Nucleic Acid Component Analogues: Synthesis of 2′-Deoxynucleosides from 5-Substituted-4-hydroxy-6(1H)-pyrimidinones.

ChemInform ◽  
2003 ◽  
Vol 34 (30) ◽  
Author(s):  
Ahmed F. Khattab ◽  
Ahmed E.-S. Abdel Megied ◽  
Erik B. Pedersen
Keyword(s):  
Nucleic Acid ◽  
Acid Component ◽  
Nucleic Acid Component

Regulation of c-myc mRNA stability in vitro by a labile destabilizer with an essential nucleic acid component

1989 ◽  
Vol 9 (5) ◽  
pp. 1996-2006
Author(s):  
G Brewer ◽  
J Ross
Keyword(s):  
Nucleic Acid ◽  
Micrococcal Nuclease ◽  
Turnover Rates ◽  
Acid Component ◽  
Gamma Globin ◽  
Erythroleukemia Cells ◽  
Order Of Magnitude ◽  
The Stability ◽  
Nucleic Acid Component

The turnover rates of some mRNAs vary by an order of magnitude or more when cells change their growth pattern or differentiate. To identify regulatory factors that might be responsible for this variability, we investigated how cytosolic fractions affect mRNA decay in an in vitro system. A 130,000 X g supernatant (S130) from the cytosol of exponentially growing erythroleukemia cells contains a destabilizer that accelerates the decay of polysome-bound c-myc mRNA by eightfold or more compared with reactions lacking S130. The destabilizer is deficient in or absent from the S130 of cycloheximide-treated cells, indicating that it is labile or is repressed when translation is blocked. It is not a generic RNase, because it does not affect the turnover of delta-globin, gamma-globin, or histone mRNA and does not destabilize a major portion of polysomal polyadenylated mRNA. The destabilizer accelerates the turnover of the c-myc mRNA 3' region, as well as subsequent 3'-to-5' degradation of the mRNA body. It is inactivated in vitro by mild heating and by micrococcal nuclease, suggesting that it contains a nucleic acid component. c-myb mRNA is also destabilized in S130-supplemented in vitro reactions. These results imply that the stability of some mRNAs is regulated by cytosolic factors that are not associated with polysomes.

Investigations of Some Nucleic Acid Component Analogs

1999 ◽  
Vol 18 (4-5) ◽  
pp. 1119-1122 ◽  
Author(s):  
Yuri Rubin ◽  
Jerzy Leszczynski
Keyword(s):  
Nucleic Acid ◽  
Acid Component ◽  
Nucleic Acid Component

scholarly journals Regulation of c-myc mRNA stability in vitro by a labile destabilizer with an essential nucleic acid component.

1989 ◽  
Vol 9 (5) ◽  
pp. 1996-2006 ◽  
Author(s):  
G Brewer ◽  
J Ross
Keyword(s):  
Nucleic Acid ◽  
Micrococcal Nuclease ◽  
Turnover Rates ◽  
Acid Component ◽  
Gamma Globin ◽  
Erythroleukemia Cells ◽  
Order Of Magnitude ◽  
The Stability ◽  
Nucleic Acid Component

The turnover rates of some mRNAs vary by an order of magnitude or more when cells change their growth pattern or differentiate. To identify regulatory factors that might be responsible for this variability, we investigated how cytosolic fractions affect mRNA decay in an in vitro system. A 130,000 X g supernatant (S130) from the cytosol of exponentially growing erythroleukemia cells contains a destabilizer that accelerates the decay of polysome-bound c-myc mRNA by eightfold or more compared with reactions lacking S130. The destabilizer is deficient in or absent from the S130 of cycloheximide-treated cells, indicating that it is labile or is repressed when translation is blocked. It is not a generic RNase, because it does not affect the turnover of delta-globin, gamma-globin, or histone mRNA and does not destabilize a major portion of polysomal polyadenylated mRNA. The destabilizer accelerates the turnover of the c-myc mRNA 3' region, as well as subsequent 3'-to-5' degradation of the mRNA body. It is inactivated in vitro by mild heating and by micrococcal nuclease, suggesting that it contains a nucleic acid component. c-myb mRNA is also destabilized in S130-supplemented in vitro reactions. These results imply that the stability of some mRNAs is regulated by cytosolic factors that are not associated with polysomes.

THE INHIBITION OF THE PROLIFERATION OF COWPOX VIRUS IN EMBRYONATED HENS' EGGS BY CORTISONE

1963 ◽  
Vol 43 (3) ◽  
pp. 447-457 ◽  
Author(s):  
Stian Erichsen
Keyword(s):  
Nucleic Acid ◽  
Virus Protein ◽  
Cowpox Virus ◽  
Acid Component ◽  
Nucleic Acid Component ◽  
Morphological Investigations

ABSTRACT Titration of infective particles in the chorioallantoic membranes from embryonated hens' eggs showed that cortisone treatment inhibits the growth of the cowpox virus. This effect was demonstrable not earlier than 48 hours after inoculation, when the dose was 2.5 mg and not earlier than 72 hours when the dose was 0.1 mg. There was no demonstrable effect after 0.01 mg. The results confirm previous morphological investigations and were interpreted as the results of a general inhibition of the synthesis of virus protein independent of the type of the nucleic acid component of the viruses.

scholarly journals Catalytic activity of the nucleic acid component of the 1,4-α-glucan branching enzyme from rabbit muscles

1987 ◽  
Vol 15 (4) ◽  
pp. 1745-1752 ◽  
Author(s):  
T.A. Shvedova ◽  
G.A. Korneeva ◽  
V.A. Otroshchenko ◽  
T.V. Venkstern
Keyword(s):  
Catalytic Activity ◽  
Nucleic Acid ◽  
Branching Enzyme ◽  
Acid Component ◽  
Nucleic Acid Component
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