range of compounds across the plasma membrane of cells both in vivo and in vitro. Although the field has emerged as a possible future candidate for drug delivery, little attention has been given to the potential toxic side effects that these peptides might exhibit in cargo delivery. Also, no comprehensive study has been performed to evaluate the relative efficacy of single CPPs to convey different cargos. Therefore we selected three of the major CPPs, penetratin, Tat and transportan 10, and evaluated their ability to deliver commonly used cargos, including fluoresceinyl moiety, double-stranded DNA and proteins (i.e. avidin and streptavidin), and studied their effect on membrane integrity and cell viability. Our results demonstrate the unfeasibility to use the translocation efficacy of fluorescein moiety as a gauge for CPP efficiency, since the delivery properties are dependent on the cargo used. Furthermore, and no less importantly, the toxicity of CPPs depends heavily on peptide concentration, cargo molecule and coupling strategy.

scholarly journals Improving Membrane Activity and Cargo Delivery Efficacy of a Cell-Penetrating Peptide by Loading with Carboranes

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2075
Author(s):  
Tamara Lützenburg ◽  
Nele Burdina ◽  
Matthias S. Scholz ◽  
Ines Neundorf
Keyword(s):  
Secondary Structure ◽  
Cell Penetrating Peptides ◽  
Nucleic Acid Delivery ◽  
Stable Secondary Structure ◽  
Membrane Activity ◽  
Cargo Delivery ◽  
Peptide Interactions ◽  
Cell Penetrating ◽  
A Cell

Cell-penetrating peptides (CPPs) have emerged as versatile tools to increase the intracellular accumulation of different kinds of cargoes. For an efficient cellular uptake and drug delivery, their organization into a distinct and stable secondary structure at the outer surface of the plasma membrane is a hallmark and supports optimal lipid–peptide interactions. Incorporation of hydrophobic moieties, such as carboranes (CBs), has the potential to increase the lipophilicity of peptides, and thus, to facilitate the formation of secondary structures. Herein, we present synthesis and biophysical as well as biological characterization of carborane-CPP conjugates having incorporated one or more CB clusters. Our results highlight the possibility to modulate the secondary structure of CPPs by the addition of CB’s leading to constructs with altered membrane activity and promising use in terms of nucleic acid delivery.

Sign in / Sign up

Export Citation Format

Share Document