scholarly journals Identification and characterization of novel enhanced cell penetrating peptides for anti-cancer cargo delivery

Oncotarget ◽  
2017 ◽  
Vol 9 (5) ◽  
pp. 5944-5957 ◽  
Author(s):  
Xiguang Zhang ◽  
Jean Yves Brossas ◽  
Christophe Parizot ◽  
Jean Marc Zini ◽  
Angelita Rebollo
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Anti Cancer ◽  
Cell Penetrating ◽  
Identification And Characterization

scholarly journals Improving Membrane Activity and Cargo Delivery Efficacy of a Cell-Penetrating Peptide by Loading with Carboranes

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2075
Author(s):  
Tamara Lützenburg ◽  
Nele Burdina ◽  
Matthias S. Scholz ◽  
Ines Neundorf
Keyword(s):  
Secondary Structure ◽  
Cell Penetrating Peptides ◽  
Nucleic Acid Delivery ◽  
Stable Secondary Structure ◽  
Membrane Activity ◽  
Cargo Delivery ◽  
Peptide Interactions ◽  
Cell Penetrating ◽  
A Cell

Cell-penetrating peptides (CPPs) have emerged as versatile tools to increase the intracellular accumulation of different kinds of cargoes. For an efficient cellular uptake and drug delivery, their organization into a distinct and stable secondary structure at the outer surface of the plasma membrane is a hallmark and supports optimal lipid–peptide interactions. Incorporation of hydrophobic moieties, such as carboranes (CBs), has the potential to increase the lipophilicity of peptides, and thus, to facilitate the formation of secondary structures. Herein, we present synthesis and biophysical as well as biological characterization of carborane-CPP conjugates having incorporated one or more CB clusters. Our results highlight the possibility to modulate the secondary structure of CPPs by the addition of CB’s leading to constructs with altered membrane activity and promising use in terms of nucleic acid delivery.

Identification and characterization of novel protein-derived arginine-rich cell-penetrating peptides

2015 ◽  
Vol 89 ◽  
pp. 93-106 ◽  
Author(s):  
Ankur Gautam ◽  
Minakshi Sharma ◽  
Pooja Vir ◽  
Kumardeep Chaudhary ◽  
Pallavi Kapoor ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Cell Penetrating ◽  
Identification And Characterization ◽  
Novel Protein

scholarly journals Identification and Characterization of a New Family of Cell-penetrating Peptides

2011 ◽  
Vol 286 (42) ◽  
pp. 36932-36943 ◽  
Author(s):  
Laura Cascales ◽  
Sónia T. Henriques ◽  
Markus C. Kerr ◽  
Yen-Hua Huang ◽  
Matthew J. Sweet ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
New Family ◽  
Cell Penetrating ◽  
Identification And Characterization

scholarly journals β-Lactamase Tools for Establishing Cell Internalization and Cytosolic Delivery of Cell Penetrating Peptides

Biomolecules ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 51 ◽  
Author(s):  
Shane Stone ◽  
Tatjana Heinrich ◽  
Suzy Juraja ◽  
Jiulia Satiaputra ◽  
Clinton Hall ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Cell Penetrating Peptides ◽  
Stable Cell Line ◽  
Intracellular Space ◽  
Biologically Relevant ◽  
Cell Internalization ◽  
Cytosolic Delivery ◽  
Cell Penetrating ◽  
Split Protein

The ability of cell penetrating peptides (CPPs) to deliver biologically relevant cargos into cells is becoming more important as targets in the intracellular space continue to be explored. We have developed two assays based on CPP-dependent, intracellular delivery of TEM-1 β-lactamase enzyme, a functional biological molecule comparable in size to many protein therapeutics. The first assay focuses on the delivery of full-length β-lactamase to evaluate the internalization potential of a CPP sequence. The second assay uses a split-protein system where one component of β-lactamase is constitutively expressed in the cytoplasm of a stable cell line and the other component is delivered by a CPP. The delivery of a split β-lactamase component evaluates the cytosolic delivery capacity of a CPP. We demonstrate that these assays are rapid, flexible and have potential for use with any cell type and CPP sequence. Both assays are validated using canonical and novel CPPs, with limits of detection from <500 nM to 1 µM. Together, the β-lactamase assays provide compatible tools for functional characterization of CPP activity and the delivery of biological cargos into cells.

scholarly journals Cell-Penetrating Peptides: A Comparative Study on Lipid Affinity and Cargo Delivery Properties

2010 ◽  
Vol 3 (4) ◽  
pp. 1045-1062 ◽  
Author(s):  
Paolo Ruzza ◽  
Barbara Biondi ◽  
Anna Marchiani ◽  
Nicola Antolini ◽  
Andrea Calderan
Keyword(s):  
Comparative Study ◽  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating

scholarly journals The many futures for cell-penetrating peptides: how soon is now?

2007 ◽  
Vol 35 (4) ◽  
pp. 767-769 ◽  
Author(s):  
J. Howl ◽  
I.D. Nicholl ◽  
S. Jones
Keyword(s):  
Positive Impact ◽  
Biochemical Properties ◽  
Cell Penetrating Peptides ◽  
Protein Transduction Domains ◽  
Molecular Therapeutics ◽  
Cargo Delivery ◽  
Cell Penetrating ◽  
Transactivator Of Transcription ◽  
The Many ◽  
Hiv 1

Studies of CPPs (cell-penetrating peptides), sequences that are also commonly designated as protein transduction domains, now extend to a second decade of exciting and far-reaching discoveries. CPPs are proven vehicles for the intracellular delivery of macromolecules that include oligonucleotides, peptides and proteins, low-molecular-mass drugs, nanoparticles and liposomes. The biochemical properties of different classes of CPP, including various sequences derived from the HIV-1 Tat (transactivator of transcription) [e.g. Tat-(48–60), GRKKRRQRRRPPQ], and the homeodomain of the Drosophila homeoprotein Antennapaedia (residues 43–58, commonly named penetratin, RQIKIWFQNRRMKWKK), also provide novel insights into the fundamental mechanisms of translocation across biological membranes. Thus the efficacy of CPP-mediated cargo delivery continues to provide valuable tools for biomedical research and, as witnessed in 2007, candidate and emerging therapeutics. Thus it is anticipated that the further refinement of CPP technologies will provide drug-delivery vectors, cellular imaging tools, nanoparticulate devices and molecular therapeutics that will have a positive impact on the healthcare arena. The intention of this article is to provide both a succinct overview of current developments and applications of CPP technologies, and to illustrate key developments that the concerted efforts of the many researchers contributing to the Biochemical Society's Focused Meeting in Telford predict for the future. The accompanying papers in this issue of Biochemical Society Transactions provide additional details and appropriate references. Hopefully, the important and eagerly anticipated biomedical and clinical developments within the CPP field will occur sooner rather than later.

scholarly journals Cargo delivery kinetics of cell-penetrating peptides

2001 ◽  
Vol 1515 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Mattias Hällbrink ◽  
Anders Florén ◽  
Anna Elmquist ◽  
Margus Pooga ◽  
Tamas Bartfai ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating ◽  
Kinetics Of

scholarly journals Characterization of a possible uptake mechanism of selective antibacterial peptides.

1970 ◽  
Vol 60 (4) ◽  
Author(s):  
Carlos Polanco ◽  
José Lino Samaniego ◽  
Jorge Alberto Castañón-González ◽  
Thomas Buhse ◽  
Marili Leopold Sordo
Keyword(s):  
Cell Penetrating Peptides ◽  
Antibacterial Peptides ◽  
Amino Acid Residues ◽  
Uptake Mechanism ◽  
Beneficial Effects ◽  
Polarity Index ◽  
Cell Penetrating ◽  
The Subject ◽  
Living Organisms

Selective antibacterial peptides containing less than 30 amino acid residues, cationic, with amphipathic properties, have been the subject of several studies due to their active participation and beneficial effects in strengthening the immune system of all living organisms. This manuscript reports the results of a comparison between the group of selective antibacterial peptides and another group called "cell penetrating peptides". An important number of the selective antibacterial peptides are cell penetrating peptides, suggesting that their toxicity is related to their uptake mechanism. The verification of this observation also includes the adaptation of a method previously published, called Polarity index, which reproduces and confirms the action of this new set of peptides. The efficiency of this method was verified based on four different databases, yielding a high score. The verification was based exclusively on the peptides already reported in the databases which have been experimentally verified.

scholarly journals When cationic cell-penetrating peptides meet hydrocarbons to enhance in-cell cargo delivery

2015 ◽  
Vol 21 (5) ◽  
pp. 356-369 ◽  
Author(s):  
Margherita Di Pisa ◽  
Gérard Chassaing ◽  
Jean-Marie Swiecicki
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating
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